Skip to main content
NCBI home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1981 Nov;44(5):692–699. doi: 10.1038/bjc.1981.255

Electrophoretic mobility of mouse T-cell hybrids.

J Bubeník, P Perlmann, I Jónsdóttir, H Kypĕnová, D Bubeníková, J Símová
PMCID: PMC2010848  PMID: 6976180

Abstract

The hybrid cell line BH2 was derived by fusion between an AKR thymoma BW5147 (HGPRT-) and C57BL thymoma EL-4R (TK-). The hybrid cells showed a near-tetraploid modal number of chromosomes, in contrast to the near-diploid stem-lines of both parental cell populations; most of the BH2 hybrid cells acquired marker chromosomes from both parental cell lines. Inoculation of the parental and hybrid cells into C57BL and AKR mice revealed that the possible admixture of revertant parental cells in the hybrid cell population was less than 10(-4). Anodic electrophoretic mobilities (AEM) of the mouse thymoma lines and their hybrids were compared with each other and with normal mouse lymphoid cells. The AEM of the parental and hybrid T-cell lines was slower than that of mouse T LNC and comparable with AEM of some thymocyte subsets. The mean AEM of parental and hybrid cell lines was 0.69 micrometers/sec/V/cm for BW5147 cells, 0.96 for EL-4R cells and 0.83 for BH2 cells, the mean AEM of the hybrid cell population being identical with the mean of the parental AEM values. The mean AEM was found to be a relatively stable characteristic of each cell line.

