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. 1982 Oct;46(4):568–581. doi: 10.1038/bjc.1982.241

Studies of lectin binding to normal and neoplastic lymphoid tissues. I. Normal nodes and Hodgkin's disease.

V H Bramwell, D Crowther, J Gallagher, R W Stoddart
PMCID: PMC2011180  PMID: 6182896

Abstract

Lectins are proteins which have the ability to interact specifically with carbohydrate residues of glycoproteins and other glycoconjugates. The staining patterns of 10 fluorescein conjugated lectins (F-Con A, F-LCA, F-RCA, F-WGA, F-PHA, F-PWM, F-LTA, F-SBA, F-PNA, F-DB) and a protease inhibitor (F-LA) have been studied in histological sections of 11 normal or reactive lymph nodes and 6 nodes and one skin biopsy involved by Hodgkin's disease. On the basis of the patterns of lectin binding, and current knowledge of their saccharide specificities, we found that within germinal centres there is an orderly carbohydrate rich extracellular matrix which contains a higher concentration of GlcNAc and terminal Gal residues than the surface membranes of component cells. This suggests active secretion rather than simple membrane shedding, and it is possible that this pericellular domain plays a part in the regulation of the proliferative response, or controls migration of lymphocytes in and out of the germinal centre. Lectin binding in Reed-Sternberg cells suggests that the huge nucleoli contain glycoconjugates of diverse structure, which may be linked with their failure to undergo cytokinesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Archibald R. B., Frenster J. H. Quantitative ultrastructural analysis of in vivo lymphocyte-Reed-Sternberg cell interactions in Hodgkin's disease. Natl Cancer Inst Monogr. 1973 May;36:239–245. [PubMed] [Google Scholar]
  2. Bhavanandan V. P., Katlic A. W. The interaction of wheat germ agglutinin with sialoglycoproteins. The role of sialic acid. J Biol Chem. 1979 May 25;254(10):4000–4008. [PubMed] [Google Scholar]
  3. Braylan R. C., Fowlkes B. J., Jaffe E. S., Sanders S. K., Berard C. W., Herman C. J. Cell volumes and DNA distributions of normal and neoplastic human lymphoid cells. Cancer. 1978 Jan;41(1):201–209. doi: 10.1002/1097-0142(197801)41:1<201::aid-cncr2820410129>3.0.co;2-6. [DOI] [PubMed] [Google Scholar]
  4. Braylan R. C., Jaffe E. S., Berard C. W. Letter: Surface characteristics of Hodgkin's lymphoma cells. Lancet. 1974 Nov 30;2(7892):1328–1329. doi: 10.1016/s0140-6736(74)90198-6. [DOI] [PubMed] [Google Scholar]
  5. Braylan R. C., Rappaport H. Tissue immunoglobulins in nodular lymphomas as compared with reactive follicular hyperplasias. Blood. 1973 Oct;42(4):579–589. [PubMed] [Google Scholar]
  6. Carr I. The ultrastructure of the abnormal reticulum cells in Hodgkin's disease. J Pathol. 1975 Jan;115(1):45–50. doi: 10.1002/path.1711150107. [DOI] [PubMed] [Google Scholar]
  7. Coppleson L. W., Rappaport H., Strum S. B., Rose J. Analysis of the Rye classification of Hodgkin's disease. The prognostic significance of cellular composition. J Natl Cancer Inst. 1973 Aug;51(2):379–390. [PubMed] [Google Scholar]
  8. Dorfman R. F., Rice D. F., Mitchell A. D., Kempson R. L., Levine G. Ultrastructural studies of Hodgkin's disease. Natl Cancer Inst Monogr. 1973 May;36:221–238. [PubMed] [Google Scholar]
