Skip to main content
PMC home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1980 May;41(5):695–704. doi: 10.1038/bjc.1980.131

Effect of host-cell interactions on clonogenic carcinoma cells in human malignant effusions.

R N Buick, S E Fry, S E Salmon
PMCID: PMC2010329  PMID: 7426298

Abstract

We have studied the clonogenic capacity of tumour cells in agar from 38 malignant effusions from 31 patients with epithelial tumours. Colony formation of unfractionated cells, varies considerably from patient to patient, and is positively correlated with the percentage of tumour cells in the sample. Clonogenicity was shown to be reduced in 8/9 cases by removal of plastic-adherent and iron-phagocytic cells. In the ninth case, increased clonogenicity occurred after this procedure. When the autologous adherent cells were removed from the effusion and used in reconstitution experiments as an underlayer in a two-layer agar system, they were able to reverse the effect of the initial fractionation in a dose-dependent fashion. This indicates cellular communication based on release of a diffusible product of plastic-adherent cells. Morphological, phagocytic and prostaglandin-synthetic analysis of the fractions involved in the reconstitution experiments implicate the macrophage as the operative cell in this interaction. However, an accessory role for lymphoid cells or tumour cells themselves cannot be excluded.

Full text

PDF
695

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Buick R. N., Fry S. E., Salmon S. E. Application of in vitro soft agar techniques for growth of tumor cells to the study of colon cancer. Cancer. 1980 Mar 15;45(5 Suppl):1238–1242. doi: 10.1002/1097-0142(19800315)45:5+<1238::aid-cncr2820451333>3.0.co;2-r. [DOI] [PubMed] [Google Scholar]
  2. Buick R. N., Stanisic T. H., Fry S. E., Salmon S. E., Trent J. M., Krasovich P. Development of an agar-methyl cellulose clonogenic assay for cells in transitional cell carcinoma of the human bladder. Cancer Res. 1979 Dec;39(12):5051–5056. [PubMed] [Google Scholar]
  3. Fidler I. J., Brodey R. S., Bech-Nielsen S. In vitro immune stimulation-inhibition to spontaneous canine tumors of various histologic types. J Immunol. 1974 Mar;112(3):1051–1060. [PubMed] [Google Scholar]
  4. Hamburger A. W., Salmon S. E., Kim M. B., Trent J. M., Soehnlen B. J., Alberts D. S., Schmidt H. J. Direct cloning of human ovarian carcinoma cells in agar. Cancer Res. 1978 Oct;38(10):3438–3444. [PubMed] [Google Scholar]
  5. Hamburger A. W., Salmon S. E. Primary bioassay of human tumor stem cells. Science. 1977 Jul 29;197(4302):461–463. doi: 10.1126/science.560061. [DOI] [PubMed] [Google Scholar]
  6. Holden H. T., Haskill J. S., Kirchner H., Herberman R. B. Two functionally distinct anti-tumor effector cells isolated from primary murine sarcoma virus-induced tumors. J Immunol. 1976 Aug;117(2):440–446. [PubMed] [Google Scholar]
  7. Kurland J. I., Pelus L. M., Ralph P., Bockman R. S., Moore M. A. Induction of prostaglandin E synthesis in normal and neoplastic macrophages: role for colony-stimulating factor(s) distinct from effects on myeloid progenitor cell proliferation. Proc Natl Acad Sci U S A. 1979 May;76(5):2326–2330. doi: 10.1073/pnas.76.5.2326. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Light R. W., Erozan Y. S., Ball W. C., Jr Cells in pleural fluid. Their value in differential diagnosis. Arch Intern Med. 1973 Dec;132(6):854–860. [PubMed] [Google Scholar]
  9. Mantovani A., Peri G., Polentarutti N., Bolis G., Mangioni C., Spreafico F. Effects on in vitro tumor growth of macrophages isolated from human ascitic ovarian tumors. Int J Cancer. 1979 Feb;23(2):157–164. doi: 10.1002/ijc.2910230204. [DOI] [PubMed] [Google Scholar]
  10. Norbury K. C. In vitro stimulation and inhibition of tumor cell growth mediated by different lymphoid cell populations. Cancer Res. 1977 May;37(5):1408–1415. [PubMed] [Google Scholar]
  11. Okabe T., Sato N., Kondo Y., Asano S., Ohsawa N., Kosaka K., Ueyama Y. Establishment and characterization of a human cancer cell line that produces human colony-stimulating factor. Cancer Res. 1978 Nov;38(11 Pt 1):3910–3917. [PubMed] [Google Scholar]
  12. Prehn R. T. Immunostimulation of the lymphodependent phase of neoplastic growth. J Natl Cancer Inst. 1977 Oct;59(4):1043–1049. doi: 10.1093/jnci/59.4.1043. [DOI] [PubMed] [Google Scholar]
  13. Reddy J. K., Rao M. S. Enhancement by Wy-14,643, a hepatic peroxisome proliferator, of diethylnitrosamine-initiated hepatic tumorigenesis in the rat. Br J Cancer. 1978 Oct;38(4):537–543. doi: 10.1038/bjc.1978.241. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Salmon S. E., Buick R. N. Preparation of permanent slides of intact soft-agar colony cultures of hematopoietic and tumor stem cells. Cancer Res. 1979 Mar;39(3):1133–1136. [PubMed] [Google Scholar]
  15. Salmon S. E., Hamburger A. W. Immunoproliferation and cancer: a common macrophage-derived promoter substance. Lancet. 1978 Jun 17;1(8077):1289–1290. doi: 10.1016/s0140-6736(78)91270-9. [DOI] [PubMed] [Google Scholar]
  16. Underwood J. C. Lymphoreticular infiltration in human tumours: prognostic and biological implications: a review. Br J Cancer. 1974 Dec;30(6):538–548. doi: 10.1038/bjc.1974.233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wood G. W., Gillespie G. Y. Studies on the role of macrophages in regulation of growth and metastasis of murine chemically induced fibrosarcomas. Int J Cancer. 1975 Dec 15;16(6):1022–1029. doi: 10.1002/ijc.2910160616. [DOI] [PubMed] [Google Scholar]
  18. Yam L. T., Li C. Y., Crosby W. H. Cytochemical identification of monocytes and granulocytes. Am J Clin Pathol. 1971 Mar;55(3):283–290. doi: 10.1093/ajcp/55.3.283. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES