Skip to main content
NCBI home page
British Journal of Experimental Pathology logoLink to British Journal of Experimental Pathology
. 1987 Apr;68(2):167–177.

Epithelial cell proliferation in the rat urinary system induced by parenteral injection of lead salts.

B M Herbertson, A J King, J Allen
PMCID: PMC2013013  PMID: 3580279

Abstract

In Wistar rats a single intravascular injection of lead nitrate causes substantial cellular proliferation in the proximal tubules of the kidneys and in the epithelium of the renal pelvis, ureters and urinary bladder. The tritiated thymidine labelling index (LI) reaches a peak 18 to 24 h after injection and the mitotic index (MI) increases to a comparable extent 6 to 10 h later. The proliferation is most prominent in the bladder and proximal tubules and is virtually complete at 48 h. After a single intraperitoneal injection the proliferative changes are confined to the ureteric and bladder epithelium. In all affected tissues the proliferation develops without preceding necrosis and appears to be a hyperplastic phenomenon. After intravascular administration the volume of urine and its total protein content are significantly increased and certain proteins were detected which were not present in the urine of saline controls.

Full text

PDF
167

Images in this article

174Fig. 3
on p.174

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Choie D. D., Richter G. W. Cell proliferation in mouse kidney induced by lead. I. Synthesis of deoxyribonucleic acid. Lab Invest. 1974 May;30(5):647–651. [PubMed] [Google Scholar]
  2. Choie D. D., Richter G. W. Cell proliferation in rat kidney induced by lead acetate and effects of uninephrectomy on the proliferation. Am J Pathol. 1972 Feb;66(2):265–275. [PMC free article] [PubMed] [Google Scholar]
  3. FINLAYSON J. S., MORRIS H. P. MOLECULAR SIZE OF RAT URINARY PROTEIN. Proc Soc Exp Biol Med. 1965 Jul;119:663–666. doi: 10.3181/00379727-119-30265. [DOI] [PubMed] [Google Scholar]
  4. Herbertson B. M., King A. J., Allen J. Dna synthesis in the urinary-tract epithelium of the rat induced by laparotomy and by intraperitoneal injection of distilled water, physiological saline and lycopodium spores. Br J Exp Pathol. 1982 Dec;63(6):594–605. [PMC free article] [PubMed] [Google Scholar]
  5. Herbertson B. M., Steele P. R., Allen J. DNA synthesis in the urinary tract epithelium of the rat induced by ureteric ligation. Lab Invest. 1981 Sep;45(3):285–294. [PubMed] [Google Scholar]
  6. Hsueh W., Rostorfer H. H. Chemically induced renal hypertrophy in the rat. Lab Invest. 1973 Nov;29(5):547–555. [PubMed] [Google Scholar]
  7. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  8. Markwell M. A., Haas S. M., Bieber L. L., Tolbert N. E. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal Biochem. 1978 Jun 15;87(1):206–210. doi: 10.1016/0003-2697(78)90586-9. [DOI] [PubMed] [Google Scholar]
  9. Nikaido H., Yamada J., Kondo Y. Male urinary protein-1 (MUP-1) in the rat: Mup-1 assigned to linkage group II. J Hered. 1982 Mar-Apr;73(2):119–122. doi: 10.1093/oxfordjournals.jhered.a109593. [DOI] [PubMed] [Google Scholar]
  10. Roy A. K., Neuhaus O. W., Harmison C. R. Preparation and characterization of a sex-dependent rat urinary protein. Biochim Biophys Acta. 1966 Sep 26;127(1):72–81. doi: 10.1016/0304-4165(66)90477-6. [DOI] [PubMed] [Google Scholar]
  11. Threlfall G., Taylor D. M., Buck A. T. The effect of folic acid on growth and deoxyribonucleic acid synthesis in the rat kidney. Lab Invest. 1966 Sep;15(9):1477–1485. [PubMed] [Google Scholar]

Articles from British journal of experimental pathology are provided here courtesy of Wiley

RESOURCES