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. 1982 Mar;45(3):456–465. doi: 10.1038/bjc.1982.74

Photodynamic action and chromosomal damage: a comparison of haematoporphyrin derivative (HpD) and light with X-irradiation.

J F Evensen, J Moan
PMCID: PMC2010917  PMID: 7073939

Abstract

Chromosomal aberrations (CA) are induced in human cells (NHIK 3025) in vitro when exposed to X-rays and to haematoporphyrin derivative (HpD) plus light. At the 0 . 1 survival level X-rays induce about 10 times more breaks per chromosome than the photodynamic treatment. There is some evidence for non-random distribution of the CA induced by HpD plus light; i.e. they seem to be localized at the centromeric and telomeric regions. Such non-random distribution of CA could be explained if centromeric and telomeric chromatin were associated with the inner nuclear membrane.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allison A. C., Paton G. R. Chromosome damage in human diploid cells following activation of lysosomal enzymes. Nature. 1965 Sep 11;207(5002):1170–1173. doi: 10.1038/2071170a0. [DOI] [PubMed] [Google Scholar]
  2. Buckton K. E. Identification with G and R banding of the position of breakage points induced in human chromosomes by in vitro x-irradiation. Int J Radiat Biol Relat Stud Phys Chem Med. 1976 May;29(5):475–488. doi: 10.1080/09553007614550571. [DOI] [PubMed] [Google Scholar]
  3. Carrano A. V. Chromosome aberrations and radiation-induced cell death. II. Predicted and observed cell survival. Mutat Res. 1973 Mar;17(3):355–366. doi: 10.1016/0027-5107(73)90007-9. [DOI] [PubMed] [Google Scholar]
  4. Christensen T., Moan J. Photodynamic inactivation of synchronized human cells in vitro in the presence of hematoporphyrin. Cancer Res. 1979 Sep;39(9):3735–3737. [PubMed] [Google Scholar]
  5. Dewey W. C., Miller H. H., Leeper D. B. Chromosomal aberrations and mortality of x-irradiated mammalian cells: emphasis on repair. Proc Natl Acad Sci U S A. 1971 Mar;68(3):667–671. doi: 10.1073/pnas.68.3.667. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Diamond I., Granelli S. G., McDonagh A. F., Nielsen S., Wilson C. B., Jaenicke R. Photodynamic therapy of malignant tumours. Lancet. 1972 Dec 2;2(7788):1175–1177. doi: 10.1016/s0140-6736(72)92596-2. [DOI] [PubMed] [Google Scholar]
  7. Dougherty T. J., Grindey G. B., Fiel R., Weishaupt K. R., Boyle D. G. Photoradiation therapy. II. Cure of animal tumors with hematoporphyrin and light. J Natl Cancer Inst. 1975 Jul;55(1):115–121. doi: 10.1093/jnci/55.1.115. [DOI] [PubMed] [Google Scholar]
  8. Dougherty T. J., Lawrence G., Kaufman J. H., Boyle D., Weishaupt K. R., Goldfarb A. Photoradiation in the treatment of recurrent breast carcinoma. J Natl Cancer Inst. 1979 Feb;62(2):231–237. [PubMed] [Google Scholar]
  9. Dougherty T. J., Thoma R. E., Boyle D. G., Weishaupt K. R. Interstitial photoradiation therapy for primary solid tumors in pet cats and dogs. Cancer Res. 1981 Feb;41(2):401–404. [PubMed] [Google Scholar]
  10. Evans H. J., O'Riordan M. L. Human peripheral blood lymphocytes for the analysis of chromosome aberrations in mutagen tests. Mutat Res. 1975 Jun;31(3):135–148. doi: 10.1016/0165-1161(75)90082-5. [DOI] [PubMed] [Google Scholar]
  11. Forbes I. J., Cowled P. A., Leong A. S., Ward A. D., Black R. B., Blake A. J., Jacka F. J. Phototherapy of human tumours using haematoporphyrin derivative. Med J Aust. 1980 Nov 1;2(9):489–493. doi: 10.5694/j.1326-5377.1980.tb100708.x. [DOI] [PubMed] [Google Scholar]
  12. Gomer C. J., Dougherty T. J. Determination of [3H]- and [14C]hematoporphyrin derivative distribution in malignant and normal tissue. Cancer Res. 1979 Jan;39(1):146–151. [PubMed] [Google Scholar]
  13. Gutter B., Speck W. T., Rosenkranz H. S. The photodynamic modification of DNA by hematoporphyrin. Biochim Biophys Acta. 1977 Mar 18;475(2):307–314. doi: 10.1016/0005-2787(77)90021-1. [DOI] [PubMed] [Google Scholar]
  14. Hsu T. C. A possible function of constitutive heterochromatin: the bodyguard hypothesis. Genetics. 1975 Jun;79 (Suppl):137–150. [PubMed] [Google Scholar]
  15. Ito T. Cellular and subcellular mechanisms of photodynamic action: the 1O2 hypothesis as a driving force in recent research. Photochem Photobiol. 1978 Oct-Nov;28(4-5):493–508. doi: 10.1111/j.1751-1097.1978.tb06957.x. [DOI] [PubMed] [Google Scholar]
  16. Jori G., Galiazzo G., Scoffone E. Photodynamic action of porphyrins on amino acids and proteins. I. Selective photooxidation of methionine in Aqueous solution. Biochemistry. 1969 Jul;8(7):2868–2875. doi: 10.1021/bi00835a026. [DOI] [PubMed] [Google Scholar]
  17. Kumar S., Natarajan A. T. Photodynamic action and post-irradiation modifying effects of methylene blue and acridine orange in barley and Vicia faba. Mutat Res. 1965 Feb;2(1):11–21. doi: 10.1016/0027-5107(65)90003-5. [DOI] [PubMed] [Google Scholar]
  18. LIPSON R. L., BALDES E. J., OLSEN A. M. The use of a derivative of hematoporhyrin in tumor detection. J Natl Cancer Inst. 1961 Jan;26:1–11. [PubMed] [Google Scholar]
  19. Lipson R. L., Baldes E. J., Gray M. J. Hematoporphyrin derivative for detection and management of cancer. Cancer. 1967 Dec;20(12):2255–2257. doi: 10.1002/1097-0142(196712)20:12<2255::aid-cncr2820201229>3.0.co;2-u. [DOI] [PubMed] [Google Scholar]
  20. Lloyd D. C., Purrott R. J., Dolphin G. W., Bolton D., Edwards A. A., Corp M. J. The relationship between chromosome aberrations and low LET radiation dose to human lymphocytes. Int J Radiat Biol Relat Stud Phys Chem Med. 1975 Jul;28(1):75–90. doi: 10.1080/09553007514550781. [DOI] [PubMed] [Google Scholar]
  21. Lücke-Huhle C. Biological relevance of alkali-labile sites in double-stranded DNA after gamma-irradiation. Int J Radiat Biol Relat Stud Phys Chem Med. 1975 Jan;27(1):1–6. doi: 10.1080/09553007514550011. [DOI] [PubMed] [Google Scholar]
  22. Moan J., Pettersen E. O., Christensen T. The mechanism of photodynamic inactivation of human cells in vitro in the presence of haematoporphyrin. Br J Cancer. 1979 Apr;39(4):398–407. doi: 10.1038/bjc.1979.72. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Moan J., Waksvik H., Christensen T. DNA single-strand breaks and sister chromatid exchanges induced by treatment with hematoporphyrin and light or by x-rays in human NHIK 3025 cells. Cancer Res. 1980 Aug;40(8 Pt 1):2915–2918. [PubMed] [Google Scholar]
  24. Profio A. E., Doiron D. R. A feasibility study of the use of fluorescence bronchoscopy for localization of small lung tumours. Phys Med Biol. 1977 Sep;22(5):949–957. doi: 10.1088/0031-9155/22/5/014. [DOI] [PubMed] [Google Scholar]
  25. Waksvik H., Brogger A., Stene J. Psoralen/UVA treatment and chromosomes. I. Aberrations and sister chromatid exchange in human lymphocytes in vitro and synergism with caffeine. Hum Genet. 1977 Sep 22;38(2):195–207. doi: 10.1007/BF00527403. [DOI] [PubMed] [Google Scholar]
  26. Weishaupt K. R., Gomer C. J., Dougherty T. J. Identification of singlet oxygen as the cytotoxic agent in photoinactivation of a murine tumor. Cancer Res. 1976 Jul;36(7 Pt 1):2326–2329. [PubMed] [Google Scholar]

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