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. 1980 Aug;42(2):275–283. doi: 10.1038/bjc.1980.227

Significance of cytotoxic lymphocytes after various immunizing procedures in a virus-induced non-producer syngeneic system: correlation between in vitro and in vivo lytic activity.

Y Pioch, M Gerber, B Serrou
PMCID: PMC2010375  PMID: 6448617

Abstract

An originally virus-induced, non-producer tumour system has been studied in relation to humoral and cellular cytotoxic responses to transplantation and other immunization techniques. In all experimental groups cytotoxic lymphocytes (CTL) were observed either directly or after mixed culture of lymphocytes and tumour cells (MLTC). Except for C'-dependent cytotoxic antibodies in mice immunized by irradiated cells, no antibody-mediated cytotoxicity was observed. In 2 protocols (transplantation and immunization by mitomycin-treated cells) CTL in vitro were not protective. In a third protocol (immunization by irradiated cells) CTL afforded partial protection and other factors appeared to be involved. The best in vivo protection was induced by immunization consequent on early surgical removal of a small number of transplanted tumour cells. This study provides lines of evidence for the effectiveness of protection supplied by CTL in well-defined conditions. Comparison with other modes of immunization indicated that these conditions were related to the quantity and to the characteristics of antigen involved.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benjamini E., Fong S., Erickson C., Leung C. Y., Rennick D., Scibienski R. J. Immunity to lymphoid tumors in syngeneic mice by immunization with mitomycin C-treated cells. J Immunol. 1977 Feb;118(2):685–693. [PubMed] [Google Scholar]
  2. Bosslet K., Schirrmacher V., Shantz G. Tumor metastases and cell-mediated immunity in a model system in DBA/2 mice. VI. Similar specificity patterns of protective anti-tumor immunity in vivo and of cytolytic T cells in vitro. Int J Cancer. 1979 Sep 15;24(3):303–313. doi: 10.1002/ijc.2910240306. [DOI] [PubMed] [Google Scholar]
  3. Carlson G. A., Terres G. Antibody-induced killing in vivo of L1210/MTX-R cells quantitated in passively immunized mice with 131I-iododeoxyuridine-labeled cells and whole-body measurement of retained radioactivity. J Immunol. 1976 Sep;117(3):822–829. [PubMed] [Google Scholar]
  4. Cerottini J. C., Engers H. D., Macdonald H. R., Brunner T. Generation of cytotoxic T lymphocytes in vitro. I. Response of normal and immune mouse spleen cells in mixed leukocyte cultures. J Exp Med. 1974 Sep 1;140(3):703–717. doi: 10.1084/jem.140.3.703. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Creighton W. D., Lambert P. H., Miescher P. A. Detection of antibodies and soluble antigen-antibody complexes by precipitation with polyethylene glycol. J Immunol. 1973 Oct;111(4):1219–1227. [PubMed] [Google Scholar]
  6. Dunkley M., Miller R. G., Shortman K. A modified 51Cr release assay for cytotoxic lymphocytes. J Immunol Methods. 1974 Dec;6(1-2):39–51. doi: 10.1016/0022-1759(74)90088-x. [DOI] [PubMed] [Google Scholar]
  7. Dunlop M. B., Doherty P. C., Zinkernagel R. M., Blanden R. V. Cytotoxic T cell response to lymphocytic choriomeningitis virus. Properties of precursors of effector T cells, primary effector T cells and memory T cells in vitro and in vivo. Immunology. 1977 Sep;33(3):361–368. [PMC free article] [PubMed] [Google Scholar]
