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. 1978 Nov;38(5):573–582. doi: 10.1038/bjc.1978.252

Repopulation of gamma-irradiated Lewis lung carcinoma by malignant cells and host macrophage progenitors.

T C Stephens, G A Currie, J H Peacock
PMCID: PMC2009782  PMID: 728347

Abstract

Cellular repopulation in Lewis carcinoma irradiated with 60Co gamma-rays was examined by performing sequential cell-survival estimations using an in vitro soft-agar-colony assay. Following local irradiation (15--35 Gy) two distinct types of colony were seen: compact colonies with tightly packed cells and diffuse colonies with widely dispersed cells. Maximal diffuse colony formation in vitro was only obtained in the simultaneous presence of adequate numbers of compact colonies. After whole-body irradiation only compact colonies were observed. Only-cell survival data from compact colony counts correlated with cell survival estimated by the lung colony assay and we conclude that compact colonies are produced by clonogenic tumour cells. Cytochemical and immunological evidence showed that diffuse colonies were composed of macrophages. After local irradiation the initial kill of clonogenic tumour cells was dose dependent. At each dose level, repopulation began immediately and proceeded with a doubling time of about 1 day. Macrophage colony-forming cells (macrophage progenitors) per tumour were initially reduced by about 3 decades, but recovered very rapidly to reach pretreatment levels within 2 days. We conclude that at least two populations of clonogenic cells are present in Lewis lung carcinoma, tumour cells that repopulate irradiated tumours by in situ proliferation and host-macrophage progenitors that repopulate locally irradiated tumours by infiltration. The hazards of confusing host and tumour cell colonies in in vitro assay systems are stressed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Courtenay V. D. A soft agar colony assay for Lewis lung tumour and B16 melanoma taken directly from the mouse. Br J Cancer. 1976 Jul;34(1):39–45. doi: 10.1038/bjc.1976.119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Eccles S. A., Alexander P. Macrophage content of tumours in relation to metastatic spread and host immune reaction. Nature. 1974 Aug 23;250(5468):667–669. doi: 10.1038/250667a0. [DOI] [PubMed] [Google Scholar]
  3. Evans R., Alexander P. Cooperation of immune lymphoid cells with macrophages in tumour immunity. Nature. 1970 Nov 14;228(5272):620–622. doi: 10.1038/228620a0. [DOI] [PubMed] [Google Scholar]
  4. Evans R. Macrophages in syngeneic animal tumours. Transplantation. 1972 Oct;14(4):468–473. doi: 10.1097/00007890-197210000-00011. [DOI] [PubMed] [Google Scholar]
  5. Haskill J. S., Proctor J. W., Yamamura Y. Host responses with solid tumors. I. Monocytic effector cells within rat sarcomas. J Natl Cancer Inst. 1975 Feb;54(2):387–393. [PubMed] [Google Scholar]
  6. Lin H. S., Stewart C. C. Peritoneal exudate cells. I. Growth requirement of cells capable of forming colonies in soft agar. J Cell Physiol. 1974 Jun;83(3):369–378. doi: 10.1002/jcp.1040830307. [DOI] [PubMed] [Google Scholar]
  7. Osserman E. F., Lawlor D. P. Serum and urinary lysozyme (muramidase) in monocytic and monomyelocytic leukemia. J Exp Med. 1966 Nov 1;124(5):921–952. doi: 10.1084/jem.124.5.921. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Shipley W. U., Stanley J. A., Courtenay V. D., Field S. B. Repair of radiation damage in Lewis lung carcinoma cells following in situ treatment with fast neutrons and gamma-rays. Cancer Res. 1975 Apr;35(4):932–938. [PubMed] [Google Scholar]
  9. Steel G. G., Adams K. Stem-cell survival and tumor control in the Lewis lung carcinoma. Cancer Res. 1975 Jun;35(6):1530–1535. [PubMed] [Google Scholar]
  10. Steel G. G., Hill R. P., Peckham M. J. Combined radiotherapy--chemotherapy of Lewis lung carcinoma. Int J Radiat Oncol Biol Phys. 1978 Jan-Feb;4(1-2):49–52. doi: 10.1016/0360-3016(78)90114-1. [DOI] [PubMed] [Google Scholar]
  11. Stephens T. C., Peacock J. H. Tumour volume response, initial cell kill and cellular repopulation in B16 melanoma treated with cyclophosphamide and 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea. Br J Cancer. 1977 Sep;36(3):313–321. doi: 10.1038/bjc.1977.195. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Stewart C. C., Beetham K. L. Cytocidal activity and proliferative ability of macrophages infiltrating the EMT6 tumor. Int J Cancer. 1978 Aug 15;22(2):152–159. doi: 10.1002/ijc.2910220208. [DOI] [PubMed] [Google Scholar]
  13. Thomson J. E., Rauth A. M. An in vitro assay to measure the viability of KHT tumor cells not previously exposed to culture conditions. Radiat Res. 1974 May;58(2):262–276. [PubMed] [Google Scholar]
  14. Yam L. T., Li C. Y., Crosby W. H. Cytochemical identification of monocytes and granulocytes. Am J Clin Pathol. 1971 Mar;55(3):283–290. doi: 10.1093/ajcp/55.3.283. [DOI] [PubMed] [Google Scholar]

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