  9. Essner E., Schreiber J., Griewski R. A. Localization of carbohydrate components in rat colon with fluoresceinated lectins. J Histochem Cytochem. 1978 Jun;26(6):452–458. doi: 10.1177/26.6.670681. [DOI] [PubMed] [Google Scholar]
  10. Etzler M. E., Branstrator M. L. Differential localization of cell surface and secretory components in rat intestinal epithelium by use of lectins. J Cell Biol. 1974 Aug;62(2):329–343. doi: 10.1083/jcb.62.2.329. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Furcht L. T., Mosher D. F., Wendelschafer-Crabb G. Effects of cell density and transformation on the formation of a fibronectin extracellular filamentous matrix on human fibroblasts. Cancer Res. 1978 Dec;38(12):4618–4623. [PubMed] [Google Scholar]
  12. Garvin A. J., Spicer S. S., Parmley R. T., Munster A. M. Immunohistochemical demonstration of IgG in Reed-Sternberg and other cells in Hodgkin's disease. J Exp Med. 1974 May 1;139(5):1077–1083. doi: 10.1084/jem.139.5.1077. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hayhoe F. G., Burns G. F., Cawley J. C., Stewart J. W. Cytochemical, ultrastructural and immunological studies of circulating Reed-Sternberg cells. Br J Haematol. 1978 Apr;38(4):485–490. doi: 10.1111/j.1365-2141.1978.tb01073.x. [DOI] [PubMed] [Google Scholar]
  14. Jacobson W., Stoddart R. W., Collins R. D. Lectin staining of carbohydrates of haemic cells. II. The cells of normal lymphoid origin, of lymphatic leukaemias and related diseases. Histopathology. 1980 Sep;4(5):491–500. doi: 10.1111/j.1365-2559.1980.tb02944.x. [DOI] [PubMed] [Google Scholar]
  15. Janossy G., Thomas J. A., Pizzolo G., Granger S. M., McLaughlin J., Habeshaw J. A., Stansfeld A. G., Sloane J. Immuno-histological diagnosis of lymphoproliferative diseases by selected combinations of antisera and monoclonal antibodies. Br J Cancer. 1980 Aug;42(2):224–242. doi: 10.1038/bjc.1980.222. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kadin M. E., Stites D. P., Levy R., Warnke R. Exogenous immunoglobulin and the macrophage origin of Reed-Sternberg cells in Hodgkin's disease. N Engl J Med. 1978 Nov 30;299(22):1208–1214. doi: 10.1056/NEJM197811302992203. [DOI] [PubMed] [Google Scholar]
  17. Kaplan H. S., Gartner S. "Sternberg-reed" giant cells of Hodgkin's Disease: cultivation in vitro, heterotransplantation, and characterization as neoplastic macrophages. Int J Cancer. 1977 Apr 15;19(4):511–525. doi: 10.1002/ijc.2910190412. [DOI] [PubMed] [Google Scholar]
  18. Kiernan J. A., Stoddart R. W. Fluorescent-labelled aprotinin: a new reagent for the histochemical detection of acid mucosubstances. Histochemie. 1973;34(1):77–84. doi: 10.1007/BF00304309. [DOI] [PubMed] [Google Scholar]
  19. Kornfeld K., Reitman M. L., Kornfeld R. The carbohydrate-binding specificity of pea and lentil lectins. Fucose is an important determinant. J Biol Chem. 1981 Jul 10;256(13):6633–6640. [PubMed] [Google Scholar]
  20. Leech J. Immunoglobulin-positive Reed Sternberg cells in Hodgkin's disease. Lancet. 1973 Aug 4;2(7823):265–266. doi: 10.1016/s0140-6736(73)93173-5. [DOI] [PubMed] [Google Scholar]
