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. 1979 Dec;40(6):848–855. doi: 10.1038/bjc.1979.275

Carcinoembryonic antigen in endoscopic brush specimens from benign and malignant gastric lesions.

J Lindgren, P Sipponen, K Seppälä, S Tarpila, S Nordling, T Wahlström, M Seppälä
PMCID: PMC2010141  PMID: 393292

Abstract

The measurement of carcinoembryonic antigen (CEA) in serum and endoscopic brush specimens was evaluated for the differential diagnosis of malignant and nonmalignant gastric disease. Brush specimens were studied from 33 patients with gastric cancer and 36 patients with benign gastric lesions or apparently normal gastric mucosa. Demonstrable CEA immunoreactivity was found by radioimmunoassay in brush specimens from 24/33 cancer patients (73%) and from 23/36 patients with benign lesions (64%). Patients with CEA+ tissue in the immunoperoxidase test had somewhat higher CEA concentrations in the brush specimens than cases with CEA- biopsy tissue, although overlap was considerable. Thirty-five per cent of cancer patients had both a positive tissue CEA reaction and a CEA/DNA ratio greater than 10 ng/micrograms, whilst patients with benign lesions had only 15% of positives by these criteria (0.01 greater than P greater than 0.001). The serum CEA concentration was above the upper normal level of 5 ng/ml in 2/39 patients, both of whom had gastric cancer. The CEA immunoreactive material from benign and malignant lesions eluted in gel filtration on Sephadex G-200 in the same volume as CEA purified from liver metastases of cancer of the colon, showing that a glycoprotein sharing immunological and physicochemical properties with CEA is present both in malignant and nonmalignant lesions of the gastric mucosa, and that there is considerable overlapping in the amount of CEA. The estimation of CEA in gastric-brush specimens is therefore of limited value in the differential diagnosis of benign and malignant gastric lesions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bunn P. A., Jr, Cohen M. I., Widerlite L., Nugent J. L., Matthews M. J., Minna J. D. Simultaneous gastric and plasma immunoreactive plasma carcinoembryonic antigen in 108 patients undergoing gastroscopy. Gastroenterology. 1979 Apr;76(4):734–741. [PubMed] [Google Scholar]
  2. Burns J. Background staining and sensitivity of the unlabelled antibody-enzyme (PAP) method. Comparison with the peroxidase labelled antibody sandwich method using formalin fixed paraffin embedded material. Histochemistry. 1975 Jun 5;43(3):291–294. doi: 10.1007/BF00499711. [DOI] [PubMed] [Google Scholar]
  3. Burtin P., Chavanel G., Hirsch-Marie H. Characterization of a second normal antigen that cross-reacts with CEA. J Immunol. 1973 Dec;111(6):1926–1928. [PubMed] [Google Scholar]
  4. Burtin P., Sabine M. C., Chavanel G. A comparative study of the localization of CEA and NCA2 in cancerous and normal gastrointestinal tissues. Int J Cancer. 1977 May 15;19(5):634–641. doi: 10.1002/ijc.2910190507. [DOI] [PubMed] [Google Scholar]
  5. Darcy D. A., Turberville C., James R. Immunological study of carcinoembryonic antigen (CEA) and a related glycoprotein. Br J Cancer. 1973 Aug;28(2):147–160. doi: 10.1038/bjc.1973.132. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Denk H., Tappeiner G., Davidovits A., Eckerstorfer R., Holzner J. H. Carcinoembryonic antigen and blood group substances in carcinomas of the stomach and colon. J Natl Cancer Inst. 1974 Oct;53(4):933–942. doi: 10.1093/jnci/53.4.933. [DOI] [PubMed] [Google Scholar]
  7. DiMagno E. P., Malagelada J. R., Moertel C. G., Go V. L. Prospective evaluation of the pancreatic secretion of immunoreactive carcinoembryonic antigen, enzyme, and bicarbonate in patients suspected of having pancreatic cancer. Gastroenterology. 1977 Sep;73(3):457–461. [PubMed] [Google Scholar]
  8. GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Go V. L., Ammon H. V., Holtermuller K. H., Krag E., Phillips S. F. Quantification of carcinoembryonic antigen-like activities in normal, human gastrointestinal secretions. Cancer. 1975 Dec;36(6 Suppl):2346–2350. doi: 10.1002/1097-0142(197512)36:6<2346::aid-cncr2820360611>3.0.co;2-w. [DOI] [PubMed] [Google Scholar]
