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. 1983 Mar;47(3):373–381. doi: 10.1038/bjc.1983.56

Characterization of human colon carcinoma cell lines isolated from a single primary tumour.

M G Brattain, M E Marks, J McCombs, W Finely, D E Brattain
PMCID: PMC2011309  PMID: 6830688

Abstract

The initiation of a cultured human colon carcinoma line on a feeder layer of confluent fibroblasts is described. Attempts to initiate cultures without fibroblast feeder layers were not successful. Two sub-lines (designated HCT C and HCT C Col) were isolated and weaned from cells growing on the surface of the feeder layer. The sub-lines had different morphologies, secreted different levels of carcinoembryonic antigen (CEA) into the medium of confluent cultures and had slightly different karyotypes. Both sub-lines grew in semi-solid medium and formed xenografts when injected s.c. into athymic nude mice. Analysis of radioiodinated cell membrane components indicated small, but significant differences between the sub-lines.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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