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. 1981 Nov;44(5):675–681. doi: 10.1038/bjc.1981.253

Stimulation by CLL cells in mixed lymphocyte culture (MLC).

E H Jones, A B Hockley, S D Lawler
PMCID: PMC2010850  PMID: 6172142

Abstract

Cells from patients with chronic lymphocytic leukaemia (CLL) do not respond in the mixed lymphocyte culture (MLC) but are able to stimulate the response of normal lymphocytes. Mixed lymphocyte cultures were performed using cells from 24 patients with CLL and cells from 16 normal donors. The stimulatory capacity of 8 of these CLL cells was reduced when a common DR antigen was shared with the normal responding cell. We suggest that cells from certain selected CLL patients may be used in the mixed-lymphocyte reaction for determining the D-locus specificity of normal donors. CLL cells when expressed 1 DR antigen only, induced more clearly defined typing responses than cells with 2 DR antigens. There was no correlation between the ability of a CLL cell to induce a typing response and the T-cell status of the patient. However, a correlation with clinical course was suggested, because most cells which induced a typing response were obtained from patients who had received intensive treatment for the disease.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BAIN B., VAS M. R., LOWENSTEIN L. THE DEVELOPMENT OF LARGE IMMATURE MONONUCLEAR CELLS IN MIXED LEUKOCYTE CULTURES. Blood. 1964 Jan;23:108–116. [PubMed] [Google Scholar]
  2. Bach F. H., Amos D. B. Hu-1: Major histocompatibility locus in man. Science. 1967 Jun 16;156(3781):1506–1508. doi: 10.1126/science.156.3781.1506. [DOI] [PubMed] [Google Scholar]
  3. Catovsky D., Miliani E., Okos A., Galton D. A. Clinical significance of T-cells in chronic lymphocytic leukaemia. Lancet. 1974 Sep 28;2(7883):751–752. doi: 10.1016/s0140-6736(74)90944-1. [DOI] [PubMed] [Google Scholar]
  4. Godal T., Henriksen A., Iversen J. G., Landaas T. O., Lindmo T. Altered membrane-associated functions in chronic lymphocytic leukemia cells. Int J Cancer. 1978 May 15;21(5):561–569. doi: 10.1002/ijc.2910210505. [DOI] [PubMed] [Google Scholar]
  5. Halper J. P., Fu S. M., Gottlieb A. B., Winchester R. J., Kunkel H. G. Poor mixed lymphocyte reaction stimulatory capacity of leukemic B cells of chronic lymphocytic leukemia patients despite the presence of Ia antigens. J Clin Invest. 1979 Nov;64(5):1141–1148. doi: 10.1172/JCI109567. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kasakura S., Lowenstein L. A factor stimulating DNA synthesis derived from the medium of leukocyte cultures. Nature. 1965 Nov 20;208(5012):794–795. doi: 10.1038/208794a0. [DOI] [PubMed] [Google Scholar]
  7. Kasakura S. MLC stimulatory capacity and production of blastogenic factor in patients with chronic lymphatic leukemia and Hodgkin's disease. Blood. 1975 Jun;45(6):823–832. [PubMed] [Google Scholar]
  8. Lauria F., Foa R., Catovsky D. Increase in T gamma lymphocytes in B-cell chronic lymphocytic leukaemia. Scand J Haematol. 1980 Feb;24(2):187–190. doi: 10.1111/j.1600-0609.1980.tb02366.x. [DOI] [PubMed] [Google Scholar]
  9. Lawler S. D., Dewar P. J., Mrazek I., Jones E. H., Hockley A. B. Antisera with Ia specificity selected by lymphocytes from patients with chronic lymphocytic leukemia. Vox Sang. 1978;34(4):200–207. doi: 10.1111/j.1423-0410.1978.tb02468.x. [DOI] [PubMed] [Google Scholar]
  10. Mac Dermott R. P., Chess L., Schlossman S. F. Immunologic functions of isolated human lymphocyte subpopulations. V. Isolation and functional analysis of a surface Ig negative, E rosette negative subset. Clin Immunol Immunopathol. 1975 Sep;4(3):415–424. doi: 10.1016/0090-1229(75)90010-0. [DOI] [PubMed] [Google Scholar]
  11. McDevitt H. O., Chinitz A. Genetic control of the antibody response: relationship between immune response and histocompatibility (H-2) type. Science. 1969 Mar 14;163(3872):1207–1208. doi: 10.1126/science.163.3872.1207. [DOI] [PubMed] [Google Scholar]
  12. Moretta L., Mingari M. C., Webb S. R., Pearl E. R., Lydyard P. M., Grossi C. E., Lawton A. R., Cooper M. D. Imbalances in T cell subpopulations associated with immunodeficiency and autoimmune syndromes. Eur J Immunol. 1977 Oct;7(10):696–700. doi: 10.1002/eji.1830071009. [DOI] [PubMed] [Google Scholar]
  13. Pegrum G. D. Mixed culture of human foetal and adult cells. Immunology. 1971 Jul;21(1):159–164. [PMC free article] [PubMed] [Google Scholar]
  14. Rai K. R., Sawitsky A., Cronkite E. P., Chanana A. D., Levy R. N., Pasternack B. S. Clinical staging of chronic lymphocytic leukemia. Blood. 1975 Aug;46(2):219–234. [PubMed] [Google Scholar]
  15. Rudders R. A. B lymphocyte subpopulations in chronic lymphocytic leukemia. Blood. 1976 Feb;47(2):229–235. [PubMed] [Google Scholar]
  16. Rühl H., Vogt W., Bochert G., Schmidt S., Moelle R., Schaoua H. Mixed lymphocyte culture stimulatory and responding capacity of lymphocytes from patients with lymphoproliferative diseases. Clin Exp Immunol. 1975 Jan;19(1):55–65. [PMC free article] [PubMed] [Google Scholar]
  17. Wolos J. A., Davey F. R. Depressed stimulation in the MLR by B lymphocytes on chronic lymphocytic leukemia: failure to demonstrate a suppressor cell. Clin Immunol Immunopathol. 1979 Sep;14(1):77–85. doi: 10.1016/0090-1229(79)90128-4. [DOI] [PubMed] [Google Scholar]

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