Abstract
Tumour cells from a polyoma-induced ascitic tumour were fractionated on the basis of the electrical charge on the cell surface by free-flow electrophoresis. Several characteristics of tumour cells have been investigated: (1) differences in the proliferation and antigenicity within the tumour at any point in time; (2) variation in proliferative potential with the ageing of the tumour. In early ascitic tumours, electrophoretically fractionated cells exhibit very similar proliferative characteristics. However, most DNA synthesis was found in slow-moving cells. The behaviour of older tumours was different. Proliferative potential and DNA synthesis were weaker and restricted to slow-moving cells, suggesting that fast-moving cells in older tumours were resting cells. An enrichment in immunoglobulin-bearing cells was also found in slow-moving cell fractions, supporting the hypothesis of variability in expression of tumour-specific antigens. The role of cell-surface properties and cell kinetics is discussed in relation to electrical surface charge, which might be involved in cell dissemination and metastasis. Thus, free-flow electrophoresis represents a satisfactory approach to isolate tumour cell subpopulations with characteristics such as high proliferative potential or increased expression of tumour antigens.
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Selected References
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- Bischoff P., Robert F., Donner M. Single cell analysis of changes in electrokinetic properties of a growing ascitic tumor. Cancer Biochem Biophys. 1979;4(2):79–85. [PubMed] [Google Scholar]
- Borochov H., Shinitzky M. Vertical displacement of membrane proteins mediated by changes in microviscosity. Proc Natl Acad Sci U S A. 1976 Dec;73(12):4526–4530. doi: 10.1073/pnas.73.12.4526. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Donner M., Raffoux C., Streiff F. Characterization of the human peripheral effector cells mediating antibody dependent cellular cytotoxicity against allogenic cells. Clin Exp Immunol. 1979 Dec;38(3):549–560. [PMC free article] [PubMed] [Google Scholar]
- Hannig K., Wirth H., Meyer B. H., Zeiller K. [Free-flow electrophoresis. I. Theoretical and experimental investigations of the influence of mechanical and electrokinetic variables on the efficiency of the method]. Hoppe Seylers Z Physiol Chem. 1975 Aug;356(8):1209–1223. doi: 10.1515/bchm2.1975.356.2.1209. [DOI] [PubMed] [Google Scholar]
- Hansson M., Kärre K., Kiessling R., Roder J., Andersson B., Häyry P. Natural NK-cell targets in the mouse thymus: characteristics of the sensitive cell population. J Immunol. 1979 Aug;123(2):765–771. [PubMed] [Google Scholar]
- Kerbel R. S. Implications of immunological heterogeneity of tumours. Nature. 1979 Aug 2;280(5721):358–360. doi: 10.1038/280358a0. [DOI] [PubMed] [Google Scholar]
- Mayhew E., O'Grady E. A. Electrophoretic mobilities of tissue culture cells in exponential and parasynchronous growth. Nature. 1965 Jul 3;207(992):86–87. doi: 10.1038/207086a0. [DOI] [PubMed] [Google Scholar]
- Mehrishi J. N. Molecular aspects of the mammalian cell surface. Prog Biophys Mol Biol. 1972;25:1–70. doi: 10.1016/0079-6107(72)90013-2. [DOI] [PubMed] [Google Scholar]
- Pearlstein E., Salk P. L., Yogeeswaran G., Karpatkin S. Correlation between spontaneous metastatic potential, platelet-aggregating activity of cell surface extracts, and cell surface sialylation in 10 metastatic-variant derivatives of a rat renal sarcoma cell line. Proc Natl Acad Sci U S A. 1980 Jul;77(7):4336–4339. doi: 10.1073/pnas.77.7.4336. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pimm M. V., Embleton M. J., Baldwin R. W. Multiple antigenic specificities within primary 3-methylcholanthrene-induced rat sarcomas and metastases. Int J Cancer. 1980 May 15;25(5):621–629. doi: 10.1002/ijc.2910250512. [DOI] [PubMed] [Google Scholar]
- Shinitzky M., Skornick Y., Haran-Ghera N. Effective tumor immunization induced by cells of elevated membrane-lipid microviscosity. Proc Natl Acad Sci U S A. 1979 Oct;76(10):5313–5316. doi: 10.1073/pnas.76.10.5313. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Turner G. A., Sherbet G. V., Wildridge M. A comparison of the electrokinetic properties of metastasizing and non-metastasizing forms of a hamster lymphosarcoma. Exp Cell Biol. 1980;48(5):385–392. doi: 10.1159/000163004. [DOI] [PubMed] [Google Scholar]
- Yogeeswaran G. Incorporation of asialo GM2 and gangliosides in cell surface of cultured metastatic and nonmetastatic BALB/3T3 cell lines: subcutaneous tumor cell take. J Natl Cancer Inst. 1981 Feb;66(2):303–310. [PubMed] [Google Scholar]