Skip to main content
NCBI home page
British Journal of Experimental Pathology logoLink to British Journal of Experimental Pathology
. 1988 Jun;69(3):423–432.

Immunopathological study of spleen during Japanese encephalitis virus infection in mice.

A Mathur 1, M Bharadwaj 1, R Kulshreshtha 1, S Rawat 1, A Jain 1, U C Chaturvedi 1
PMCID: PMC2013103  PMID: 2839221

Abstract

Following intraperitoneal inoculation, Japanese encephalitis virus replicated in peritoneal macrophages, appeared on day 3 in the splenic macrophages of the perifollicular region and later in cells of the periarteriolar lymphoid sheath (PALS) as shown by indirect immunofluorescence. Productive JEV infection was observed both in macrophages and T-cells. Morphological study of spleen during JEV infection revealed proliferative changes, with increased number of macrophages from day 3 p.i. in the perifollicular region followed by accumulation of polymorphonuclear leucocytes which reached a maximum on day 9 p.i. The T dependent areas were considerably enlarged by day 9 and gradually reduced in size by week 3. At later periods germinal centres appeared in the T independent area and were prominent by day 15. The cells containing virus antigen disappeared with the appearance of germinal centres, thus indicating the role of the latter also in virus clearance.

Full text

PDF
423

Images in this article

425Fig. 1
on p.425
426Fig. 2
on p.426
427Fig. 3
on p.427

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CLARKE D. H., CASALS J. Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses. Am J Trop Med Hyg. 1958 Sep;7(5):561–573. doi: 10.4269/ajtmh.1958.7.561. [DOI] [PubMed] [Google Scholar]
  2. Chaturvedi U. C., Nagar R., Mathur A. Effect of dengue virus infection on Fc-receptor functions of mouse macrophages. J Gen Virol. 1983 Nov;64(Pt 11):2399–2407. doi: 10.1099/0022-1317-64-11-2399. [DOI] [PubMed] [Google Scholar]
  3. Cline M. J., Lehrer R. I. Phagocytosis by human monocytes. Blood. 1968 Sep;32(3):423–435. [PubMed] [Google Scholar]
  4. Gallo R. C., Salahuddin S. Z., Popovic M., Shearer G. M., Kaplan M., Haynes B. F., Palker T. J., Redfield R., Oleske J., Safai B. Frequent detection and isolation of cytopathic retroviruses (HTLV-III) from patients with AIDS and at risk for AIDS. Science. 1984 May 4;224(4648):500–503. doi: 10.1126/science.6200936. [DOI] [PubMed] [Google Scholar]
  5. Golub E. S. Brain-associated theta antigen: reactivity of rabbit anti-mouse brain with mouse lymphoid cells. Cell Immunol. 1971 Aug;2(4):353–361. doi: 10.1016/0008-8749(71)90070-0. [DOI] [PubMed] [Google Scholar]
  6. Howard R. J., Craig C. P., Trevino G. S., Dougherty S. F., Mergenhagen S. E. Enhanced humoral immunity in mice infected with attenuated Venezuelan equine encephalitis virus. J Immunol. 1969 Oct;103(4):699–707. [PubMed] [Google Scholar]
  7. Hyypiä T., Korkiamäki P., Vainionpä R. Replication of measles virus in human lymphocytes. J Exp Med. 1985 Jun 1;161(6):1261–1271. doi: 10.1084/jem.161.6.1261. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Isakov N., Feldman M., Segal S. Acute infection of mice with lactic dehydrogenase virus (LDV) impairs the antigen-presenting capacity of their macrophages. Cell Immunol. 1982 Jan 15;66(2):317–332. doi: 10.1016/0008-8749(82)90182-4. [DOI] [PubMed] [Google Scholar]
  9. Mahy B. W. Strategies of virus persistence. Br Med Bull. 1985 Jan;41(1):50–55. doi: 10.1093/oxfordjournals.bmb.a072024. [DOI] [PubMed] [Google Scholar]
  10. Mathur A., Arora K. L., Chaturvedi U. C. Congenital infection of mice with Japanese encephalitis virus. Infect Immun. 1981 Oct;34(1):26–29. doi: 10.1128/iai.34.1.26-29.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mathur A., Arora K. L., Rawat S., Chaturvedi U. C. Japanese encephalitis virus latency following congenital infection in mice. J Gen Virol. 1986 May;67(Pt 5):945–947. doi: 10.1099/0022-1317-67-5-945. [DOI] [PubMed] [Google Scholar]
  12. Mathur A., Rawat S., Chaturvedi U. C. Induction of suppressor cells in Japanese encephalitis virus infected mice. Br J Exp Pathol. 1983 Jun;64(3):336–343. [PMC free article] [PubMed] [Google Scholar]
  13. Morahan P. S., Connor J. R., Leary K. R. Viruses and the versatile macrophage. Br Med Bull. 1985 Jan;41(1):15–21. doi: 10.1093/oxfordjournals.bmb.a072017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Shanley J. D., Pesanti E. L. Murine peritoneal macrophages support murine cytomegalovirus replication. Infect Immun. 1983 Sep;41(3):1352–1359. doi: 10.1128/iai.41.3.1352-1359.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Tsuru S., Fujisawa H., Taniguchi M., Zinnaka Y., Nomoto K. Mechanism of protection during the early phase of a generalized viral infection. II. Contribution of polymorphonuclear leukocytes to protection against intravenous infection with influenza virus. J Gen Virol. 1987 Feb;68(Pt 2):419–424. doi: 10.1099/0022-1317-68-2-419. [DOI] [PubMed] [Google Scholar]
  16. Tsuru S., Kitani H., Seno M., Abe M., Zinnaka Y., Nomoto K. Mechanism of protection during the early phase of a generalized viral infection. I. Contribution of phagocytes to protection against ectromelia virus. J Gen Virol. 1983 Sep;64(Pt 9):2021–2026. doi: 10.1099/0022-1317-64-9-2021. [DOI] [PubMed] [Google Scholar]

Articles from British journal of experimental pathology are provided here courtesy of Wiley

RESOURCES