Skip to main content
NCBI home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1980 Oct;42(4):596–609. doi: 10.1038/bjc.1980.284

Surface proteins in normal and transformed rat liver epithelial cells in culture.

G A Bannikov, L Saint Vincent, R Montesano
PMCID: PMC2010454  PMID: 7053205

Abstract

The pattern of surface proteins of different types of normal and transformed rat liver cells have been studied in culture by means of lactoperoxidase-catalysed iodination procedures, followed by SDS-gel electrophoresis. The cells examined were primary cultures of epithelial liver cells, long-term cultures of epithelial liver cells, in vitro transformed epithelial liver cell lines and liver tumour-cell lines; mesenchymal cells from liver and skin were also examined. The principal surface proteins of primary cultures of epithelial cells from adult or neonatal rats had components with mol. wts of 140,000-160,000, 100,000 and 40,000-70,000. A band that had the same position as fibronectin from mesenchymal cells was also present and this band, as well as other iodinated components, were less sensitive to trypsin than fibroblastic fibronectin. A similar pattern of iodinated proteins was seen in long-term cultures of epithelial liver cells, with a great reduction in the number and intensity of the bands in the mol. wt region below 100,000. Almost all the in vitro transformed and tumour epithelial cell lines contain a protein with a mol. wt 135,000 as one of the major iodinated bands, and in contrast to the observation in transformed fibroblasts, the fibronectin was retained by most of these transformed cell lines.