  10. Hammarström S., Engvall E., Johansson B. G., Svensson S., Sundblad G., Goldstein I. J. Nature of the tumor-associated determinant(s) of carcinoembryonic antigen. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1528–1532. doi: 10.1073/pnas.72.4.1528. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Isaacson P., Judd M. A. Carcinoembryonic antigen (CEA) in the normal human small intestine: a light and electron microscopic study. Gut. 1977 Oct;18(10):786–791. doi: 10.1136/gut.18.10.786. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kim Y. D., Hirata A. A. Carcinoembryonic antigen-like substances in human cavity fluids. Immunol Commun. 1976;5(7-8):619–629. doi: 10.3109/08820137609033870. [DOI] [PubMed] [Google Scholar]
  13. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  14. Mach J. P., Pusztaszeri G. Carcinoembryonic antigen (CEA): demonstration of a partial identity between CEA and a normal glycoprotein. Immunochemistry. 1972 Oct;9(10):1031–1034. doi: 10.1016/0019-2791(72)90113-9. [DOI] [PubMed] [Google Scholar]
  15. Minton J. P., Martin E. W., Jr The use of serial CEA determinations to predict recurrence of colon cancer and when to do a second-look operation. Cancer. 1978 Sep;42(3 Suppl):1422–1427. doi: 10.1002/1097-0142(197809)42:3+<1422::aid-cncr2820420807>3.0.co;2-1. [DOI] [PubMed] [Google Scholar]
  16. Molnar I. G., Vandevoorde J. P., Gitnick G. L. CEA levels in fluids bathing gastrointestinal tumors. Gastroenterology. 1976 Apr;70(4):513–515. [PubMed] [Google Scholar]
  17. Neville A. M., Cooper E. H. Biochemical monitoring of cancer. A review. Ann Clin Biochem. 1976 Jan;13(1):283–305. doi: 10.1177/000456327601300101. [DOI] [PubMed] [Google Scholar]
  18. Newman E. S., Petras S. E., Georgiadis A., Hansen H. J. Proceedings: Interrelationship of carcinoembryonic antigen and colon carcinoma antigen-3. Cancer Res. 1974 Aug;34(8):2125–2130. [PubMed] [Google Scholar]
  19. Orjasaeter H. Demonstration of carcinoembryonic antigens (CEA), nonspecific cross-reacting antigens (NCA) and an associated alpha protein in normal human tissues and fluids by immunodiffusion techniques. Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Jun;82(3):387–395. [PubMed] [Google Scholar]
  20. Primus F. J., Wang R. H., Sharkey R. M., Goldenberg D. M. Detection of carcinoembryonic antigen in tissue sections by immunoperoxidase. J Immunol Methods. 1975 Sep;8(3):267–275. doi: 10.1016/0022-1759(75)90121-0. [DOI] [PubMed] [Google Scholar]
  21. Rutanen E. M., Lindgren J., Sipponen P., Stenman U. P., Saksela E., Seppäla M. Carcinoembryonic antigen in malignant and nonmalignant gynecologic tumors: circulating levels and tissue localization. Cancer. 1978 Aug;42(2):581–590. doi: 10.1002/1097-0142(197808)42:2<581::aid-cncr2820420226>3.0.co;2-g. [DOI] [PubMed] [Google Scholar]
  22. Svenberg T. Carcinoembryonic antigen-like substances of human bile. Isolation and partial characterization. Int J Cancer. 1976 May 15;17(5):588–596. doi: 10.1002/ijc.2910170506. [DOI] [PubMed] [Google Scholar]
  23. Turberville C., Darcy D. A., Laurence D. J., Johns E. W., Neville A. M. Studies on carcinoembryonic antigen (CEA) and a related glycoprotein, CCEA-2. Preparation and chemical characterisation. Immunochemistry. 1973 Dec;10(12):841–843. doi: 10.1016/0019-2791(73)90189-4. [DOI] [PubMed] [Google Scholar]
  24. Vuento M., Engvall E., Seppälä M., Ruoslahti E. Isolation from human gastric juice of an antigen closely related to the carcinoembryonic antigen. Int J Cancer. 1976 Aug 15;18(2):156–160. doi: 10.1002/ijc.2910180204. [DOI] [PubMed] [Google Scholar]
  25. Vuento M., Ruoslahti E., Pihko H., Svenberg T., Ihamäki T., Siurala M. Carcinoembryonic antigen-like substance in gastric juice. Immunochemistry. 1976 Apr;13(4):313–316. doi: 10.1016/0019-2791(76)90340-2. [DOI] [PubMed] [Google Scholar]
  26. von Kleist S., Chavanel G., Burtin P. Identification of an antigen from normal human tissue that crossreacts with the carcinoembryonic antigen. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2492–2494. doi: 10.1073/pnas.69.9.2492. [DOI] [PMC free article] [PubMed] [Google Scholar]

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