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British Journal of Experimental Pathology logoLink to British Journal of Experimental Pathology
. 1986 Feb;67(1):105–112.

Cytoskeletal changes as an early event in hydrogen peroxide-induced cell injury: a study in A549 cells.

G Raghu, L Striker, J Harlan, A Gown, G Striker
PMCID: PMC2013069  PMID: 2418861

Abstract

Hydrogen peroxide (H2O2) and other oxygen metabolites have been implicated in the pathogenesis of cell and tissue injury. The nature of the injury occurring in cells exposed to oxygen metabolites is unknown. A549 cells, derived from human lung carcinoma, were exposed to glucose-glucose oxidase or hydrogen peroxide in vitro. The distribution of actin and cytokeratin filaments, as well as 51chromium (51Cr) release and trypan blue dye exclusion were assessed. Both glucose-glucose oxidase and H2O2 resulted in changes which were time- and dose-dependent. Alterations in the cytoskeleton were detected by immunofluorescence microscopy at two hours, at which time the cells excluded trypan blue dye, while 51Cr release and trypan blue uptake first occurred at 8 h and required a five-fold greater concentration of glucose oxidase. The addition of catalase to glucose-glucose oxidase or H2O2, or inactivation of glucose oxidase by boiling, abrogated the injury. Therefore, one of the early targets of H2O2-induced cell injury may be the cytoskeleton.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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