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. 1981 Feb;43(2):149–156. doi: 10.1038/bjc.1981.23

Human myeloid leukaemic cell interactions in vitro with normal myeloid colonies.

G Spitzer, D S Verma, M Beran, A R Zander, K A Dicke, K B McCredie, S Siegel, S Tindle
PMCID: PMC2010528  PMID: 6970587

Abstract

To determine whether myeloid leukaemic cells could inhibit normal myeloid colony formation, leukaemic cells at concentrations ranging from 0.5 to 8 X 10(6)/ml were co-cultured in agar but separated by a 1 ml underlayer from 10(5) low-density (less than 1.077 g/ml) nonadherent normal marrow cells. Inhibition of normal-marrow myeloid colony formation occurred regularly at high cell concentrations (8 X 10(6)) at a leukaemic:normal cell ratio of 80:1. This suppression persisted with addition of indomethacin (10(-6)M). On the other hand, both low leukaemic cell numbers and irradiated leukaemic cells frequently stimulated normal colony growth. No inhibitor of colony growth could be detected in leukaemic-conditioned media, and absorption of colony-stimulating activity (CSA) with leukaemic cells improved CSA activity. These experiments point to the difficulty in unravelling the effect of leukaemic cells on normal haemopoiesis (both inhibitory and stimulatory) by in vitro agar culture.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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