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. 1982 Oct;46(4):611–616. doi: 10.1038/bjc.1982.245

Natural killer (NK) activity in peripheral blood lymphocytes of patients with benign and malignant breast disease

D White, D B Jones, T Cooke, N Kirkham
PMCID: PMC2011176  PMID: 7138766

Abstract

Previous studies of natural killer (NK) activity in the peripheral blood of breast cancer patients have failed to show a reduction in cytotoxicity, an observation at variance with results obtained in other malignancies. Interpretation of the data however is complicated by the presence of treated and post-mastectomy patients in the groups studied.

In this study, lymphocytes from preoperative blood samples of untreated women with benign and malignant breast disease were tested at various effector-to-target ratios for cytotoxicity activity against the NK sensitive erythromyeloid cell line, K562.

A significant reduction in NK activity was observed between carcinoma patients and the control group (P=0·02). When the carcinoma group was further divided into pre- and postmenopausal patients, the reduction was found to be a feature only of premenopausal women (P=0·002). The levels of NK activity in patients with benign breast disease were not significantly different from those in controls, irrespective of menstrual status. There was no correlation between NK activity and tumour size, oestrogen-receptor or lymph-node status in the carcinoma patients.

A preliminary analysis of NK activities in the control group suggests that women donating blood in the first half of their menstrual cycle show significantly reduced NK activity in comparison with those in the second half (P=0·001). This finding, coupled with the variation in NK activity shown between pre- and postmenopausal breast carcinoma patients, suggests that hormonal effects in conjunction with malignancy determine the level of NK activity in breast cancer.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baines M. G., Pross H. F., Millar K. G. Spontaneous human lymphocyte-mediated cytotoxicity against tumor target cells. IV. The suppressive effect of normal pregnancy. Am J Obstet Gynecol. 1978 Apr 1;130(7):741–744. doi: 10.1016/0002-9378(78)90001-7. [DOI] [PubMed] [Google Scholar]
  2. Cunningham-Rundles S., Filippa D. A., Braun D. W., Jr, Antonelli P., Ashikari H. Natural cytotoxicity of peripheral blood lymphocytes and regional lymph node cells in breast cancer in women. J Natl Cancer Inst. 1981 Sep;67(3):585–590. [PubMed] [Google Scholar]
  3. Einhorn S., Blomgren H., Strander H. Interferon and spontaneous cytotoxicity in man. I. Enhancement of the spontaneous cytotoxicity of peripheral lymphocytes by human leukocyte interferon. Int J Cancer. 1978 Oct 15;22(4):405–412. doi: 10.1002/ijc.2910220407. [DOI] [PubMed] [Google Scholar]
  4. Eremin O., Ashby J., Stephens J. P. Human natural cytotoxicity in the blood and lymphoid organs of healthy donors and patients with malignant disease. Int J Cancer. 1978 Jan 15;21(1):35–41. doi: 10.1002/ijc.2910210108. [DOI] [PubMed] [Google Scholar]
  5. GRATTAROLA R. THE PREMENSTRUAL ENDOMETRIAL PATTERN OF WOMEN WITH BREAST CANCER. A STUDY OF PROGESTATIONAL ACTIVITY. Cancer. 1964 Sep;17:1119–1122. doi: 10.1002/1097-0142(196409)17:9<1119::aid-cncr2820170904>3.0.co;2-1. [DOI] [PubMed] [Google Scholar]
  6. Hanna N. Expression of metastatic potential of tumor cells in young nude mice is correlated with low levels of natural killer cell-mediated cytotoxicity. Int J Cancer. 1980 Nov 15;26(5):675–680. doi: 10.1002/ijc.2910260521. [DOI] [PubMed] [Google Scholar]
  7. Herberman R. B., Holden H. T. Natural cell-mediated immunity. Adv Cancer Res. 1978;27:305–377. doi: 10.1016/s0065-230x(08)60936-7. [DOI] [PubMed] [Google Scholar]
  8. Hersey P., Edwards A., Honeyman M., McCarthy W. H. Low natural-killer-cell activity in familial melanoma patients and their relatives. Br J Cancer. 1979 Jul;40(1):113–122. doi: 10.1038/bjc.1979.147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Johnsen H. E., Madsen M. Lymphocyte subpopulations in man: characterization of in vivo-educated, alloreactive, cytotoxic lymphocytes. Acta Pathol Microbiol Scand C. 1980 Jun;88(3):163–171. doi: 10.1111/j.1699-0463.1980.tb00090.x. [DOI] [PubMed] [Google Scholar]
  10. Kalland T. Reduced natural killer activity in female mice after neonatal exposure to diethylstilbestrol. J Immunol. 1980 Mar;124(3):1297–1300. [PubMed] [Google Scholar]
  11. Kovithavongs T., Hottman W. C., Dossetor J. B. Effector cell activity in antibody-mediated cell-dependent immune lympholysis. I. Normal individuals. J Immunol. 1974 Oct;113(4):1178–1183. [PubMed] [Google Scholar]
  12. Lozzio C. B., Lozzio B. B., Yang W. K., Ichiki A. T., Bamberger E. G. Absence of thymus-derived lymphocyte markers in myelogenous leukemia (Ph1+) cell line K-562. Cancer Res. 1976 Dec;36(12):4657–4662. [PubMed] [Google Scholar]
  13. Menon M., Stefani S. S. Lymphocyte mediated natural cytotoxicity in neoplasia. Oncology. 1978;35(2):63–67. doi: 10.1159/000225257. [DOI] [PubMed] [Google Scholar]
  14. Moore M., Potter M. R. Enhancement of human natural cell-mediated cytotoxicity by interferon. Br J Cancer. 1980 Mar;41(3):378–387. doi: 10.1038/bjc.1980.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Payne S. V., Jones D. B., Haegert D. G., Smith J. L., Wright D. H. T and B lymphocytes and Reed-Sternberg cells in Hodgkin's disease lymph nodes and spleens. Clin Exp Immunol. 1976 May;24(2):280–286. [PMC free article] [PubMed] [Google Scholar]
  16. Pross H. F., Baines M. G. Spontaneous human lymphocyte-mediated cytotoxicity againts tumour target cells. I. The effect of malignant disease. Int J Cancer. 1976 Nov 15;18(5):593–604. doi: 10.1002/ijc.2910180508. [DOI] [PubMed] [Google Scholar]
  17. Seaman W. E., Blackman M. A., Gindhart T. D., Roubinian J. R., Loeb J. M., Talal N. beta-Estradiol reduces natural killer cells in mice. J Immunol. 1978 Dec;121(6):2193–2198. [PubMed] [Google Scholar]
  18. Seaman W. E., Gindhart T. D., Greenspan J. S., Blackman M. A., Talal N. Natural killer cells, bone, and the bone marrow: studies in estrogen-treated mice and in congenitally osteopetrotic (mi/mi) mice. J Immunol. 1979 Jun;122(6):2541–2547. [PubMed] [Google Scholar]
  19. Seaman W. E., Merigan T. C., Talal N. Natural killing in estrogen-treated mice responds poorly to poly I.C despite normal stimulation of circulating interferon. J Immunol. 1979 Dec;123(6):2903–2905. [PubMed] [Google Scholar]
  20. Takasugi M., Ramseyer A., Takasugi J. Decline of natural nonselective cell-mediated cytotoxicity in patients with tumor progression. Cancer Res. 1977 Feb;37(2):413–418. [PubMed] [Google Scholar]
  21. Trinchieri G., Santoli D., Koprowski H. Spontaneous cell-mediated cytotoxicity in humans: role of interferon and immunoglobulins. J Immunol. 1978 Jun;120(6):1849–1855. [PubMed] [Google Scholar]
  22. Yam L. T., Li C. Y., Crosby W. H. Cytochemical identification of monocytes and granulocytes. Am J Clin Pathol. 1971 Mar;55(3):283–290. doi: 10.1093/ajcp/55.3.283. [DOI] [PubMed] [Google Scholar]

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