Skip to main content
NCBI home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1983 May;47(5):687–695. doi: 10.1038/bjc.1983.107

Human natural killing against ovarian carcinoma.

H Shau, H S Koren, J R Dawson
PMCID: PMC2011385  PMID: 6573908

Abstract

Natural killing (NK) by lymphocytes from normal individuals against primary and established ovarian carcinoma cell lines was tested in short-term chromium release assays. Two established cell lines and 5/6 primary cell lines tested showed significant susceptibility to NK. Primary cell lines are, in general, less sensitive to NK than long-term cultured target cells. A common NK recognition determinant on ovarian carcinoma cells and on the erythroleukaemic K562 cells was demonstrated by cold target inhibition assays. The recognition structure was also present on an ovarian cell line resistant to NK but not on the insensitive leukaemic cell line, SB. The activity against ovarian carcinoma cells was associated with the presence of large granular lymphocytes (LGL) previously shown to be the major effector cells against K562 targets. In fractions obtained by Percoll gradient centrifugation of lymphocytes, only fractions with a high content of LGL demonstrated good NK activity. LGL mediated NK against both non-adherent K562 and the adherent ovarian carcinoma target cells independent of monocytes.

Full text

PDF
687

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Armstrong J. A. Semi-micro, dye-binding assay for rabbit interferon. Appl Microbiol. 1971 Apr;21(4):723–725. doi: 10.1128/am.21.4.723-725.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Callewaert D. M., Kaplan J., Johnson D. F., Peterson W. D., Jr Spontaneous cytotoxicity of cultured human cell lines mediated by normal peripheral blood lymphocytes. II. Specificity for target antigens. Cell Immunol. 1979 Jan;42(1):103–112. doi: 10.1016/0008-8749(79)90225-9. [DOI] [PubMed] [Google Scholar]
  3. KAPLOW L. S. SIMPLIFIED MYELOPEROXIDASE STAIN USING BENZIDINE DIHYDROCHLORIDE. Blood. 1965 Aug;26:215–219. [PubMed] [Google Scholar]
  4. Kadish A. S., Doyle A. T., Steinhauer E. H., Ghossein N. A. Natural cytotoxicity and interferon production in human cancer: deficient natural killer activity and normal interferon production in patients with advanced disease. J Immunol. 1981 Nov;127(5):1817–1822. [PubMed] [Google Scholar]
  5. Koren H. S., Williams M. S. Natural killing and antibody-dependent cellular cytotoxicity are mediated by different mechanisms and by different cells. J Immunol. 1978 Nov;121(5):1956–1960. [PubMed] [Google Scholar]
  6. Mantovani A., Allavena P., Sessa C., Bolis G., Mangioni C. Natural killer activity of lymphoid cells isolated from human ascitic ovarian tumors. Int J Cancer. 1980 May 15;25(5):573–582. doi: 10.1002/ijc.2910250505. [DOI] [PubMed] [Google Scholar]
  7. Ortaldo J. R., Oldham R. K., Cannon G. C., Herberman R. B. Specificity of natural cytotoxic reactivity of normal human lymphocytes against a myeloid leukemia cell line. J Natl Cancer Inst. 1977 Jul;59(1):77–82. doi: 10.1093/jnci/59.1.77. [DOI] [PubMed] [Google Scholar]
  8. Roder J. C., Rosén A., Fenyö E. M., Troy F. A. Target-effector interaction in the natural killer cell system: isolation of target structures. Proc Natl Acad Sci U S A. 1979 Mar;76(3):1405–1409. doi: 10.1073/pnas.76.3.1405. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Shau H., Dawson J. R. Regulation of human natural killing by levamisole. Cancer Immunol Immunother. 1982;13(1):24–29. doi: 10.1007/BF00200196. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Timonen T., Ortaldo J. R., Herberman R. B. Characteristics of human large granular lymphocytes and relationship to natural killer and K cells. J Exp Med. 1981 Mar 1;153(3):569–582. doi: 10.1084/jem.153.3.569. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Timonen T., Saksela E. Isolation of human NK cells by density gradient centrifugation. J Immunol Methods. 1980;36(3-4):285–291. doi: 10.1016/0022-1759(80)90133-7. [DOI] [PubMed] [Google Scholar]
  12. Timonen T., Saksela E., Ranki A., Häyry P. Fractionation, morphological and functional characterization of effector cells responsible for human natural killer activity against cell-line targets. Cell Immunol. 1979 Nov;48(1):133–148. doi: 10.1016/0008-8749(79)90106-0. [DOI] [PubMed] [Google Scholar]
  13. Uchida A., Kolb R., Micksche M. Generation of suppressor cells for natural killer activity in cancer patients after surgery. J Natl Cancer Inst. 1982 May;68(5):735–741. [PubMed] [Google Scholar]
  14. Vose B. M., Moore M. Natural cytotoxicity in humans: susceptibility of freshly isolatd tumor cells to lysis. J Natl Cancer Inst. 1980 Aug;65(2):257–263. [PubMed] [Google Scholar]
  15. Vánky F. T., Argov S. A., Einhorn S. A., Klein E. Role of alloantigens in natural killing. Allogeneic but not autologous tumor biopsy cells are sensitive for interferon-induced cytotoxicity of human blood lymphcoytes. J Exp Med. 1980 May 1;151(5):1151–1165. doi: 10.1084/jem.151.5.1151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. de Landazuri M. O., López-Botet M., Timonen T., Ortaldo J. R., Herberman R. B. Human large granular lymphocytes: spontaneous and interferon-boosted NK activity against adherent and nonadherent tumor cell lines. J Immunol. 1981 Oct;127(4):1380–1383. [PubMed] [Google Scholar]
  17. de Vries J. E., Mendelsohn J., Bont W. S. The role of target cells, monocytes, and Fc receptor-bearing lymphocytes in human spontaneous cell-mediated cytotoxicity and antibody-dependent cellular cytotoxicity. J Immunol. 1980 Jul;125(1):396–405. [PubMed] [Google Scholar]
  18. de Vries J. E., Meyering M., van Dongen A., Rümke P. The influence of different isolation procedures and the use of target cells from melanoma cell lines and short-term cultures on the non-specific cytotoxic effects of lymphocytes from healthy donors. Int J Cancer. 1975 Mar 15;15(3):391–400. doi: 10.1002/ijc.2910150305. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES