Skip to main content
NCBI home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1983 Jun;47(6):833–840. doi: 10.1038/bjc.1983.138

Clonal variation in the arrest, survival and growth of RIF-1 mouse sarcoma cells in the lungs of C3H mice.

J G Reeve, P R Twentyman
PMCID: PMC2011358  PMID: 6860549

Abstract

The relationship between the long term retention of 125IUdR-labelled tumour cells in the lungs and the formation of pulmonary lesions, has been examined for six in vitro isolated RIF-1 clones. Following i.v. injection, the initial number of cells trapped in the lungs was close to 100% in all cases. However, the rates at which individual clones were subsequently cleared from the lungs and the fraction of persistently retained cells varied considerably. Whilst clones also differed markedly in their lung colony formation efficiency (L.C.F.E.) there was no clear correlation between the long term retention of tumour cells in the lungs and subsequent metastasis formation, even when the retention of one clone was artificially increased in the lungs by admixture with microspheres. The fate after injection of clone 16 which is retained well in the lungs but which is of low L.C.F.E. has been compared with that of clone 19 which is retained poorly in the lung but which is of high L.C.F.E., using in vitro clonogenic capacity as a measure of cell viability in the lungs. Our findings show that clone 16 cells arrested in the lungs are in a viable, albeit "dormant", state some 26 days post i.v. injection. In contrast, arrested clone 19 cells proliferate rapidly in the lungs. These data may indicate varying significance of tumour cell and host properties in the metastatic success or failure of individual RIF-1 clones.

Full text

PDF
833

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brown J. M., Parker E. T. Host treatments affecting artificial pulmonary metastases: interpretation of loss of radioactively labelled cells from lungs. Br J Cancer. 1979 Nov;40(5):677–688. doi: 10.1038/bjc.1979.248. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chambers A. F., Hill R. P., Ling V. Tumor heterogeneity and stability of the metastatic phenotype of mouse KHT sarcoma cells. Cancer Res. 1981 Apr;41(4):1368–1372. [PubMed] [Google Scholar]
  3. FISHER B., FISHER E. R. Experimental evidence in support of the dormant tumor cell. Science. 1959 Oct 9;130(3380):918–919. doi: 10.1126/science.130.3380.918. [DOI] [PubMed] [Google Scholar]
  4. Fidler I. J., Gersten D. M., Hart I. R. The biology of cancer invasion and metastasis. Adv Cancer Res. 1978;28:149–250. doi: 10.1016/s0065-230x(08)60648-x. [DOI] [PubMed] [Google Scholar]
  5. Fidler I. J., Gersten D. M., Riggs C. W. Relationship of host immune status to tumor cell arrest, distribution, and survival in experimental metastasis. Cancer. 1977 Jul;40(1):46–55. doi: 10.1002/1097-0142(197707)40:1<46::aid-cncr2820400110>3.0.co;2-t. [DOI] [PubMed] [Google Scholar]
  6. Fidler I. J., Nicolson G. L. Organ selectivity for implantation survival and growth of B16 melanoma variant tumor lines. J Natl Cancer Inst. 1976 Nov;57(5):1199–1202. doi: 10.1093/jnci/57.5.1199. [DOI] [PubMed] [Google Scholar]
  7. Hart I. R., Fidler I. J. Role of organ selectivity in the determination of metastatic patterns of B16 melanoma. Cancer Res. 1980 Jul;40(7):2281–2287. [PubMed] [Google Scholar]
  8. Hart I. R., Talmadge J. E., Fidler I. J. Metastatic behavior of a murine reticulum cell sarcoma exhibiting organ-specific growth. Cancer Res. 1981 Apr;41(4):1281–1287. [PubMed] [Google Scholar]
  9. Liotta L. A., DeLisi C. Method for quantitating tumor cell removal and tumor cell-invasive capacity in experimental metastases. Cancer Res. 1977 Nov;37(11):4003–4008. [PubMed] [Google Scholar]
  10. Nicolson G. L., Brunson K. W., Fidler I. J. Specificity of arrest, survival, and growth of selected metastatic variant cell lines. Cancer Res. 1978 Nov;38(11 Pt 2):4105–4111. [PubMed] [Google Scholar]
  11. Poste G., Fidler I. J. The pathogenesis of cancer metastasis. Nature. 1980 Jan 10;283(5743):139–146. doi: 10.1038/283139a0. [DOI] [PubMed] [Google Scholar]
  12. Poste G., Nicolson G. L. Arrest and metastasis of blood-borne tumor cells are modified by fusion of plasma membrane vesicles from highly metastatic cells. Proc Natl Acad Sci U S A. 1980 Jan;77(1):399–403. doi: 10.1073/pnas.77.1.399. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Proctor J. W. Rat sarcoma model supports both "soil seed" and "mechanical" theories of metastatic spread. Br J Cancer. 1976 Dec;34(6):651–654. doi: 10.1038/bjc.1976.227. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Reeve J. G., Twentyman P. R. Ploidy distribution of tumour cells derived from induced and spontaneously arising metastases of a murine radiation-induced sarcoma, RIF-1. Eur J Cancer Clin Oncol. 1982 Oct;18(10):1001–1006. doi: 10.1016/0277-5379(82)90249-8. [DOI] [PubMed] [Google Scholar]
  15. Rieber M., Rieber M. S. Metastatic potential correlates with cell-surface protein alterations in B16 melanoma variants. Nature. 1981 Sep 3;293(5827):74–76. doi: 10.1038/293074a0. [DOI] [PubMed] [Google Scholar]
  16. Shearman P. J., Gallatin W. M., Longenecker B. M. Detection of a cell-surface antigen correlated with organ-specific metastasis. Nature. 1980 Jul 17;286(5770):267–269. doi: 10.1038/286267a0. [DOI] [PubMed] [Google Scholar]
  17. Suzuki N., Williams M., Hunter N. M., Withers H. R. Malignant properties and DNA content of daughter clones from a mouse fibrosarcoma: differentiation between malignant properties. Br J Cancer. 1980 Nov;42(5):765–771. doi: 10.1038/bjc.1980.310. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Twentyman P. R., Brown J. M., Gray J. W., Franko A. J., Scoles M. A., Kallman R. F. A new mouse tumor model system (RIF-1) for comparison of end-point studies. J Natl Cancer Inst. 1980 Mar;64(3):595–604. [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES