Abstract
The SCM test was established as originally described, and an attempt was made to evaluate it using myelin basic proteins. Various later modifications described by the original authors were incorporated as they were communicated to us. In separate studies attempts were also made to overcome some of the problems which seemed inherent in the technique. In the small series for which valid results were obtained we were unable to confirm the original claim that the method discriminates between cancer patient lymphocytes and those from non-cancer subjects with almost 99% reliability. Indeed, although differences were found between the mean SCM values of cancer patients and of healthy controls, these differences were not significant.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Balding P., Light P. A., Preece A. W. Response of human lymphocytes to PHA and tumour-associated antigens as detected by fluorescence polarization. Br J Cancer. 1980 Jan;41(1):73–85. doi: 10.1038/bjc.1980.9. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Biozzi G., Stiffel C., Mouton D., Bouthillier Y., Decreusefond C. A kinetic study of antibody producing cells in the spleen of mice immunized intravenously with sheep erythrocytes. Immunology. 1968 Jan;14(1):7–20. [PMC free article] [PubMed] [Google Scholar]
- Cercek L., Cercek B. Application of the phenomenon of changes in the structuredness of cytoplasmic matrix (SCM) in the diagnosis of malignant disorders: a review. Eur J Cancer. 1977 Sep;13(9):903–915. doi: 10.1016/0014-2964(77)90166-9. [DOI] [PubMed] [Google Scholar]
- Cercek L., Cercek B., Franklin C. I. Biophysical differentiation between lymphocytes from healthy donors, patients with malignant diseases and other disorders. Br J Cancer. 1974 May;29(5):345–352. doi: 10.1038/bjc.1974.81. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cercek L., Cercek B., Ockey C. H. Fluorescein excitation and emission polarization spectra in living cells: changes during the cell cycle. Biophys J. 1978 Sep;23(3):395–405. doi: 10.1016/S0006-3495(78)85458-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Deibler G. E., Martenson R. E., Kies M. W. Large scale preparation of myelin basic protein from central nervous tissue of several mammalian species. Prep Biochem. 1972;2(2):139–165. doi: 10.1080/00327487208061467. [DOI] [PubMed] [Google Scholar]
- Hashimoto Y., Takaku F., Yamanaka T. Changes in structuredness of cytoplasmic matrix in single stimulated lymphocytes from healthy donors and patients with non-malignant and malignant diseases. Br J Cancer. 1979 Jul;40(1):156–160. doi: 10.1038/bjc.1979.152. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Udkoff R., Norman A. Polarization of fluorescein fluorescence in single cells. J Histochem Cytochem. 1979 Jan;27(1):49–55. doi: 10.1177/27.1.438505. [DOI] [PubMed] [Google Scholar]