Skip to main content
NCBI home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1982 Oct;46(4):582–592. doi: 10.1038/bjc.1982.242

Studies of lectin binding to normal and neoplastic lymph nodes. II. Non-Hodgkin's lymphoma.

V H Bramwell, D Crowther, J Gallagher, R W Stoddart
PMCID: PMC2011191  PMID: 6182897

Abstract

Fluorescein conjugated lectins have been used as histochemical stains in lymph node sections from 22 patients with non-Hodgkin's Lymphoma. Variations in the distribution and structure of glycoprotein sequences between the different types of lymphoma, and also normal nodes, have been detected. The lectin-binding patterns of neoplastic lymphocytes of small cell lymphomas, both follicular and diffuse, suggested a predominance of sialylated glycopeptides, as in normal small lymphocytes of the mantle zone of germinal centres. In contrast, the staining patterns of large cell follicular and diffuse lymphomas showed a greater diversity of carbohydrate structure, with enhanced cytoplasmic staining and increased numbers of incomplete oligosaccharide sequences. Heterogeneity of staining, together with reduced sialic acid expression at all cellular sites was a common feature of lymphoblastic lymphomas and seemed to be linked with a poor prognosis. The extracellular matrix of small cell follicular lymphomas showed altered saccharide content, but retained some degree of organization. The large cell follicular lymphomas were characterized by a prominent disorderly matrix, with staining characteristics which suggested shedding of surface membrane from component cells. The loss or disordered production of the normal extracellular matrix may reflect a breakdown of control mechanisms within neoplastic follicles.

Full text

PDF
582

Images in this article

584Fig. 1
on p.584
587Fig. 2
on p.587
588Fig. 3
on p.588
589Fig. 4
on p.589

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Atkinson M. A., Bramwell M. E. Studies on the surface properties of hybrid cells. I. Sialyl-transferase activity in homogenates of malignant and non-malignant cells. J Cell Sci. 1980 Dec;46:187–201. doi: 10.1242/jcs.46.1.187. [DOI] [PubMed] [Google Scholar]
  2. Atkinson M. A., Bramwell M. E. Studies on the surface properties of hybrid cells. II. Sialyl-transferase activity on the surface of malignant and non-malignant cells. J Cell Sci. 1980 Dec;46:203–220. doi: 10.1242/jcs.46.1.203. [DOI] [PubMed] [Google Scholar]
  3. Berard C. W., Cossman J., Jaffe E. S. Malignant lymphomas as tumours of the immune system. Br J Cancer. 1980 Jul;42(1):1–20. doi: 10.1038/bjc.1980.197. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Berard C. W., Jaffe E. S., Braylan R. C., Mann R. B., Nanba K. Immunologic aspects and pathology of the malignant lymphomas. Cancer. 1978 Aug;42(2 Suppl):911–921. doi: 10.1002/1097-0142(197808)42:2+<911::aid-cncr2820420713>3.0.co;2-g. [DOI] [PubMed] [Google Scholar]
  5. Bramwell V. H., Crowther D., Gallagher J., Stoddart R. W. Studies of lectin binding to normal and neoplastic lymphoid tissues. I. Normal nodes and Hodgkin's disease. Br J Cancer. 1982 Oct;46(4):568–581. doi: 10.1038/bjc.1982.241. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dorfman R. F. Letter: Classification of non-Hodgkin's lymphomas. Lancet. 1974 Oct 19;2(7886):961–962. doi: 10.1016/s0140-6736(74)91177-5. [DOI] [PubMed] [Google Scholar]
  7. Habeshaw J. A., Catley P. F., Stansfeld A. G., Brearley R. L. Surface phenotyping, histology and the nature of non-Hodgkin lymphoma in 157 patients. Br J Cancer. 1979 Jul;40(1):11–34. doi: 10.1038/bjc.1979.137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Levy R., Warnke R., Dorfman R. F., Haimovich J. The monoclonality of human B-cell lymphomas. J Exp Med. 1977 Apr 1;145(4):1014–1028. doi: 10.1084/jem.145.4.1014. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lukes R. J., Collins R. D. Lukes-Collins classification and its significance. Cancer Treat Rep. 1977 Sep;61(6):971–979. [PubMed] [Google Scholar]
  10. Lukes R. J., Taylor C. R., Parker J. W., Lincoln T. L., Pattengale P. K., Tindle B. H. A morphologic and immunologic surface marker study of 299 cases of non-Hodgkin lymphomas and related leukemias. Am J Pathol. 1978 Feb;90(2):461–486. [PMC free article] [PubMed] [Google Scholar]
  11. Mann R. B., Jaffe E. S., Berard C. W. Malignant lymphomas--a conceptual understanding of morphologic diversity. A review. Am J Pathol. 1979 Jan;94(1):105–192. [PMC free article] [PubMed] [Google Scholar]
  12. Nathwani B. N., Kim H., Rappaport H. Malignant lymphoma, lymphoblastic. Cancer. 1976 Aug;38(2):964–983. doi: 10.1002/1097-0142(197608)38:2<964::aid-cncr2820380248>3.0.co;2-v. [DOI] [PubMed] [Google Scholar]
  13. Risdall R., Hoppe R. T., Warnke R. Non-Hodgkin's lymphoma: a study of the evolution of the disease based upon 92 autopsied cases. Cancer. 1979 Aug;44(2):529–542. doi: 10.1002/1097-0142(197908)44:2<529::aid-cncr2820440222>3.0.co;2-y. [DOI] [PubMed] [Google Scholar]
  14. Rose M. L., Birbeck M. S., Wallis V. J., Forrester J. A., Davies A. J. Peanut lectin binding properties of germinal centres of mouse lymphoid tissue. Nature. 1980 Mar 27;284(5754):364–366. doi: 10.1038/284364a0. [DOI] [PubMed] [Google Scholar]
  15. Rose M. L., Habeshaw J. A., Kennedy R., Sloane J., Wiltshaw E., Davies A. J. Binding of peanut lectin to germinal-centre cells: a marker for B-cell subsets of follicular lymphoma? Br J Cancer. 1981 Jul;44(1):68–74. doi: 10.1038/bjc.1981.149. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Seligmann M., Brouet J. C., Preud'homme J. L. Immunologic classification of non-Hodgkin's lymphomas: current status. Cancer Treat Rep. 1977 Sep;61(6):1179–1183. [PubMed] [Google Scholar]
  17. Stein R. S., Cousar J., Flexner J. M., Graber S. E., McKee L. C., Krantz S., Collins R. C. Malignant lymphomas of follicular center cell origin in man. III. Prognostic features. Cancer. 1979 Dec;44(6):2236–2243. doi: 10.1002/1097-0142(197912)44:6<2236::aid-cncr2820440635>3.0.co;2-e. [DOI] [PubMed] [Google Scholar]
  18. Vernon S., Voet R. L., Naeim F., Waisman J. Nodular lymphoma with intracellular immunoglobulin. Cancer. 1979 Oct;44(4):1273–1279. doi: 10.1002/1097-0142(197910)44:4<1273::aid-cncr2820440416>3.0.co;2-x. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES