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. 1994 Jan;60(1):328–332. doi: 10.1128/aem.60.1.328-332.1994

Changes in Bacterial Numbers and Leucine Assimilation during Estimations of Microbial Respiratory Rates in Seawater by the Precision Winkler Method

Lawrence R Pomeroy 1,*, Joan E Sheldon 1, Wade M Sheldon Jr 1
PMCID: PMC201307  PMID: 16349160

Abstract

During incubation of seawater in bottles, the decrease in dissolved oxygen is often nonlinear over time scales frequently used to measure respiration. Numbers of bacteria always increase, and rates of assimilation of dissolved leucine often increase exponentially. This suggests that sample handling disrupts the previously existing food web, leading to shifts of trophic state and unbalanced growth. Potential errors in measuring respiratory rate can be minimized by documenting these variables.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Carlucci A. F., Craven D. B., Henrichs S. M. Diel production and microheterotrophic utilization of dissolved free amino acids in waters off southern california. Appl Environ Microbiol. 1984 Jul;48(1):165–170. doi: 10.1128/aem.48.1.165-170.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Fuhrman J. A., Azam F. Bacterioplankton secondary production estimates for coastal waters of british columbia, antarctica, and california. Appl Environ Microbiol. 1980 Jun;39(6):1085–1095. doi: 10.1128/aem.39.6.1085-1095.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hobbie J. E., Daley R. J., Jasper S. Use of nuclepore filters for counting bacteria by fluorescence microscopy. Appl Environ Microbiol. 1977 May;33(5):1225–1228. doi: 10.1128/aem.33.5.1225-1228.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kirchman D., K'nees E., Hodson R. Leucine incorporation and its potential as a measure of protein synthesis by bacteria in natural aquatic systems. Appl Environ Microbiol. 1985 Mar;49(3):599–607. doi: 10.1128/aem.49.3.599-607.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]

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