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British Journal of Cancer logoLink to British Journal of Cancer
. 1983 Dec;48(6):797–801. doi: 10.1038/bjc.1983.269

Sister chromatid exchange in lymphocytes from renal transplant recipients with and without cancer.

G E Kelly, A G Sheil
PMCID: PMC2011574  PMID: 6360193

Abstract

The frequency of sister chromatid exchange (SCE) was measured in peripheral blood lymphocytes from control, healthy subjects and immunosuppressed recipients of cadaveric donor kidneys with and without skin cancer. The mean SCE frequency in 43 control subjects was 9.2 per cell (range 5.4-12.3). In 30 transplant recipients with no history or evidence of cancer the mean SCE rate was 10.3 per cell (range 5.8-24.5); four (13%) of these patients had a mean SCE frequency outside the control range. In 7 transplant recipients with skin cancer, the mean SCE frequency was 14.3 per cell (range 9.1-19.9). This was significantly (P less than 0.01) higher than the mean value of control subjects. The mean SCE frequencies in 3 of these 7 patients fell within the control range and in 4 of these patients was above the control range. These results suggest that some immunosuppressed kidney transplant recipients are liable to chromosomal damage.

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Selected References

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  1. Abe S., Sasaki M. Chromosome aberrations and sister chromatid exchanges in Chinese hamster cells exposed to various chemicals. J Natl Cancer Inst. 1977 Jun;58(6):1635–1641. doi: 10.1093/jnci/58.6.1635. [DOI] [PubMed] [Google Scholar]
  2. Clark J. M. The mutagenicity of azathioprine in mice, Drosophila melanogaster and Neurospora crassa. Mutat Res. 1975 Apr;28(1):87–99. doi: 10.1016/0027-5107(75)90318-8. [DOI] [PubMed] [Google Scholar]
  3. Crossen P. E., Drets M. E., Arrighi F. E., Johnston D. A. Aanlysis of the frequency and distribution of sister chromatid exchanges in cultured human lymphocytes. Hum Genet. 1977 Mar 14;35(3):345–352. doi: 10.1007/BF00446625. [DOI] [PubMed] [Google Scholar]
  4. Eberle P., Hunstein W., Perings E. Chromosomes in patients treated with Imuran. Humangenetik. 1968;6(1):69–73. doi: 10.1007/BF00287156. [DOI] [PubMed] [Google Scholar]
  5. GITHENS J. H., ROSENKRANTZ J. G., TUNNOCK S. M. TERATOGENIC EFFECTS OF AZATHIOPRINE (IMURAN). J Pediatr. 1965 May;66:959–961. doi: 10.1016/s0022-3476(65)80071-3. [DOI] [PubMed] [Google Scholar]
  6. Galloway S. M., Evans H. J. Sister chromatid exchange in human chromosomes from normal individuals and patients with ataxia telangiectasia. Cytogenet Cell Genet. 1975;15(1):17–29. doi: 10.1159/000130495. [DOI] [PubMed] [Google Scholar]
  7. Goto K., Akematsu T., Shimazu H., Sugiyama T. Simple differential Giemsa staining of sister chromatids after treatment with photosensitive dyes and exposure to light and the mechanism of staining. Chromosoma. 1975 Dec 10;53(3):223–230. doi: 10.1007/BF00329173. [DOI] [PubMed] [Google Scholar]
  8. Jensen M. K. Chromosome studies in patients treated with azathioprine and amethopterin. Acta Med Scand. 1967 Oct;182(4):445–455. doi: 10.1111/j.0954-6820.1967.tb10869.x. [DOI] [PubMed] [Google Scholar]
  9. Jensen M. K. Effect of azathioprine on the chromosome complement of human bone marrow cells. Int J Cancer. 1970 Jan 15;5(1):147–151. doi: 10.1002/ijc.2910050119. [DOI] [PubMed] [Google Scholar]
  10. Jensen M. K., Hüttel M. S. Assessment of the effect of azathioprine on human bone marrow cells in vivo, combining chromosome studies and the micronucleus test. Dan Med Bull. 1976 Jun;23(3):152–154. [PubMed] [Google Scholar]
  11. Jensen M. K., Soborg M. Chromosome aberrations in human cells following treatment with imuran. Preliminary report. Acta Med Scand. 1966 Feb;179(2):249–250. doi: 10.1111/j.0954-6820.1966.tb05454.x. [DOI] [PubMed] [Google Scholar]
  12. Kingston A., Harnden D. G., Woodruff M. F., Nolan B., Robson J. S. Studies on the lymphocytes of patients with renal homografts. Transplantation. 1971 Oct;12(4):305–309. doi: 10.1097/00007890-197110000-00012. [DOI] [PubMed] [Google Scholar]
  13. Latt S. A. Sister chromatid exchanges, indices of human chromosome damage and repair: detection by fluorescence and induction by mitomycin C. Proc Natl Acad Sci U S A. 1974 Aug;71(8):3162–3166. doi: 10.1073/pnas.71.8.3162. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Leb D. E., Weisskopf B., Kanovitz B. S. Chromosome aberrations in the child of a kidney transplant recipient. Arch Intern Med. 1971 Sep;128(3):441–444. [PubMed] [Google Scholar]
  15. Morgan W. F., Crossen P. E. The incidence of sister chromatid exchanges in cultured human lymphocytes. Mutat Res. 1977 Feb;42(2):305–311. doi: 10.1016/s0027-5107(77)80032-8. [DOI] [PubMed] [Google Scholar]
  16. Nasjleti C. E., Spencer H. H. Chromosome damage and polyploidization induced in human peripheral leukocytes in vivo and in vitro with nitrogen mustard, 6-mercaptopurine, and A-649. Cancer Res. 1966 Dec;26(12):2437–2443. [PubMed] [Google Scholar]
  17. Nevstad N. P. Sister chromatid exchanges and chromosomal aberrations induced in human lymphocytes by the cytostatic drug adriamycin in vivo and in vitro. Mutat Res. 1978 May;57(2):253–258. doi: 10.1016/0027-5107(78)90275-0. [DOI] [PubMed] [Google Scholar]
  18. Ockey C. H. Differences between "spontaneous" and induced sister-chromatid exchanges with fixation time and their chromosome localization. Cytogenet Cell Genet. 1980;26(2-4):223–235. doi: 10.1159/000131443. [DOI] [PubMed] [Google Scholar]
  19. Penn I. Tumor incidence in human allograft recipients. Transplant Proc. 1979 Mar;11(1):1047–1051. [PubMed] [Google Scholar]
  20. Perry P., Evans H. J. Cytological detection of mutagen-carcinogen exposure by sister chromatid exchange. Nature. 1975 Nov 13;258(5531):121–125. doi: 10.1038/258121a0. [DOI] [PubMed] [Google Scholar]
  21. Perry P., Wolff S. New Giemsa method for the differential staining of sister chromatids. Nature. 1974 Sep 13;251(5471):156–158. doi: 10.1038/251156a0. [DOI] [PubMed] [Google Scholar]
  22. Raposa T. Sister chromatid exchange studies for monitoring DNA damage and repair capacity after cytostatics in vitro and in lymphocytes of leukaemic patients under cytostatic therapy. Mutat Res. 1978 May;57(2):241–251. doi: 10.1016/0027-5107(78)90274-9. [DOI] [PubMed] [Google Scholar]
  23. Sheil A. G., Mahony J. F., Horvath J. S., Johnson J. R., Tiller D. J., Stewart J. H., May J. Cancer following successful cadaveric donor renal transplantation. Transplant Proc. 1981 Mar;13(1 Pt 2):733–735. [PubMed] [Google Scholar]
  24. Sheil A. G., Stewart J. H., Johnson J. R., May J., Storey B. G., Rogers J. H., Charlesworth J. A., Hurley B. P., Tiller D., Mahony J. F. Cadaveric donor renal transplantation. Med J Aust. 1972 Jan 29;1(5):205–209. doi: 10.5694/j.1326-5377.1972.tb46764.x. [DOI] [PubMed] [Google Scholar]
  25. Solomon E., Bobrow M. Sister chromatid exchanges--a sensitive assay of agents damaging human chromosomes. Mutat Res. 1975 Nov;30(2):273–278. [PubMed] [Google Scholar]
  26. Speck W. T., Rosenkranz H. S. Mutagenicity of azathioprine. Cancer Res. 1976 Jan;36(1):108–109. [PubMed] [Google Scholar]
  27. Stetka D. G., Minkler J., Carrano A. V. Induction of long-lived chromosome damage, as manifested by sister-chromatid exchange, in lymphocytes of animals exposed to mitomycin-C. Mutat Res. 1978 Sep;51(3):383–396. doi: 10.1016/0027-5107(78)90126-4. [DOI] [PubMed] [Google Scholar]
  28. THIERSCH J. B. Effect of 6-(1'-methyl-4'-nitro-5'-imidazolyl)-mercaptopurine and 2-amino-6-(1'-methyl-4'-nitro-5'-imidazolyl)-mercaptopurine on the rat litter in utero. J Reprod Fertil. 1962 Dec;4:297–302. doi: 10.1530/jrf.0.0040297. [DOI] [PubMed] [Google Scholar]
  29. Wyrobek A. J., Bruce W. R. Chemical induction of sperm abnormalities in mice. Proc Natl Acad Sci U S A. 1975 Nov;72(11):4425–4429. doi: 10.1073/pnas.72.11.4425. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. van Went G. F. Investigation into the mutagenic activity of azathioprine (Imuran) in different test systems. Mutat Res. 1979 Oct;68(2):153–162. doi: 10.1016/0165-1218(79)90143-5. [DOI] [PubMed] [Google Scholar]

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