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. 1978 Sep;38(3):456–460. doi: 10.1038/bjc.1978.229

Surface antigens of rat liver epithelial cells grown in medium containing foetal bovine serum.

M J Embleton, P T Iype
PMCID: PMC2009748  PMID: 81676

Abstract

Cultured rat liver cells induced a strong antibody response in syngeneic rats, directed against foetal calf serum components which were incorporated into the liver cell surface from the cell-culture medium. This antibody could be removed by absorption with liver cells or glutaraldehyde-fixed foetal calf serum. It is possible that the antigenic cross-reactivity observed in earlier studies with cultured cells treated with carcinogens could be due to this foetal calf serum component.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baldwin R. W., Barker C. R., Embleton M. J., Glaves D., Moore M., Pimm M. V. Demonstration of cell-surface antigens on chemically induced tumors. Ann N Y Acad Sci. 1971 Jun 21;177:268–278. doi: 10.1111/j.1749-6632.1971.tb35056.x. [DOI] [PubMed] [Google Scholar]
  2. Basombrío M. A., Prehn R. T. Antigenic diversity of tumors chemically induced within the progeny of a single cell. Int J Cancer. 1972 Jul 15;10(1):1–8. doi: 10.1002/ijc.2910100102. [DOI] [PubMed] [Google Scholar]
  3. Borenfreund E., Higgins P. J., Steinglass M., Bendich A. Properties and malignant transformation of established rat liver parenchymal cells in culture. J Natl Cancer Inst. 1975 Aug;55(2):375–384. [PubMed] [Google Scholar]
  4. Colburn N. H., Bruegge W. F., Bates J. R., Gray R. H., Rossen J. D., Kelsey W. H., Shimada T. Correlation of anchorage-independent growth with tumorigenicity of chemically transformed mouse epidermal cells. Cancer Res. 1978 Mar;38(3):624–634. [PubMed] [Google Scholar]
  5. Embleton M. J., Heidelberger C. Antigenicity of clones of mouse prostate cells transformed in vitro. Int J Cancer. 1972 Jan 15;9(1):8–18. doi: 10.1002/ijc.2910090103. [DOI] [PubMed] [Google Scholar]
  6. Embleton M. J., Heidelberger C. Neoantigens on chemically transformed cloned C3H mouse embryo cells. Cancer Res. 1975 Aug;35(8):2049–2055. [PubMed] [Google Scholar]
  7. Irie R. F., Irie K., Morton D. L. Natural antibody in human serum to a neoantigen in human cultured cells grown in fetal bovine serum. J Natl Cancer Inst. 1974 Apr;52(4):1051–1058. doi: 10.1093/jnci/52.4.1051. [DOI] [PubMed] [Google Scholar]
  8. Iype P. T., Baldwin R. W., Glaves D. Cell surface antigenic changes induced in normal adult rat liver cells by carcinogen treatment in vitro. Br J Cancer. 1973 Feb;27(2):128–133. doi: 10.1038/bjc.1973.17. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Iype P. T. Cultures from adult rat liver cells. I. Establishment of monolayer cell-cultures from normal liver. J Cell Physiol. 1971 Oct;78(2):281–288. doi: 10.1002/jcp.1040780217. [DOI] [PubMed] [Google Scholar]
  10. Mondal S., Iype P. T., Griesbach L. M., Heidelberger C. Antigenicity of cells derived from mouse prostate cells after malignant transformation in vitro by carcinogenic hydrocarbons. Cancer Res. 1970 Jun;30(6):1593–1597. [PubMed] [Google Scholar]
  11. Montesano R., Saint Vincent L., Tomatis L. Malignant transformation in vitro of rat liver cells by dimethylnitrosamine and N-methyl-N'-nitro-N-nitrosoguanidine. Br J Cancer. 1973 Sep;28(3):215–220. doi: 10.1038/bjc.1973.139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Phillips E. R., Perdue J. F. Immunologic identification of fetal calf serum-derived proteins on the surfaces of cultured transformed and untransformed rat cells. Int J Cancer. 1977 Nov 15;20(5):798–804. doi: 10.1002/ijc.2910200520. [DOI] [PubMed] [Google Scholar]
  13. Williams G. M., Elliott J. M., Weisburger J. H. Carcinoma after malignant conversion in vitro of epithelial-like cells from rat liver following exposure to chemical carcinogens. Cancer Res. 1973 Mar;33(3):606–612. [PubMed] [Google Scholar]
  14. Yokota T., Sizaret P., Martel N. Tumor-specific antigens on rat liver cells transformed in vitro by chemical carcinogens. J Natl Cancer Inst. 1978 Jan;60(1):125–129. doi: 10.1093/jnci/60.1.125. [DOI] [PubMed] [Google Scholar]

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