Full text

PDF
692

Images in this article

695Fig. 1
on p.695

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bubeník J., Malkovský M., Suhajová E. The latex particle adherence (LPA) assay for detection of leukocytes with adhesive surface properties. Cell Immunol. 1978 Jan;35(1):217–225. doi: 10.1016/0008-8749(78)90141-7. [DOI] [PubMed] [Google Scholar]
  2. Donald D. C., Ho-Yen D. O., Hutchinson F., Macleod T. M. Electrophoretic mobility of lymphocyte subpopulations in infectious mononucleosis. Clin Exp Immunol. 1980 Apr;40(1):197–201. [PMC free article] [PubMed] [Google Scholar]
  3. Dumont F. Electrophoretic analysis of cell subpopulations in the mouse thymus as a function of age. Immunology. 1974 May;26(5):1051–1057. [PMC free article] [PubMed] [Google Scholar]
  4. Francke U., Gehring U. Chromosome assignment of a murine glucocorticoid receptor gene (Grl-1) using intraspecies somatic cell hybrids. Cell. 1980 Dec;22(3):657–664. doi: 10.1016/0092-8674(80)90541-3. [DOI] [PubMed] [Google Scholar]
  5. GORER P. A., AMOS D. B. Passive immunity in mice against C57BL leukosis E.L. 4 by means of iso-immune serum. Cancer Res. 1956 May;16(4):338–343. [PubMed] [Google Scholar]
  6. Jenkins R. Distribution of electrophoretic mobilities of mouse thymocyte subpopulations in the presence of tumour cells. Immunology. 1975 Nov;29(5):893–902. [PMC free article] [PubMed] [Google Scholar]
  7. LITTLEFIELD J. W. SELECTION OF HYBRIDS FROM MATINGS OF FIBROBLASTS IN VITRO AND THEIR PRESUMED RECOMBINANTS. Science. 1964 Aug 14;145(3633):709–710. doi: 10.1126/science.145.3633.709. [DOI] [PubMed] [Google Scholar]
  8. Malkovský M., Bubeník Human urinary bladder carcinoma cell line (T24) in long-term culture: chromosomal studies on a wild population and derived sublines. Neoplasma. 1977;24(3):319–326. [PubMed] [Google Scholar]
  9. Marikovsky Y., Ben-Bassat H., Leibovich S. J., Cividalli L., Fischler H., Danon D. Surface charge characteristics of cells from malignant cell lines and normal cell lines of the human hematopoietic system. J Natl Cancer Inst. 1979 Feb;62(2):285–292. [PubMed] [Google Scholar]
  10. Marshak-Rothstein A., Fink P., Gridley T., Raulet D. H., Bevan M. J., Gefter M. L. Properties and applications of monoclonal antibodies directed against determinants of the Thy-1 locus. J Immunol. 1979 Jun;122(6):2491–2497. [PubMed] [Google Scholar]
  11. Mohit B., Fan K. Hybrid cell line from a cloned immunoglobulin-producing mouse myeloma and a nonproducing mouse lymphoma. Science. 1971 Jan 8;171(3966):75–77. doi: 10.1126/science.171.3966.75. [DOI] [PubMed] [Google Scholar]
  12. Olive D., Sabolovic D., Beugnot M. C., Beaugnon M. Immunological characteristics of lymphoblasts and lymphocytes in acute lymphoblastic leukaemia in children. Clin Exp Immunol. 1977 Aug;29(2):220–228. [PMC free article] [PubMed] [Google Scholar]
  13. Rhie J. O., Sehon A. H. Specific alteration of the surface charge of immunocytes. Nat New Biol. 1972 Feb 2;235(57):156–158. doi: 10.1038/newbio235156a0. [DOI] [PubMed] [Google Scholar]
  14. Ruhenstroth-Bauer G., Lücke-Huhle C. Two populations of small lymphocytes. J Cell Biol. 1968 Apr;37(1):196–199. doi: 10.1083/jcb.37.1.196. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sabolovic D., Pompidou A., Amiel J. L. Blood lymphocytes in acute lymphoid leukaemia in remission and in relapse. Predictive value of electrophoretic mobility and refringence. Biomedicine. 1975 Sep 10;23(7):283–290. [PubMed] [Google Scholar]
  16. Sabolović D., Dumont F. Separation and characterization of cell subpopulations in the thymus. Immunology. 1973 Apr;24(4):601–606. [PMC free article] [PubMed] [Google Scholar]
  17. Suomalainen H. A., Goldsby R. A., Osborne B. A., Schröder J. Mouse/human T-cell hybrids rosetting with sheep erythrocytes. Scand J Immunol. 1980;11(2):163–168. doi: 10.1111/j.1365-3083.1980.tb00222.x. [DOI] [PubMed] [Google Scholar]
  18. Taniguchi M., Miller J. F. Specific suppressive factors produced by hybridomas derived from the fusion of enriched suppressor T cells and a T lymphoma cell line. J Exp Med. 1978 Aug 1;148(2):373–382. doi: 10.1084/jem.148.2.373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wiig J. N. Effect of neuraminidase on lymphoid cells. Differences in structure of B and T cells and thymocytes of the mouse shown by cell electrophoresis and sialic acid determination. Scand J Immunol. 1974;3(3):357–363. doi: 10.1111/j.1365-3083.1974.tb01265.x. [DOI] [PubMed] [Google Scholar]
  20. Wiig J. N. Electrophoresis of lymphoid cells. A study of Bruton type of agammaglobulinaemia, thymic dysplasia, chronic lymphatic leukaemia and of normal human thymocytes. Clin Exp Immunol. 1975 Jan;19(1):159–165. [PMC free article] [PubMed] [Google Scholar]
  21. Wiig J. N. Electrophoresis of lymphoid cells. Organ specific distribution pattern of small lymphoid cells in the mouse. Scand J Immunol. 1973;2(1):23–29. doi: 10.1111/j.1365-3083.1973.tb02012.x. [DOI] [PubMed] [Google Scholar]
  22. Wiig J. N. The effect of anti-lymphocyte serum, mitogens and enzymatic treatment on the agglutination and surface charge of lymphoid cells. Acta Pathol Microbiol Scand C. 1976 Jun;84(3):177–184. doi: 10.1111/j.1699-0463.1976.tb00017.x. [DOI] [PubMed] [Google Scholar]
  23. Wioland M., Sabolovic D., Burg C. Electrophoretic mobilities of T and B cells. Nat New Biol. 1972 Jun 28;237(78):274–276. doi: 10.1038/newbio237274a0. [DOI] [PubMed] [Google Scholar]
  24. Zeiller K., Holzberg E., Pascher G., Hannig K. Free flow electrophoretic separation of T and B lymphocytes. Evidence for various subpopulations of B cells. Hoppe Seylers Z Physiol Chem. 1972 Jan;353(1):105–110. [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES