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. 1982 Aug;46(2):266–278. doi: 10.1038/bjc.1982.192

Correlation of collagenase secretion with metastatic-colonization potential in naturally occurring murine mammary tumours.

D Tarin, B J Hoyt, D J Evans
PMCID: PMC2011086  PMID: 6295423

Abstract

We report evidence for the secretion of a true mammalian collagenase active against Type 1 collagen, by naturally-occurring mammary tumours of the mouse and show that tumours capable of heavily colonizing the lungs secrete significantly more of this enzyme than those with low pulmonary-colonization potential, or non-neoplastic proliferating (e.g. lactating) mammary tissue. Plasminogen activator is secreted in greater quantity by tumours than by normal tissues, but there is no significant difference in the amount produced by tumours with high or low pulmonary-colonization potential. These findings correlate well with our earlier morphological observations of marked connective tissue destruction in the vicinity of invading tumours and metastatic deposits, and indicate that protease release is implicated in the mechanism of tumour spread.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abramson M., Schiling R. W., Huang C. C., Salome R. G. Collagenase activity in epidermoid carcinoma of the oral cavity and larynx. Ann Otol Rhinol Laryngol. 1975 Mar-Apr;84(2 Pt 1):158–163. doi: 10.1177/000348947508400203. [DOI] [PubMed] [Google Scholar]
  2. Dabbous M. K., Roberts A. N., Brinkley B. Collagenase and neutral protease activities in cultures of rabbit VX-2 carcinoma. Cancer Res. 1977 Oct;37(10):3537–3544. [PubMed] [Google Scholar]
  3. Eisen A. Z., Jeffrey J. J., Gross J. Human skin collagenase. Isolation and mechanism of attack on the collagen molecule. Biochim Biophys Acta. 1968 Mar 25;151(3):637–645. doi: 10.1016/0005-2744(68)90010-7. [DOI] [PubMed] [Google Scholar]
  4. GROSS J. Studies on the formation of collagen. I. Properties and fractionation of neutral salt extracts of normal guinea pig connective tissue. J Exp Med. 1958 Feb 1;107(2):247–263. doi: 10.1084/jem.107.2.247. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gisslow M. T., McBride B. C. A rapid sensitive collagenase assay. Anal Biochem. 1975 Sep;68(1):70–78. doi: 10.1016/0003-2697(75)90680-6. [DOI] [PubMed] [Google Scholar]
  6. Hashimoto K., Yamanishi Y., Maeyens E., Dabbous M. K., Kanzaki T. Collagenolytic activities of squamous cell carcinoma of the skin. Cancer Res. 1973 Nov;33(11):2790–2801. [PubMed] [Google Scholar]
  7. Jones P. A., Laug W. E., Benedict W. F. Fibrinolytic activity in a human fibrosarcoma cell line and evidence for the induction of plasminogen activator secretion during tumor formation. Cell. 1975 Oct;6(2):245–252. doi: 10.1016/0092-8674(75)90015-x. [DOI] [PubMed] [Google Scholar]
  8. Kuettner K. E., Soble L., Croxen R. L., Marczynska B., Hiti J., Harper E. Tumor cell collagenase and its inhibition by a cartilage-derived protease inhibitor. Science. 1977 May 6;196(4290):653–654. doi: 10.1126/science.193181. [DOI] [PubMed] [Google Scholar]
  9. Liotta L. A., Abe S., Robey P. G., Martin G. R. Preferential digestion of basement membrane collagen by an enzyme derived from a metastatic murine tumor. Proc Natl Acad Sci U S A. 1979 May;76(5):2268–2272. doi: 10.1073/pnas.76.5.2268. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Liotta L. A., Tryggvason K., Garbisa S., Hart I., Foltz C. M., Shafie S. Metastatic potential correlates with enzymatic degradation of basement membrane collagen. Nature. 1980 Mar 6;284(5751):67–68. doi: 10.1038/284067a0. [DOI] [PubMed] [Google Scholar]