  8. Fitch F. W., Engers H. D., MacDonald H. R., Cerottini J. C., Brunner K. T. Generation of cytotoxic T lymphocytes in vitro. VI. Effect of cell density on response in mixed leukocyte cultures. J Immunol. 1975 Dec;115(6):1688–1694. [PubMed] [Google Scholar]
  9. Gerber M. J., Brown E. R. In vivo and in vitro evaluation of immune response of hamsters to immunization and transplants of autochthonous reticulum cell sarcoma. Cancer Res. 1973 Nov;33(11):3029–3035. [PubMed] [Google Scholar]
  10. Ghaffar A., Calder E. A., Irvine W. J. K cell cytotoxicity against antibody-coated chicken erythrocytes in tumor-bearing mice: its development with progressively growing tumor and the effect of immunization against the tumor. J Immunol. 1976 Feb;116(2):315–318. [PubMed] [Google Scholar]
  11. Glynn J. P., McCoy J. L., Fefer A. Cross-resistance to the transplantation of syngeneic Friend, Moloney, and Rauscher virus-induced tumors. Cancer Res. 1968 Mar;28(3):434–439. [PubMed] [Google Scholar]
  12. Hunninghake G. W., Fauci A. S. Quantitative and qualitative effects of cyclophosphamide administration on circulating polymorphonuclear leucocytes. Immunology. 1976 Jul;31(1):139–144. [PMC free article] [PubMed] [Google Scholar]
  13. Leclerc J. C., Gomard E., Plata F., Levy J. P. Cell-mediated immune reaction against tumors induced by oncornaviruses. II. Nature of the effector cells in tumor-cell cytolysis. Int J Cancer. 1973 Mar 15;11(2):426–432. doi: 10.1002/ijc.2910110220. [DOI] [PubMed] [Google Scholar]
  14. Levy J. P., Leclerc J. C. The murine sarcoma virus-induced tumor: exception or general model in tumor immunology? Adv Cancer Res. 1977;24:1–66. doi: 10.1016/s0065-230x(08)61012-x. [DOI] [PubMed] [Google Scholar]
  15. MacDonald H. R., Cerottini J. C., Brunner K. T. Generation of cytotoxic T lymphocytes in vitro. III. Velocity sedimentation studies of the differentiation and fate of effector cells in long-term mixed leukocyte cultures. J Exp Med. 1974 Dec 1;140(6):1511–1521. doi: 10.1084/jem.140.6.1511. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Macdonald H. R., Engers H. D., Cerottini J. C., Brunner K. T. Generation of cytotoxic T lymphocytes in vitro. II. Effect of repeated exposure to alloantigens on the cytotoxic activity of long-term mixed leukocyte cultures. J Exp Med. 1974 Sep 1;140(3):718–730. doi: 10.1084/jem.140.3.718. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Robertson H. T., Black P. H. Changes in surface antigens of SV40-virus transformed cells. Proc Soc Exp Biol Med. 1969 Feb;130(2):363–370. doi: 10.3181/00379727-130-33556. [DOI] [PubMed] [Google Scholar]
  18. Ting C. C., Bonnard G. D. Cell-mediated immunity to friend virus-induced leukemia. IV. In vitro generation of primary and secondary cell-mediated cytotoxic responses. J Immunol. 1976 May;116(5):1419–1425. [PubMed] [Google Scholar]
  19. Ting C. C., Rodgigues D., Bushar G. S., Herberman R. B. Cell-mediated immunity to Friend virus-induced leukemia. II. Characteristics of primary cell-mediated cytotoxic response. J Immunol. 1976 Jan;116(1):236–243. [PubMed] [Google Scholar]
  20. WINN H. J. Immune mechanisms in homotransplantation. II. Quantitative assay of the immunologic activity of lymphoid cells stimulated by tumor homografts. J Immunol. 1961 Feb;86:228–239. [PubMed] [Google Scholar]
  21. Wernet D., Lilly F. Genetic regulation of the antibody response to H-2Db alloantigens in mice. I. Differences in activation of helper T cells in C57BL/10 and BALB/c congenic strains. J Exp Med. 1975 Mar 1;141(3):573–583. doi: 10.1084/jem.141.3.573. [DOI] [PMC free article] [PubMed] [Google Scholar]

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