Skip to main content
NCBI home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1982 Aug;46(2):190–197. doi: 10.1038/bjc.1982.183

Syngeneic antitumour antibodies in rats: clearance of cell-bound antibody in vivo and in vitro.

C J Dean, S M Hobbs, J U Hopkins, S M North, J M Styles
PMCID: PMC2011097  PMID: 7150472

Abstract

Hooded Lister/Cbi rats bearing the HSN.TC fibrosarcoma produced a high-titre non-complement-binding IgG antibody, and tests in vitro indicated that the syngeneic antibody was specific for this tumour. About 1.4 X 10(5) antibody molecules were bound per cell, a figure one eighth that for cells treated with a high-titre allo-antiserum. When tumour-bearer serum was passively transferred into congenitally athymic rats bearing the HSN.TC tumour the antibody was absorbed out specifically, by comparison with control animals or athymic rats bearing an unrelated tumour that was also syngeneic in Hooded rats. The kinetics of loss of antibody from the surface of HSN.TC cells has been monitored in vitro and the antibody has been found to have an extended half-life at the cell surface (greater than 40 h).

Full text

PDF
190

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Currie G. A., Gage J. O. Influence of tumour growth on the evolution of cytotoxic lymphoid cells in rats bearing a spontaneously metastasizing syngeneic fibrosarcoma. Br J Cancer. 1973 Aug;28(2):136–146. doi: 10.1038/bjc.1973.131. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Eccles S. A., Alexander P. Macrophage content of tumours in relation to metastatic spread and host immune reaction. Nature. 1974 Aug 23;250(5468):667–669. doi: 10.1038/250667a0. [DOI] [PubMed] [Google Scholar]
  3. Eccles S. A., Heckford S. E., Alexander P. Effect of cyclosporin A on the growth and spontaneous metastasis of syngeneic animal tumours. Br J Cancer. 1980 Aug;42(2):252–259. doi: 10.1038/bjc.1980.224. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Eccles S. A., Styles J. M., Hobbs S. M., Dean C. J. Metastasis in the nude rat associated with lack of immune response. Br J Cancer. 1979 Nov;40(5):802–805. doi: 10.1038/bjc.1979.264. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gyure L. A., Dean C. J., Hall J. G., Styles J. M. Tumour-specific antibodies of the IgA class in rats after the implantation of a syngeneic tumour in the gut. Br J Cancer. 1980 Apr;41(4):640–641. doi: 10.1038/bjc.1980.108. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Muller W. A., Steinman R. M., Cohn Z. A. The membrane proteins of the vacuolar system. II. Bidirectional flow between secondary lysosomes and plasma membrane. J Cell Biol. 1980 Jul;86(1):304–314. doi: 10.1083/jcb.86.1.304. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Old L. J., Stockert E., Boyse E. A., Kim J. H. Antigenic modulation. Loss of TL antigen from cells exposed to TL antibody. Study of the phenomenon in vitro. J Exp Med. 1968 Mar 1;127(3):523–539. doi: 10.1084/jem.127.3.523. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Schneider Y. J., Tulkens P., de Duve C., Trouet A. Fate of plasma membrane during endocytosis. II. Evidence for recycling (shuttle) of plasma membrane constituents. J Cell Biol. 1979 Aug;82(2):466–474. doi: 10.1083/jcb.82.2.466. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Shepherd P. S., Dean C. J. The use of [125I]C1q subcomponent for the measurement of complement binding antibodies on cell surfaces. J Immunol Methods. 1979;25(1):55–60. doi: 10.1016/0022-1759(79)90165-0. [DOI] [PubMed] [Google Scholar]
  10. Taylor R. B., Clarke L. J., Elson C. J. Low avidity as a cause of prozone phenomena. J Immunol Methods. 1979;26(1):25–37. doi: 10.1016/0022-1759(79)90038-3. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES