Skip to main content
NCBI home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1983 May;47(5):681–686. doi: 10.1038/bjc.1983.106

Characterisation of Epstein-Barr virus-specific memory T cells from the peripheral blood of seropositive individuals.

D H Crawford, V Iliescu, A J Edwards, P C Beverley
PMCID: PMC2011378  PMID: 6303377

Abstract

We have investigated the regression phenomenon which occurs when EBV-infected peripheral blood mononuclear cells from seropositive individuals are cultured for one month at high cell concentration and have confirmed that regression is mediated by E+ lymphocytes. When helper/inducer (Leu 3a+) and suppressor/cytotoxic (Leu 2a+) cells are separated by fluorescence-activated cell sorting from fresh peripheral blood and co-cultured with EBV-infected autologous E- mononuclear cells, regression only regularly occurs in cultures receiving suppressor/cytotoxic lymphocytes. Titration experiments show that suppressor/cytotoxic lymphocytes are more active in the regression assay that unfractionated E+ cells. When Ia+ E+ and Ia- E+ cells are separated one week after initiation of co-cultures of E+ cells and EBV-infected E- cells, both Ia+ E+ and Ia- E+ cells are active in the regression assay although regression occurs earlier in cultures receiving Ia+ E+ cells. Experiments in which NK cells are isolated using the monoclonal antibodies H25 and H366 show that NK cells do not influence the regression phenomenon in normal individuals.

Full text

PDF
681

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bird A. G., McLachlan S. M., Britton S. Cyclosporin A promotes spontaneous outgrowth in vitro of Epstein-Barr virus-induced B-cell lines. Nature. 1981 Jan 22;289(5795):300–301. doi: 10.1038/289300a0. [DOI] [PubMed] [Google Scholar]
  2. Callard R. E., Smith C. M., Beverley P. C. Phenotype of human T helper and suppressor cells in an in vitro specific antibody response. Eur J Immunol. 1982 Mar;12(3):232–236. doi: 10.1002/eji.1830120312. [DOI] [PubMed] [Google Scholar]
  3. Epstein M. A. Epstein-Barr virus--discovery, properties and relationship to nasopharyngeal carcinoma. IARC Sci Publ. 1978;(20):333–345. [PubMed] [Google Scholar]
  4. Evans A. S., Niederman J. C., McCollum R. W. Seroepidemiologic studies of infectious mononucleosis with EB virus. N Engl J Med. 1968 Nov 21;279(21):1121–1127. doi: 10.1056/NEJM196811212792101. [DOI] [PubMed] [Google Scholar]
  5. Evans R. L., Faldetta T. J., Humphreys R. E., Pratt D. M., Yunis E. J., Schlossman S. F. Peripheral human T cells sensitized in mixed leukocyte culture synthesize and express Ia-like antigens. J Exp Med. 1978 Nov 1;148(5):1440–1445. doi: 10.1084/jem.148.5.1440. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Golden H. D., Chang R. S., Prescott W., Simpson E., Cooper T. Y. Leukocyte-transforming agent: prolonged excretion by patients with mononucleosis and excretion by normal individuals. J Infect Dis. 1973 Apr;127(4):471–473. doi: 10.1093/infdis/127.4.471. [DOI] [PubMed] [Google Scholar]
  7. Henle G., Henle W., Diehl V. Relation of Burkitt's tumor-associated herpes-ytpe virus to infectious mononucleosis. Proc Natl Acad Sci U S A. 1968 Jan;59(1):94–101. doi: 10.1073/pnas.59.1.94. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hewetson J. F., Rocchi G., Henle W., Henle G. Neutralizing antibodies to Epstein-Barr virus in healthy populations and patients with infectious mononucleosis. J Infect Dis. 1973 Sep;128(3):283–289. doi: 10.1093/infdis/128.3.283. [DOI] [PubMed] [Google Scholar]
  9. Kaplan M. E., Clark C. An improved rosetting assay for detection of human T lymphocytes. J Immunol Methods. 1974 Jul;5(2):131–135. doi: 10.1016/0022-1759(74)90003-9. [DOI] [PubMed] [Google Scholar]
  10. Miller G., Shope T., Lisco H., Stitt D., Lipman M. Epstein-Barr virus: transformation, cytopathic changes, and viral antigens in squirrel monkey and marmoset leukocytes. Proc Natl Acad Sci U S A. 1972 Feb;69(2):383–387. doi: 10.1073/pnas.69.2.383. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Misko I. S., Kane R. G., Pope J. H. Generation in vitro of HLA-restricted EB virus-specific cytotoxic human T cells by autologous lymphoblastoid cell lines: the role of previous EB virus infection and foetal calf serum. Int J Cancer. 1982 Jan 15;29(1):41–48. doi: 10.1002/ijc.2910290108. [DOI] [PubMed] [Google Scholar]
  12. Moss D. J., Rickinson A. B., Pope J. H. Long-term T-cell-mediated immunity to Epstein-Barr virus in man. I. Complete regression of virus-induced transformation in cultures of seropositive donor leukocytes. Int J Cancer. 1978 Dec;22(6):662–668. doi: 10.1002/ijc.2910220604. [DOI] [PubMed] [Google Scholar]
  13. Moss D. J., Wallace L. E., Rickinson A. B., Epstein M. A. Cytotoxic T cell recognition of Epstein-Barr virus-infected B cells. I. Specificity and HLA restriction of effector cells reactivated in vitro. Eur J Immunol. 1981 Sep;11(9):686–693. doi: 10.1002/eji.1830110904. [DOI] [PubMed] [Google Scholar]
  14. Nilsson K., Klein G., Henle W., Henle G. The establishment of lymphoblastoid lines from adult and fetal human lymphoid tissue and its dependence on EBV. Int J Cancer. 1971 Nov 15;8(3):443–450. doi: 10.1002/ijc.2910080312. [DOI] [PubMed] [Google Scholar]
  15. Palacios R., Möller G. Cyclosporin A blocks receptors for HLA-DR antigens on T cells. Nature. 1981 Apr 30;290(5809):792–794. doi: 10.1038/290792a0. [DOI] [PubMed] [Google Scholar]
  16. Pereira R. S., Doré C. J., Gear A. J. Rheumatoid lymphocyte defect revealed by cyclosporin A. Lancet. 1982 Sep 25;2(8300):721–722. doi: 10.1016/s0140-6736(82)90746-2. [DOI] [PubMed] [Google Scholar]
  17. Rickinson A. B., Moss D. J., Pope J. H. Long-term C-cell-mediated immunity to Epstein-Barr virus in man. II. Components necessary for regression in virus-infected leukocyte cultures. Int J Cancer. 1979 May 15;23(5):610–617. doi: 10.1002/ijc.2910230505. [DOI] [PubMed] [Google Scholar]
  18. Rickinson A. B., Wallace L. E., Epstein M. A. HLA-restricted T-cell recognition of Epstein-Barr virus-infected B cells. Nature. 1980 Feb 28;283(5750):865–867. doi: 10.1038/283865a0. [DOI] [PubMed] [Google Scholar]
  19. Thomas Y., Sosman J., Rogozinski L., Irigoyen O., Kung P. C., Goldstein G., Chess L. Functional analysis of human T cell subsets defined by monoclonal antibodies. III. Regulation of helper factor production by T cell subsets. J Immunol. 1981 May;126(5):1948–1951. [PubMed] [Google Scholar]
  20. Thorley-Lawson D. A. The transformation of adult but not newborn human lymphocytes by Epstein Barr virus and phytohemagglutinin is inhibited by interferon: the early suppression by T cells of Epstein Barr infection is mediated by interferon. J Immunol. 1981 Mar;126(3):829–833. [PubMed] [Google Scholar]
  21. Tsoukas C. D., Fox R. I., Slovin S. F., Carson D. A., Pellegrino M., Fong S., Pasquali J. L., Ferrone S., Kung P., Vaughan J. H. T lymphocyte-mediated cytotoxicity against autologous EBV-genome-bearing B cells. J Immunol. 1981 May;126(5):1742–1746. [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES