Abstract
Human myeloma stem cells were detected by their capacity to form colonies in culture. Cells separated from aspirated marrow were cultured for 10 days in semi-solid methylcellulose with medium conditioned by T lymphocytes stimulated by phytohaemagglutinin (PHA-TCM). The colonies formed consisted mostly of lymphoplasmacytoid cells or plasma cells, and the immunoglobulins in the patients' myeloma cells were demonstrated also in the cytoplasm of the colony cells. The number of colonies were proportional to the number of cells plated and to the concentration of PHA-TCM. When the proportion of proliferating colony-forming units of multiple myeloma (CFU-MM) was studied using the (3H)-dT-suicide technique, the high-specific-activity (3H)-dT killed 21-45% of the CFU-MM in 7 myeloma patients. According to a single dose of Co-y-irradiation, the mean doses for impairment of regeneration (Do) were 1.00 and 1.63 Gy in 2 cases, the extrapolation numbers being 1.6 and 2.0.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Cronkite E. P., Feinendegen L. E. Notions about human stem cells. Nouv Rev Fr Hematol Blood Cells. 1976;17(1-2):269–284. doi: 10.1007/978-3-642-66312-3_18. [DOI] [PubMed] [Google Scholar]
- Fauser A. A., Messner H. A. Proliferative state of human pluripotent hemopoietic progenitors (CFU-GEMM) in normal individuals and under regenerative conditions after bone marrow transplantation. Blood. 1979 Nov;54(5):1197–1200. [PubMed] [Google Scholar]
- Iscove N. N. The role of erythropoietin in regulation of population size and cell cycling of early and late erythroid precursors in mouse bone marrow. Cell Tissue Kinet. 1977 Jul;10(4):323–334. doi: 10.1111/j.1365-2184.1977.tb00300.x. [DOI] [PubMed] [Google Scholar]
- Izaguirre C. A., Minden M. D., Howatson A. F., McCulloch E. A. Colony formation by normal and malignant human B-lymphocytes. Br J Cancer. 1980 Sep;42(3):430–437. doi: 10.1038/bjc.1980.255. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Minden M. D., Buick R. N., McCulloch E. A. Separation of blast cell and T-lymphocyte progenitors in the blood of patients with acute myeloblastic leukemia. Blood. 1979 Jul;54(1):186–195. [PubMed] [Google Scholar]