  11. Markus G., Takita H., Camiolo S. M., Corasanti J. G., Evers J. L., Hobika G. H. Content and characterization of plasminogen activators in human lung tumors and normal lung tissue. Cancer Res. 1980 Mar;40(3):841–848. [PubMed] [Google Scholar]
  12. Nagy B., Ban J., Brdar B. Fibrinolysis associated with human neoplasia: production of plasminogen activator by human tumours. Int J Cancer. 1977 May 15;19(5):614–620. doi: 10.1002/ijc.2910190504. [DOI] [PubMed] [Google Scholar]
  13. Rifkin D. B., Loeb J. N., Moore G., Reich E. Properties of plasminogen activators formed by neoplastic human cell cultures. J Exp Med. 1974 May 1;139(5):1317–1328. doi: 10.1084/jem.139.5.1317. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sakai T., Gross J. Some properties of the products of reaction of tadpole collagenase with collagen. Biochemistry. 1967 Feb;6(2):518–528. doi: 10.1021/bi00854a021. [DOI] [PubMed] [Google Scholar]
  15. Stricklin G. P., Bauer E. A., Jeffrey J. J., Eisen A. Z. Human skin collagenase: isolation of precursor and active forms from both fibroblast and organ cultures. Biochemistry. 1977 Apr 19;16(8):1607–1615. doi: 10.1021/bi00627a013. [DOI] [PubMed] [Google Scholar]
  16. Stricklin G. P., Eisen A. Z., Bauer E. A., Jeffrey J. J. Human skin fibroblast collagenase: chemical properties of precursor and active forms. Biochemistry. 1978 Jun 13;17(12):2331–2337. doi: 10.1021/bi00605a012. [DOI] [PubMed] [Google Scholar]
  17. Tarin D. Fine structure of murine mammary tumours: the relationship between epithelium and connective tissue in neoplasms induced by various agents. Br J Cancer. 1969 Jun;23(2):417–425. doi: 10.1038/bjc.1969.54. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Tarin D., Price J. E. Metastatic colonization potential of primary tumour cells in mice. Br J Cancer. 1979 Jun;39(6):740–754. doi: 10.1038/bjc.1979.128. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Tarin D. Sequential electron microscopical study of experimental mouse skin carcinogenesis. Int J Cancer. 1967 May 15;2(3):195–211. doi: 10.1002/ijc.2910020302. [DOI] [PubMed] [Google Scholar]
  20. Tucker W. S., Kirsch W. M., Martinez-Hernandez A., Fink L. M. In vitro plasminogen activator activity in human brain tumors. Cancer Res. 1978 Feb;38(2):297–302. [PubMed] [Google Scholar]
  21. Wang B. S., McLoughlin G. A., Richie J. P., Mannick J. A. Correlation of the production of plasminogen activator with tumor metastasis in B16 mouse melanoma cell lines. Cancer Res. 1980 Feb;40(2):288–292. [PubMed] [Google Scholar]
  22. Wirl G., Frick J. Collagenase--a marker enzyme in human bladder cancer? Urol Res. 1979 Jun 22;7(2):103–108. doi: 10.1007/BF00254689. [DOI] [PubMed] [Google Scholar]
  23. Woolley D. E., Glanville R. W., Roberts D. R., Evanson J. M. Purification, characterization and inhibition of human skin collagenase. Biochem J. 1978 Feb 1;169(2):265–276. doi: 10.1042/bj1690265. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Woolley D. E., Grafton C. A. Collagenase immunolocalization studies of cutaneous secondary melanomas. Br J Cancer. 1980 Aug;42(2):260–265. doi: 10.1038/bjc.1980.225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Yamanishi Y., Maeyens E., Kabbous M. K., Ohyama H., Hashimoto K. Collagenolytic activity in malignant melanoma: physiocochemical studies. Cancer Res. 1973 Oct;33(10):2507–2512. [PubMed] [Google Scholar]

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