Skip to main content
NCBI home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1981 Jun;43(6):856–863. doi: 10.1038/bjc.1981.125

In vitro susceptibilities of normal human skin fibroblasts to oncoviruses, and the decreased susceptibility to HSV of fibroblasts from untreated Hodgkin's patients.

P Ebbesen, B F Vestergaard, R Ting, S Haahr, J Genner, A Svejgaard
PMCID: PMC2010722  PMID: 6166307

Abstract

Fibroblast cultures established from the skin of 56 healthy controls and 15 untreated Stages I and II Hodgkin's patients (HD) were studied in their 3rd, 4th and 5th in vitro passage with respect to transformation with Simian sarcoma virus (SSV) and SV40 and with respect to replication of herpes simplex virus (HSV) Types 1 and 2, pox virus and interferon release. Susceptibility to the 5 viruses varied independently, except for an inverse correlation between susceptibility to SSV and HSV. HD cultures showed a depressed replication of both types of HSV. There was a borderline (P = 0.02) correlation between magnitude of HSV replication and presence of HL-A type B-w44, but this does not explain the HD control difference. Furthermore, the level of serum antibodies to HSV common antigen was not related to magnitude of in vitro replication. The results thus speak against generally enhanced cellular susceptibility to HSV as a reason for the high titres of serum antibodies to HSV in HD patients.