  21. Narasimhan S., Wilson J. R., Martin E., Schachter H. A structural basis for four distinct elution profiles on concanavalin A--Sepharose affinity chromatography of glycopeptides. Can J Biochem. 1979 Jan;57(1):83–96. doi: 10.1139/o79-011. [DOI] [PubMed] [Google Scholar]
  22. Nieland M. L. Epidermal intercellular staining with fluorescein-conjugated phytohemagglutinins. J Invest Dermatol. 1973 Feb;60(2):61–66. doi: 10.1111/1523-1747.ep12724137. [DOI] [PubMed] [Google Scholar]
  23. Parodi A. J., Leloir L. F. The role of lipid intermediates in the glycosylation of proteins in the eucaryotic cell. Biochim Biophys Acta. 1979 Apr 23;559(1):1–37. doi: 10.1016/0304-4157(79)90006-6. [DOI] [PubMed] [Google Scholar]
  24. Payne S. V., Jones D. B., Haegert D. G., Smith J. L., Wright D. H. T and B lymphocytes and Reed-Sternberg cells in Hodgkin's disease lymph nodes and spleens. Clin Exp Immunol. 1976 May;24(2):280–286. [PMC free article] [PubMed] [Google Scholar]
  25. Payne S. V., Jones D. B., Wright D. H. Reed-Sternberg-cell/lymphocyte interaction. Lancet. 1977 Oct 8;2(8041):768–769. doi: 10.1016/s0140-6736(77)90281-1. [DOI] [PubMed] [Google Scholar]
  26. Roth J., Binder M., Gerhard U. J. Conjugation of lectins with fluorochromes: an approach to histochemical double labeling of carbohydrate components. Histochemistry. 1978 Jul 12;56(3-4):265–273. doi: 10.1007/BF00495988. [DOI] [PubMed] [Google Scholar]
  27. Stoddart R. W., Collins R. D., Jacobson W. Lectin staining of carbohydrates of haemic cells; the cells of normal blood and bone marrow and of the myeloid leukaemias. J Pathol. 1980 Aug;131(4):321–332. doi: 10.1002/path.1711310404. [DOI] [PubMed] [Google Scholar]
  28. Stoddart R. W., Herbertson B. M. The use of fluorescein-labelled lectins in the detection and identification of fungi pathogenic for man: a preliminary study. J Med Microbiol. 1978 Aug;11(3):315–324. doi: 10.1099/00222615-11-3-315. [DOI] [PubMed] [Google Scholar]
  29. Stoddart R. W., Herbertson B. M. The use of lectins in the detection and identification of human fungal pathogens. Biochem Soc Trans. 1977;5(1):233–235. doi: 10.1042/bst0050233. [DOI] [PubMed] [Google Scholar]
  30. Stoddart R. W., Kernan J. A. Aprotinin, a carbohydrate-binding protein. Histochemie. 1973 Mar 26;34(4):275–280. doi: 10.1007/BF00306299. [DOI] [PubMed] [Google Scholar]
  31. Stoddart R. W., Kiernan J. A. Histochemical detection of the -D-arabinopyranoside configuration using fluorescent-labelled concanavalin A. Histochemie. 1973;33(1):87–94. doi: 10.1007/BF00304230. [DOI] [PubMed] [Google Scholar]
  32. Stuart A. E., Williams A. R., Habeshaw J. A. Rosetting and other reactions of the Reed-Sternberg cell. J Pathol. 1977 Jun;122(2):81–90. doi: 10.1002/path.1711220205. [DOI] [PubMed] [Google Scholar]
  33. Sutcliffe S. B., Wrigley P. F., Peto J., Lister T. A., Stansfeld A. G., Whitehouse J. M., Crowther D., Malpas J. S. MVPP chemotherapy regimen for advanced Hodgkin's disease. Br Med J. 1978 Mar 18;1(6114):679–683. doi: 10.1136/bmj.1.6114.679. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Whyte A., Loke Y. W., Stoddart R. W. Saccharide distribution in human trophoblast demonstrated using fluorescein-labelled lectins. Histochem J. 1978 Jul;10(4):417–423. doi: 10.1007/BF01003005. [DOI] [PubMed] [Google Scholar]

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