Abstract
Smooth muscle cells derived from the media of adult rat aorta were grown on elastin membranes for up to 3 weeks in cell culture. Cell degeneration was evident by the 4th day and increased with time. At the end of the experiment necrotic and calcified areas were prominent. The basic feature of cell degeneration was dissolution of myofilaments; the disintegrating cytoplasm usually contained numerous glycogen granules. Increased electron density of the mitochondrial matrix and fat globules were seen and nuclei showed dilution of chromatin or pyknosis. Abundant cellular debris, vesicular structures and microfibrils were found in the extracellular space.
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- Bierman E. L., Albers J. J. Lipoprotein uptake and degradation by cultured human arterial smooth muscle cells. Adv Exp Med Biol. 1976;67(00):437–452. doi: 10.1007/978-1-4614-4618-7_26. [DOI] [PubMed] [Google Scholar]
- Björkerud S. Cultivated human arterial smooth muscle displays heterogeneous pattern of growth and phenotypic variation. Lab Invest. 1985 Sep;53(3):303–310. [PubMed] [Google Scholar]
- Brody W. R., Angeli W. W., Kosek J. C. Histologic fate of the venous coronary artery bypass in dogs. Am J Pathol. 1972 Jan;66(1):111–130. [PMC free article] [PubMed] [Google Scholar]
- Brown B. G., Mahley R., Assmann G. Swine aortic smooth muscle in tissue culture. Some effects of purified swine lipoproteins on cell growth and morphology. Circ Res. 1976 Sep;39(3):415–424. doi: 10.1161/01.res.39.3.415. [DOI] [PubMed] [Google Scholar]
- Chait A., Ross R., Albers J. J., Bierman E. L. Platelet-derived growth factor stimulates activity of low density lipoprotein receptors. Proc Natl Acad Sci U S A. 1980 Jul;77(7):4084–4088. doi: 10.1073/pnas.77.7.4084. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cliff W. J. The aortic tunica media in aging rats. Exp Mol Pathol. 1970 Oct;13(2):172–189. doi: 10.1016/0014-4800(70)90004-3. [DOI] [PubMed] [Google Scholar]
- DIXON K. C. Deposition of globular lipid in arterial cells in relation to anoxia. Am J Pathol. 1961 Jul;39:65–74. [PMC free article] [PubMed] [Google Scholar]
- Hagler H. K., Sherwin L., Buja L. M. Effect of different methods of tissue preparation on mitochondrial inclusions of ischemic and infarcted canine myocardium: transmission and analytic electron microscopic study. Lab Invest. 1979 May;40(5):529–544. [PubMed] [Google Scholar]
- LANSING A. I., ROSENTHAL T. B., ALEX M., DEMPSEY E. W. The structure and chemical characterization of elastic fibers as revealed by elastase and by electron microscopy. Anat Rec. 1952 Dec;114(4):555–575. doi: 10.1002/ar.1091140404. [DOI] [PubMed] [Google Scholar]
- Rogers D. C. Cell contacts and smooth muscle bundle formation in tissue transplants into the anterior eye chamber. Z Zellforsch Mikrosk Anat. 1972;133(1):21–33. doi: 10.1007/BF00307065. [DOI] [PubMed] [Google Scholar]
- Ross R. The pathogenesis of atherosclerosis--an update. N Engl J Med. 1986 Feb 20;314(8):488–500. doi: 10.1056/NEJM198602203140806. [DOI] [PubMed] [Google Scholar]
- Sottiurai V. S., Kollros P., Glagov S., Zarins C. K., Mathews M. B. Morphologic alteration of cultured arterial smooth muscle cells by cyclic stretching. J Surg Res. 1983 Dec;35(6):490–497. doi: 10.1016/0022-4804(83)90038-0. [DOI] [PubMed] [Google Scholar]
- Thyberg J., Nilsson J., Palmberg L., Sjölund M. Adult human arterial smooth muscle cells in primary culture. Modulation from contractile to synthetic phenotype. Cell Tissue Res. 1985;239(1):69–74. doi: 10.1007/BF00214904. [DOI] [PubMed] [Google Scholar]
- Yamashima T., Kida S., Yamamoto S. An electron microscopic study of cerebral vasospasm with resultant myonecrosis in cases of subarachnoid haemorrhage, meningitis and trans-sylvian surgery. J Neurol. 1986 Nov;233(6):348–357. doi: 10.1007/BF00313921. [DOI] [PubMed] [Google Scholar]