Full text

PDF
596

Images in this article

599Fig. 1
on p.599
601Fig. 2
on p.601
602Fig. 3
on p.602

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benenson A., Kapeller M., Doljanski F. Surface proteins of fibroblast and sarcoma cells--their shedding and trypsin susceptibility. Isr J Med Sci. 1977 Aug;13(8):852–858. [PubMed] [Google Scholar]
  2. Chen L. B., Maitland N., Gallimore P. H., McDougall J. K. Detection of the large external transformation-sensitive protein on some epithelial cells. Exp Cell Res. 1977 Apr;106(1):39–46. doi: 10.1016/0014-4827(77)90238-5. [DOI] [PubMed] [Google Scholar]
  3. Crouch E., Balian G., Holbrook K., Duksin D., Bornstein P. Amniotic fluid fibronectin. Characterization and synthesis by cells in culture. J Cell Biol. 1978 Sep;78(3):701–715. doi: 10.1083/jcb.78.3.701. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hubbard A. L., Cohn Z. A. Externally disposed plasma membrane proteins. I. Enzymatic iodination of mouse L cells. J Cell Biol. 1975 Feb;64(2):438–460. doi: 10.1083/jcb.64.2.438. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hynes R. O. Cell surface proteins and malignant transformation. Biochim Biophys Acta. 1976 Apr 30;458(1):73–107. doi: 10.1016/0304-419x(76)90015-9. [DOI] [PubMed] [Google Scholar]
  6. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  7. Laishes B. A., Williams G. M. Conditions affecting primary cell cultures of functional adult rat hepatocytes. 1. The effect of insulin. In Vitro. 1976 Jul;12(7):521–532. doi: 10.1007/BF02796495. [DOI] [PubMed] [Google Scholar]
  8. Laishes B. A., Williams G. M. Conditions affecting primary cell cultures of functional adult rat hepatocytes. II. Dexamethasone enhanced longevity and maintenance of morphology. In Vitro. 1976 Dec;12(12):821–832. doi: 10.1007/BF02796367. [DOI] [PubMed] [Google Scholar]
  9. Linder E., Vaheri A., Ruoslahti E., Wartiovaara J. Distribution of fibroblast surface antigen in the developing chick embryo. J Exp Med. 1975 Jul 1;142(1):41–49. doi: 10.1084/jem.142.1.41. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Marceau N., Robert A., Mailhot D. The major surface protein of epithelial cells from newborn and adult rat livers in primary cultures. Biochem Biophys Res Commun. 1977 Apr 25;75(4):1092–1097. doi: 10.1016/0006-291x(77)91494-2. [DOI] [PubMed] [Google Scholar]
  11. Montesano R., Drevon C., Kuroki T., Saint Vincent L., Handleman S., Sanford K. K., DeFeo D., Weinstein I. B. Test for malignant transformation of rat liver cells in culture: cytology, growth in soft agar, and production of plasminogen activator. J Natl Cancer Inst. 1977 Dec;59(6):1651–1658. doi: 10.1093/jnci/59.6.1651. [DOI] [PubMed] [Google Scholar]
  12. Montesano R., Saint Vincent L., Drevon C., Tomatis L. Production of epithelial and mesenchymal tumours with rat liver cells transformed in vitro. Int J Cancer. 1975 Oct 15;16(4):550–558. doi: 10.1002/ijc.2910160405. [DOI] [PubMed] [Google Scholar]
  13. Montesano R., Saint Vincent L., Tomatis L. Malignant transformation in vitro of rat liver cells by dimethylnitrosamine and N-methyl-N'-nitro-N-nitrosoguanidine. Br J Cancer. 1973 Sep;28(3):215–220. doi: 10.1038/bjc.1973.139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Quaroni A., Isselbacher K. J., Ruoslahti E. Fibronectin synthesis by epithelial crypt cells of rat small intestine. Proc Natl Acad Sci U S A. 1978 Nov;75(11):5548–5552. doi: 10.1073/pnas.75.11.5548. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sirica A. E., Richards W., Tsukada Y., Sattler C. A., Pitot H. C. Fetal phenotypic expression by adult rat hepatocytes on collagen gel/nylon meshes. Proc Natl Acad Sci U S A. 1979 Jan;76(1):283–287. doi: 10.1073/pnas.76.1.283. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Stenman S., Vaheri A. Distribution of a major connective tissue protein, fibronectin, in normal human tissues. J Exp Med. 1978 Apr 1;147(4):1054–1064. doi: 10.1084/jem.147.4.1054. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Vaheri A., Mosher D. F. High molecular weight, cell surface-associated glycoprotein (fibronectin) lost in malignant transformation. Biochim Biophys Acta. 1978 Sep 18;516(1):1–25. doi: 10.1016/0304-419x(78)90002-1. [DOI] [PubMed] [Google Scholar]
  18. Vesterinen E., Leinikki P., Saksela E. Cytopathogenicity of cytomegalovirus to human ecto- and endocervical epithelial cells in vitro. Acta Cytol. 1975 Sep-Oct;19(5):473–481. [PubMed] [Google Scholar]
  19. Wigley C. B., Summerhayes I. C. Loss of LETS protein is not a marker for salivary gland or bladder epithelial cell transformation. Exp Cell Res. 1979 Feb;118(2):394–398. doi: 10.1016/0014-4827(79)90164-2. [DOI] [PubMed] [Google Scholar]
  20. Williams G. M., Gunn J. M. Long-term cell culture of adult rat liver epithelial cells. Exp Cell Res. 1974 Nov;89(1):139–142. doi: 10.1016/0014-4827(74)90196-7. [DOI] [PubMed] [Google Scholar]
  21. Yamada K. M. Immunological characterization of a major transformation-sensitive fibroblast cell surface glycoprotein. Localization, redistribution, and role in cell shape. J Cell Biol. 1978 Aug;78(2):520–541. doi: 10.1083/jcb.78.2.520. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Yamada K. M., Weston J. A. Isolation of a major cell surface glycoprotein from fibroblasts. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3492–3496. doi: 10.1073/pnas.71.9.3492. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Zardi L., Siri A., Carnemolla B., Cosulich E., Viale G., Santi L. A simplified procedure for the preparation of antibodies to serum fibronectin. J Immunol Methods. 1980;34(2):155–165. doi: 10.1016/0022-1759(80)90169-6. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES