Skip to main content
NCBI home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1981 Sep;44(3):433–439. doi: 10.1038/bjc.1981.202

Multiplication of human NHIK 3025 cells exposed to porphyrins in combination with light.

T Christensen
PMCID: PMC2010756  PMID: 7197158

Abstract

Cells from the established line NHIK 3025 were exposed to haematoporphyrin derivative and light. After this photodynamic treatment the first interphase of surviving cells was prolonged. Furthermore, a pronounced effect on the progression through the first mitosis was observed. Mainly the duration of metaphase was increased. Some of the cells were irreversibly arrested in mitosis and the cells that were able to complete mitosis after treatment multiplied in the subsequent generations at the same rate as the control. Cells treated in the late stages of the mitosis went out of mitosis at the same rate as the control. This indicates that the treatment with porphyrins and light induces a block in a specific stage of mitosis.

Full text

PDF
433

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Christensen T., Moan J., Wibe E., Oftebro R. Photodynamic effect of haematoporphyrin throughout the cell cycle of the human cell line NHIK 3025 cultivated in vitro. Br J Cancer. 1979 Jan;39(1):64–68. doi: 10.1038/bjc.1979.8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dougherty T. J., Kaufman J. E., Goldfarb A., Weishaupt K. R., Boyle D., Mittleman A. Photoradiation therapy for the treatment of malignant tumors. Cancer Res. 1978 Aug;38(8):2628–2635. [PubMed] [Google Scholar]
  3. Gomer C. J., Smith D. M. Photoinactivation of Chinese hamster cells by hematoporphyrin derivative and red light. Photochem Photobiol. 1980 Sep;32(3):341–348. doi: 10.1111/j.1751-1097.1980.tb03772.x. [DOI] [PubMed] [Google Scholar]
  4. Kelly J. F., Snell M. E. Hematoporphyrin derivative: a possible aid in the diagnosis and therapy of carcinoma of the bladder. J Urol. 1976 Feb;115(2):150–151. doi: 10.1016/s0022-5347(17)59108-9. [DOI] [PubMed] [Google Scholar]
  5. Kessel D. Effects of photoactivated porphyrins at the cell surface of leukemia L1210 cells. Biochemistry. 1977 Jul 26;16(15):3443–3449. doi: 10.1021/bi00634a023. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. LIPSON R. L., BALDES E. J., OLSEN A. M. The use of a derivative of hematoporhyrin in tumor detection. J Natl Cancer Inst. 1961 Jan;26:1–11. [PubMed] [Google Scholar]
  7. Moan J., Christensen T. Photodynamic inactivation of cancer cells in vitro. Effect of irradiation temperature and dose fractionation. Cancer Lett. 1979 May;6(6):331–335. doi: 10.1016/s0304-3835(79)80090-7. [DOI] [PubMed] [Google Scholar]
  8. Moan J., Pettersen E. O., Christensen T. The mechanism of photodynamic inactivation of human cells in vitro in the presence of haematoporphyrin. Br J Cancer. 1979 Apr;39(4):398–407. doi: 10.1038/bjc.1979.72. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Moan J., Waksvik H., Christensen T. DNA single-strand breaks and sister chromatid exchanges induced by treatment with hematoporphyrin and light or by x-rays in human NHIK 3025 cells. Cancer Res. 1980 Aug;40(8 Pt 1):2915–2918. [PubMed] [Google Scholar]
  10. PUCK T. T., CIECIURA S. J., FISHER H. W. Clonal growth in vitro of human cells with fibroblastic morphology; comparison of growth and genetic characteristics of single epithelioid and fibroblast-like cells from a variety of human organs. J Exp Med. 1957 Jul 1;106(1):145–158. doi: 10.1084/jem.106.1.145. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Pettersen E. O., Bakke O., Lindmo T., Oftebro R. Cell cycle characteristics of synchronized and asynchronous populations of human cells and effect of cooling of selected mitotic cells. Cell Tissue Kinet. 1977 Nov;10(6):511–522. doi: 10.1111/j.1365-2184.1977.tb00309.x. [DOI] [PubMed] [Google Scholar]
  12. Pettersen E. O., Oftebro R., Brustad T. X-ray inactivation of human cells in tissue culture under aerobic and extremely hypoxic conditions in the presence and absence of TMPN. Int J Radiat Biol Relat Stud Phys Chem Med. 1973 Sep;24(3):285–296. doi: 10.1080/09553007314551121. [DOI] [PubMed] [Google Scholar]
  13. Rønning O. W., Pettersen E. O., Seglen P. O. Protein synthesis and protein degradation through the cell cycle of human NHIK 3025 cells in vitro. Exp Cell Res. 1979 Oct 1;123(1):63–72. doi: 10.1016/0014-4827(79)90421-x. [DOI] [PubMed] [Google Scholar]
  14. Wakulchik S. D., Schiltz J. R., Bickers D. R. Photolysis of protoporphyrin-treated human fibroblasts in vitro: studies on the mechanism. J Lab Clin Med. 1980 Jul;96(1):158–167. [PubMed] [Google Scholar]
  15. Wibe E., Oftebro R., Christensen T., Laland S. G., Pettersen E. O., Lindmo T. Inhibitory effects of the new mitotic inhibitor 5-chloropyrimidin-2-one and of vincristine on human cells in vitro. Cancer Res. 1978 Mar;38(3):560–565. [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES