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. 1981 May;43(5):575–581. doi: 10.1038/bjc.1981.86

Localization of human breast-carcinoma xenografts using antibodies to carcinoembryonic antigen.

V Moshakis, M J Bailey, M G Ormerod, J H Westwood, A M Neville
PMCID: PMC2010670  PMID: 7248144

Abstract

Affinity-purified antibodies to carcinoembryonic antigen (CEA) have been injected into immune-suppressed mice bearing xenografts of human breast tumours. It has been shown that the antibodies localized in the tumours but not in normal tissues. The degree of tumour localization correlates with the amount of tumour CEA, and is unaffected by levels of circulating CEA or CEA/anti-CEA immune complexes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bailey M. J., Gazet J. C., Peckham M. J. Human breast-cancer xenografts in immune-suppressed mice. Br J Cancer. 1980 Oct;42(4):524–529. doi: 10.1038/bjc.1980.275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bailey M. J., Ormerod M. G., Imrie S. F., Humphreys J., Roberts J. D., Gazet J. C., Neville A. M. Comparative functional histopathology of human breast carcinoma xenografts. Br J Cancer. 1981 Feb;43(2):125–134. doi: 10.1038/bjc.1981.20. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Coombes R. C., Powles T. J., Gazet J. C., Nash A. G., Ford H. T., McKinna A., Neville A. M. Assessment of biochemical tests to screen for metastases in patients with breast cancer. Lancet. 1980 Feb 9;1(8163):296–297. doi: 10.1016/s0140-6736(80)90790-4. [DOI] [PubMed] [Google Scholar]
  4. Dykes P. W., Hine K. R., Bradwell A. R., Blackburn J. C., Reeder T. A., Drolc Z., Booth S. N. Localisation of tumour deposits by external scanning after injection of radiolabelled anti-carcinoembryonic antigen. Br Med J. 1980 Jan 26;280(6209):220–222. doi: 10.1136/bmj.280.6209.220. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. GOLD P., FREEDMAN S. O. DEMONSTRATION OF TUMOR-SPECIFIC ANTIGENS IN HUMAN COLONIC CARCINOMATA BY IMMUNOLOGICAL TOLERANCE AND ABSORPTION TECHNIQUES. J Exp Med. 1965 Mar 1;121:439–462. doi: 10.1084/jem.121.3.439. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Goldenberg D. M., DeLand F., Kim E., Bennett S., Primus F. J., van Nagell J. R., Jr, Estes N., DeSimone P., Rayburn P. Use of radiolabeled antibodies to carcinoembryonic antigen for the detection and localization of diverse cancers by external photoscanning. N Engl J Med. 1978 Jun 22;298(25):1384–1386. doi: 10.1056/NEJM197806222982503. [DOI] [PubMed] [Google Scholar]
  8. Heyderman E., Neville A. M. A shorter immunoperoxidase technique for the demonstration of carcinoembryonic antigen and other cell products. J Clin Pathol. 1977 Feb;30(2):138–140. doi: 10.1136/jcp.30.2.138. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Heyderman E., Steele K., Ormerod M. G. A new antigen on the epithelial membrane: its immunoperoxidase localisation in normal and neoplastic tissue. J Clin Pathol. 1979 Jan;32(1):35–39. doi: 10.1136/jcp.32.1.35. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Laurence D. J., Stevens U., Bettelheim R., Darcy D., Leese C., Turberville C., Alexander P., Johns E. W., Neville A. M. Role of plasma carcinoembryonic antigen in diagnosis of gastrointestinal, mammary, and bronchial carcinoma. Br Med J. 1972 Sep 9;3(5827):605–609. doi: 10.1136/bmj.3.5827.605. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mach J. P., Carrel S., Forni M., Ritschard J., Donath A., Alberto P. Tumor localization of radiolabeled antibodies against carcinoembryonic antigen in patients with carcinoma: a critical evaluation. N Engl J Med. 1980 Jul 3;303(1):5–10. doi: 10.1056/NEJM198007033030102. [DOI] [PubMed] [Google Scholar]
  12. Mach J. P., Carrel S., Merenda C., Sordat B., Cerottini J. C. In vivo localisation of radiolabelled antibodies to carcinoembryonic antigen in human colon carcinoma grafted into nude mice. Nature. 1974 Apr 19;248(5450):704–706. doi: 10.1038/248704a0. [DOI] [PubMed] [Google Scholar]
  13. Mantovani A., Evans R., Alexander P. Non-specific cytotoxicity of spleen cells in mice bearing transplanted chemically induced fibrosarcomas. Br J Cancer. 1977 Jul;36(1):35–40. doi: 10.1038/bjc.1977.151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Moshakis V., McIlhinney R., Raghavan D., Neville A. M. Monoclonal antibodies to detect human tumours: an experimental approach. J Clin Pathol. 1981 Mar;34(3):314–319. doi: 10.1136/jcp.34.3.314. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. PRESSMAN D. Radiolabeled antibodies. Ann N Y Acad Sci. 1957 Dec 16;69(4):644–650. doi: 10.1111/j.1749-6632.1957.tb49702.x. [DOI] [PubMed] [Google Scholar]
  16. Primus F. J., Bennett S. J., Kim E. E., DeLand F. H., Zahn M. C., Goldenberg D. M. Circulating immune complexes in cancer patients receiving goat radiolocalizing antibodies to carcinoembryonic antigen. Cancer Res. 1980 Mar;40(3):497–501. [PubMed] [Google Scholar]
  17. Primus F. J., Wang R. H., Goldenberg D. M., Hansen H. J. Localization of human GW-39 tumors in hamsters by radiolabeled heterospecific antibody to carcinoembryonic antigen. Cancer Res. 1973 Nov;33(11):2977–2982. [PubMed] [Google Scholar]
  18. Steel G. G., Courtenay V. D., Rostom A. Y. Improved immune-suppression techniques for the exongrafting of human tumours. Br J Cancer. 1978 Feb;37(2):224–230. doi: 10.1038/bjc.1978.30. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Tormey D. C., Waalkes T. P. Clinical correlation between CEA and breast cancer. Cancer. 1978 Sep;42(3 Suppl):1507–1511. doi: 10.1002/1097-0142(197809)42:3+<1507::aid-cncr2820420823>3.0.co;2-w. [DOI] [PubMed] [Google Scholar]
  20. Westwood J. H., Thomas P. Studies on the structure and immunological activity of carcinoembryonic antigen - the role of disulphide bonds. Br J Cancer. 1975 Dec;32(6):708–719. doi: 10.1038/bjc.1975.282. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. van Nagell J. R., Jr, Kim E., Casper S., Primus F. J., Bennett S., DeLand F. H., Goldenberg D. M. Radioimmunodetection of primary and metastatic ovarian cancer using radiolabeled antibodies to carcinoembryonic antigen. Cancer Res. 1980 Mar;40(3):502–506. [PubMed] [Google Scholar]

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