Full text

PDF
856

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bassin R. H., Gerwin B. I., Duran-Troise G., Gisselbrecht S., Rein A. Murine sarcoma virus pseudotypes acquire a determinant specifying N or B tropism from leukaemia virus during rescue. Nature. 1975 Jul 17;256(5514):223–225. doi: 10.1038/256223a0. [DOI] [PubMed] [Google Scholar]
  2. Brautbar C., Pellegrino M. A., Ferrone S., Reisfeld R. A., Payne R., Hayflick L. Fate of HL-A antigens in aging cultured human diploid cell strains. II. Quantitative absorption studies. Exp Cell Res. 1973 Apr;78(2):367–375. doi: 10.1016/0014-4827(73)90081-5. [DOI] [PubMed] [Google Scholar]
  3. EVANS C. A., CHAMBERS V. C., SMITH W. M., BYATT P. H. Growth of neurotropic viruses in extraneural tissues. V. Survey of tissues of various animal species for capacity to support multiplication of poliomyelitis virus in vitro. J Infect Dis. 1954 May-Jun;94(3):273–283. doi: 10.1093/infdis/94.3.273. [DOI] [PubMed] [Google Scholar]
  4. Evans A. S., Carvalho R. P., Frost P., Jamra M., Pozzi D. H. Epstein-Barr virus infections in Brazil. II. Hodgkin's disease. J Natl Cancer Inst. 1978 Jul;61(1):19–26. doi: 10.1093/jnci/61.1.19. [DOI] [PubMed] [Google Scholar]
  5. Haahr S. The occurrence of virus and interferon in the spleen, serum and brain in mice after experimental infection with West Nile virus. Acta Pathol Microbiol Scand. 1968;74(3):445–457. doi: 10.1111/j.1699-0463.1968.tb03498.x. [DOI] [PubMed] [Google Scholar]
  6. Henderson B. E., Dworsky R., Menck H., Alena B., Henle W., Henle G., Terasaki P., Newell G. R., Rawlings W., Kinnear B. K. Case-control study of Hodgkin's disease. II. Herpesvirus group antibody titers and HL-A type. J Natl Cancer Inst. 1973 Nov;51(5):1443–1447. [PubMed] [Google Scholar]
  7. Hesse J., Levine P. H., Ebbesen P., Connelly R. R., Mordhorst C. H. A case control study on immunity to two Epstein-Barr virus-associated antigens, and to herpes simplex virus and adenovirus in a population-based group of patients with Hodgkin's disease in Denmark, 1971-73. Int J Cancer. 1977 Jan;19(1):49–58. doi: 10.1002/ijc.2910190108. [DOI] [PubMed] [Google Scholar]
  8. Kaplan H. S., Goodenow R. S., Epstein A. L., Gartner S., Declève A., Rosenthal P. N. Isolation of a type C RNA virus from an established human histiocytic lymphoma cell line. Proc Natl Acad Sci U S A. 1977 Jun;74(6):2564–2568. doi: 10.1073/pnas.74.6.2564. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Levine P. H., Ablashi D. V., Berard C. W., Carbone P. P., Waggoner D. E., Malan L. Elevated antibody titers to Epstein-Barr virus in Hodgkin's disease. Cancer. 1971 Feb;27(2):416–421. doi: 10.1002/1097-0142(197102)27:2<416::aid-cncr2820270227>3.0.co;2-w. [DOI] [PubMed] [Google Scholar]
  10. Old L. J., Boyse E. A. Current enigmas in cancer research. Harvey Lect. 1973;67:273–315. [PubMed] [Google Scholar]
  11. Rager-Zisman B., Allison A. C. Mechanism of immunologic resistance to herpes simplex virus 1 (HSV-1) infection. J Immunol. 1976 Jan;116(1):35–40. [PubMed] [Google Scholar]
  12. Russell A. S., Schlaut J. HL-A transplantation antigens in subjects susceptible to recrudescent herpes labialis. Tissue Antigens. 1975 Oct;6(4):257–261. doi: 10.1111/j.1399-0039.1975.tb00640.x. [DOI] [PubMed] [Google Scholar]
  13. Simpson R. W., Obijeski J. F. Conditional lethal mutants of vesicular stomatitis virus. I. Phenotypic characterization of single and double mutants exhibiting host restriction and temperature sensitivity. Virology. 1974 Feb;57(2):357–368. doi: 10.1016/0042-6822(74)90175-5. [DOI] [PubMed] [Google Scholar]
  14. Spencer M. J., Cherry J. D., Terasaki P. I. HL-A antigens and antibody response after influenza A vaccination. Decreased response associated with HL-A type W16. N Engl J Med. 1976 Jan 1;294(1):13–16. doi: 10.1056/NEJM197601012940104. [DOI] [PubMed] [Google Scholar]
  15. Takemoto K. K., Ting R. C., Ozer H. L., Fabisch P. Establishment of a cell line from an inbred mouse strain for viral transformation studies: simian virus 40 transformation and tumor production. J Natl Cancer Inst. 1968 Dec;41(6):1401–1409. [PubMed] [Google Scholar]
  16. Theilen G. H., Gould D., Fowler M., Dungworth D. L. C-type virus in tumor tissue of a woolly monkey (Lagothrix spp.) with fibrosarcoma. J Natl Cancer Inst. 1971 Oct;47(4):881–889. [PubMed] [Google Scholar]
  17. Vestergaard B. F., Hornsleth A., Pedersen S. N. Occurrence of herpes- and adenovirus antibodies in patients with carcinoma of the cervix uteri. Measurement of antibodies to herpesvirus hominis (types 1 and 2), cytomegalovirus, EB-virus, and adenovirus. Cancer. 1972 Jul;30(1):68–74. doi: 10.1002/1097-0142(197207)30:1<68::aid-cncr2820300111>3.0.co;2-0. [DOI] [PubMed] [Google Scholar]
  18. Vogt P. K., Ishizaki R. Reciprocal patterns of genetic resistance to avian tumor viruses in two lines of chickens. Virology. 1965 Aug;26(4):664–672. doi: 10.1016/0042-6822(65)90329-6. [DOI] [PubMed] [Google Scholar]
  19. Wahl S. M., Wahl L. M., McCarthy J. B. Lymphocyte-mediated activation of fibroblast proliferation and collagen production. J Immunol. 1978 Sep;121(3):942–946. [PubMed] [Google Scholar]
  20. Wernet P. Human Ia-type alloantigens: methods of detection, aspects of chemistry and biology, markers for disease states. Transplant Rev. 1976;30:271–298. doi: 10.1111/j.1600-065x.1976.tb00223.x. [DOI] [PubMed] [Google Scholar]
  21. de Maeyer E., de Maeyer-Guignard J., Hall W. T., Bailey D. W. A locus affecting circulating interferon levels induced by mouse mammary tumour virus. J Gen Virol. 1974 May;23(2):209–211. doi: 10.1099/0022-1317-23-2-209. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES