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British Journal of Experimental Pathology logoLink to British Journal of Experimental Pathology
. 1987 Feb;68(1):71–80.

Antiserum-induced neutropenia in the rat: characterization of a rabbit anti-rat neutrophil serum.

H Sandler, H Högstorp, C Lundberg, B Gerdin
PMCID: PMC2012998  PMID: 3814503

Abstract

A heterologous rabbit anti-rat neutrophil serum (ANS) based on peptone-stimulated peritoneal exudate neutrophils (PMNLs) from Sprague Dawley rats was prepared. Leucoagglutination and indirect immunofluorescence assays revealed high titres of antibodies to rat PMNLs (1/2560), lower titre of antibodies to rat lymphocytes (1/160) and a very low titre against rat platelets (1/20). ANS given intravenously (i.v.) to rats in doses of up to 42 mg of protein/kg b.w. caused transient neutropenia, lasting about 10 min after administration, and thrombocytopenia, lasting about 5 min. Two minutes after an i.v. injection of 21 mg of ANS/kg b.w. there was profound uptake of 51Cr-labelled PMNLs in the lung, increased release of 51Cr to plasma, an increased amount of 51Cr in the spleen and consumption of greater than 98% of total complement (CH50). Two hours later there was high activity of 51Cr in the plasma, spleen and liver, while lung radioactivity had decreased to below baseline and CH50 had recovered to 55% of baseline. An intraperitoneal (i.p.) injection of ANS was followed by prolonged neutropenia with a maximum after 12 h. Simultaneously peripheral mononuclear cells slightly decreased. There was no change in the number of peripheral platelets in the blood or in the plasma concentration of fibrinogen, alpha 2-antiplasmin, plasminogen or plasminogen activators. Intraperitoneal administration of ANS did not affect CH50. It was concluded that the raised ANS had good specificity against rat PMNLs and was able to induce prolonged neutropenia after i.p. injection without affecting the complement of fibrinolytic system.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BIERMAN H. R., KELLY K. H., CORDES F. L. The sequestration and visceral circulation of leukocytes in man. Ann N Y Acad Sci. 1955 Mar 24;59(5):850–862. doi: 10.1111/j.1749-6632.1955.tb45987.x. [DOI] [PubMed] [Google Scholar]
  2. Flick M. R., Perel A., Staub N. C. Leukocytes are required for increased lung microvascular permeability after microembolization in sheep. Circ Res. 1981 Mar;48(3):344–351. doi: 10.1161/01.res.48.3.344. [DOI] [PubMed] [Google Scholar]
  3. Högstorp H., Jakobsson H., Carlin G. Studies on alpha 2-antiplasmin in the rat. Thromb Res. 1981 Feb 1;21(3):247–253. doi: 10.1016/0049-3848(81)90162-6. [DOI] [PubMed] [Google Scholar]
  4. Lawrence J. S., Craddock C. G., Jr, Campbell T. N. Antineutrophilic serum, its use in studies of white blood cell dynamics. J Lab Clin Med. 1967 Jan;69(1):88–101. [PubMed] [Google Scholar]
  5. Lundberg C., Arfors K. E. Polymorphonuclear leukocyte accumulation in inflammatory dermal sites as measured by 51Cr-labeled cells and myeloperoxidase. Inflammation. 1983 Sep;7(3):247–255. doi: 10.1007/BF00917262. [DOI] [PubMed] [Google Scholar]
  6. NILSSON I. M., OLOW B. Determination of fibrinogen and fibrinogenolytic activity. Thromb Diath Haemorrh. 1962 Nov 15;8:297–310. [PubMed] [Google Scholar]
  7. Nilsson U. R., Nilsson B. Simplified assays of hemolytic activity of the classical and alternative complement pathways. J Immunol Methods. 1984 Aug 3;72(1):49–59. doi: 10.1016/0022-1759(84)90432-0. [DOI] [PubMed] [Google Scholar]
  8. Shasby D. M., Fox R. B., Harada R. N., Repine J. E. Reduction of the edema of acute hyperoxic lung injury by granulocyte depletion. J Appl Physiol Respir Environ Exerc Physiol. 1982 May;52(5):1237–1244. doi: 10.1152/jappl.1982.52.5.1237. [DOI] [PubMed] [Google Scholar]
  9. Simpson D. M., Ross R. The neutrophilic leukocyte in wound repair a study with antineutrophil serum. J Clin Invest. 1972 Aug;51(8):2009–2023. doi: 10.1172/JCI107007. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Till G. O., Johnson K. J., Kunkel R., Ward P. A. Intravascular activation of complement and acute lung injury. Dependency on neutrophils and toxic oxygen metabolites. J Clin Invest. 1982 May;69(5):1126–1135. doi: 10.1172/JCI110548. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. WARD P. A., COCHRANE C. G. BOUND COMPLEMENT AND IMMUNOLOGIC INJURY OF BLOOD VESSELS. J Exp Med. 1965 Feb 1;121:215–234. doi: 10.1084/jem.121.2.215. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Wedmore C. V., Williams T. J. Control of vascular permeability by polymorphonuclear leukocytes in inflammation. Nature. 1981 Feb 19;289(5799):646–650. doi: 10.1038/289646a0. [DOI] [PubMed] [Google Scholar]

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