Skip to main content
NCBI home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1980 Dec;42(6):915–921. doi: 10.1038/bjc.1980.340

Surface properties of cells isolated from non-metastasizing and metastasizing hamster lymphosarcomas.

D Guy, A L Latner, G V Sherbet, G A Turner
PMCID: PMC2010587  PMID: 6893935

Abstract

Present evidence suggests that the cell surface has an important role in metastasis. To examine this idea further, the surface properties of single cells isolated from the primary growths of a liver-metastasizing (ML) and a non-metastasizing (NML) lymphosarcoma were compared for adhesion to cell monolayers, cytopherometry, isoelectric focusing, adhesion to immobilized lectins and surface labelling with lactoperoxidase-catalysed radioiodination. It was found that the ML cells had increased adhesion to 3 out of 4 of the monolayers studies; a lower overall surface charge but greater peripheral concentrations of charge; and increased surface expression of the fucose moiety. No consistent difference between the two cell types was detected in the electrophoretic pattern of the labelled surface proteins. These findings are discussed in the light of present knowledge of the cell surface, and it is concluded that the significance of any of the observed changes in relation to metastasis has yet to be established.

Full text

PDF
915

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bosmann H. B., Bieber G. F., Brown A. E., Case K. R., Gersten D. M., Kimmerer T. W., Lione A. Biochemical parameters correlated with tumour cell implantation. Nature. 1973 Dec 21;246(5434):487–489. doi: 10.1038/246487a0. [DOI] [PubMed] [Google Scholar]
  2. Brunson K. W., Beattie G., Nicolsin G. L. Selection and altered properties of brain-colonising metastatic melanoma. Nature. 1978 Apr 6;272(5653):543–545. doi: 10.1038/272543a0. [DOI] [PubMed] [Google Scholar]
  3. Buck C. A., Glick M. C., Warren L. A comparative study of glycoproteins from the surface of control and Rous sarcoma virus transformed hamster cells. Biochemistry. 1970 Nov 10;9(23):4567–4576. doi: 10.1021/bi00825a016. [DOI] [PubMed] [Google Scholar]
  4. Carter R. L., Gershon R. K. Studies on homotransplantable lymphomas in hamsters. I. Histologic responses in lymphoid tissues and their relationship to metastasis. Am J Pathol. 1966 Oct;49(4):637–655. [PMC free article] [PubMed] [Google Scholar]
  5. Chatterjee S. K., Kim U. Fucosyltransferase activity in metastasizing and nonmetastasizing rat mammary carcinomas. J Natl Cancer Inst. 1978 Jul;61(1):151–162. doi: 10.1093/jnci/61.1.151. [DOI] [PubMed] [Google Scholar]
  6. Fidler I. J. Tumor heterogeneity and the biology of cancer invasion and metastasis. Cancer Res. 1978 Sep;38(9):2651–2660. [PubMed] [Google Scholar]
  7. Gershon R. K., Carter R. L., Kondo K. On concomitant immunity in tumour-bearing hamsters. Nature. 1967 Feb 18;213(5077):674–676. doi: 10.1038/213674a0. [DOI] [PubMed] [Google Scholar]
  8. Greaves M. F., Tursi A., Playfair J. H., Torrigiani G., Zamir R., Roitt I. M. Immunosuppressive potency and in-vitro activity of antilymphocyte globulin. Lancet. 1969 Jan 11;1(7585):68–72. doi: 10.1016/s0140-6736(69)91089-7. [DOI] [PubMed] [Google Scholar]
  9. Guy D., Latner A. L., Turner G. A. A simple lectin-mediated cell-adhesion method for investigating the cell surface. Exp Cell Biol. 1979;47(4):312–319. doi: 10.1159/000162950. [DOI] [PubMed] [Google Scholar]
  10. Guy D., Latner A. L., Turner G. A. Radioiodination studies of tumour cell-surface proteins after different disaggregation procedures. Br J Cancer. 1977 Aug;36(2):166–172. doi: 10.1038/bjc.1977.173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Guy D., Latner A. L., Turner G. A. Surface protein distributions in cells isolated from solid tumours and their metastases. Br J Cancer. 1979 Oct;40(4):634–640. doi: 10.1038/bjc.1979.229. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Latner A. L., Turner G. A. Surface modification and electrophoresis of normal and transformed BHK21 cells. J Cell Sci. 1974 Jan;14(1):203–214. doi: 10.1242/jcs.14.1.203. [DOI] [PubMed] [Google Scholar]
  13. Nicolson G. L. Trans-membrane control of the receptors on normal and tumor cells. II. Surface changes associated with transformation and malignancy. Biochim Biophys Acta. 1976 Apr 30;458(1):1–72. doi: 10.1016/0304-419x(76)90014-7. [DOI] [PubMed] [Google Scholar]
  14. Poste G., Fidler I. J. The pathogenesis of cancer metastasis. Nature. 1980 Jan 10;283(5743):139–146. doi: 10.1038/283139a0. [DOI] [PubMed] [Google Scholar]
  15. Raz A., Bucana C., McLellan W., Fidler I. J. Distribution of membrane anionic sites on B16 melanoma variants with differing lung colonising potential. Nature. 1980 Mar 27;284(5754):363–364. doi: 10.1038/284363a0. [DOI] [PubMed] [Google Scholar]
  16. Raz A., McLellan W. L., Hart I. R., Bucana C. D., Hoyer L. C., Sela B. A., Dragsten P., Fidler I. J. Cell surface properties of B16 melanoma variants with differing metastatic potential. Cancer Res. 1980 May;40(5):1645–1651. [PubMed] [Google Scholar]
  17. Reading C. L., Belloni P. N., Nicolson G. L. Selection and in vivo properties of lectin-attachment variants of malignant murine lymphosarcoma cell lines. J Natl Cancer Inst. 1980 May;64(5):1241–1249. [PubMed] [Google Scholar]
  18. Sherbet G. V., Lakshmi M. S. Characterisation of Escherichia coli cell surface by isoelectric equilibrium analysis. Biochim Biophys Acta. 1973 Feb 27;298(1):50–58. doi: 10.1016/0005-2736(73)90008-4. [DOI] [PubMed] [Google Scholar]
  19. Tao T., Matter A., Vogel K., Burger M. M. Liver-colonizing melanoma cells selected from B-16 melanoma. Int J Cancer. 1979 Jun 15;23(6):854–857. doi: 10.1002/ijc.2910230618. [DOI] [PubMed] [Google Scholar]
  20. Walther B. T., Ohman R., Roseman S. A quantitative assay for intercellular adhesion. Proc Natl Acad Sci U S A. 1973 May;70(5):1569–1573. doi: 10.1073/pnas.70.5.1569. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Warren L., Zeidman I., Buck C. A. The surface glycoproteins of a mouse melanoma growing in culture and as a solid tumor in vivo. Cancer Res. 1975 Aug;35(8):2186–2190. [PubMed] [Google Scholar]
  22. Winkelhake J. L., Nicolson G. L. Determination of adhesive properties of variant metastatic melanoma cells to BALB/3T3 cells and their virus-transformed derivatives by a monolayer attachment assay. J Natl Cancer Inst. 1976 Feb;56(2):285–291. doi: 10.1093/jnci/56.2.285. [DOI] [PubMed] [Google Scholar]
  23. van Beek W. P., Smets L. A., Emmelot P. Increased sialic acid density in surface glycoprotein of transformed and malignant cells--a general phenomenon? Cancer Res. 1973 Nov;33(11):2913–2922. [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES