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British Journal of Experimental Pathology logoLink to British Journal of Experimental Pathology
. 1988 Apr;69(2):309–319.

A monoclonal antibody (anti-L-35) which reacts with human osteoclasts and cells of the mononuclear phagocyte system.

N A Athanasou 1, P A Hall 1, A J d'Ardenne 1, J Quinn 1, J O McGee 1
PMCID: PMC2013207  PMID: 3288267

Abstract

The immunohistochemical reactivity of a monoclonal antibody, anti-L-35, on a wide range of tissues is described. Anti-L-35 showed a high specificity for known and presumptive cells of the mononuclear phagocyte system including monocytes, sinus histiocytes, tangible body macrophages, interdigitating reticulum cells, Kupffer cells, alveolar macrophages, microglia, synoviocytes and Langerhans cells. Anti-L-35 also stained osteoclasts in fetal and adult bone including osteoclast-like giant cells of the giant-cell tumour of bone. Anti-L-35 did not react with any other cell type in the tissues screened apart from renal proximal tubular epithelium and megakaryocytes. This study has shown that L-35 is not restricted to activated T-cells, as previously reported, and provides further immunohistochemical evidence that monocytes, macrophages and osteoclasts contain common cellular antigens.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beckstead J. H., Stenberg P. E., McEver R. P., Shuman M. A., Bainton D. F. Immunohistochemical localization of membrane and alpha-granule proteins in human megakaryocytes: application to plastic-embedded bone marrow biopsy specimens. Blood. 1986 Feb;67(2):285–293. [PubMed] [Google Scholar]
  2. Chambers T. J., Magnus C. J. Calcitonin alters behaviour of isolated osteoclasts. J Pathol. 1982 Jan;136(1):27–39. doi: 10.1002/path.1711360104. [DOI] [PubMed] [Google Scholar]
  3. Chambers T. J. The cellular basis of bone resorption. Clin Orthop Relat Res. 1980 Sep;(151):283–293. [PubMed] [Google Scholar]
  4. Chambers T. J. The pathobiology of the osteoclast. J Clin Pathol. 1985 Mar;38(3):241–252. doi: 10.1136/jcp.38.3.241. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Franklin W. A., Mason D. Y., Pulford K., Falini B., Bliss E., Gatter K. C., Stein H., Clarke L. C., McGee J. O. Immunohistological analysis of human mononuclear phagocytes and dendritic cells by using monoclonal antibodies. Lab Invest. 1986 Mar;54(3):322–335. [PubMed] [Google Scholar]
  6. Goldring S. R., Roelke M. S., Petrison K. K., Bhan A. K. Human giant cell tumors of bone identification and characterization of cell types. J Clin Invest. 1987 Feb;79(2):483–491. doi: 10.1172/JCI112838. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Göthlin G., Ericsson J. L. The osteoclast: review of ultrastructure, origin, and structure-function relationship. Clin Orthop Relat Res. 1976 Oct;(120):201–231. [PubMed] [Google Scholar]
  8. Horton M. A., Lewis D., McNulty K., Pringle J. A., Chambers T. J. Human fetal osteoclasts fail to express macrophage antigens. Br J Exp Pathol. 1985 Feb;66(1):103–108. [PMC free article] [PubMed] [Google Scholar]
  9. Horton M. A., Lewis D., McNulty K., Pringle J. A., Chambers T. J. Monoclonal antibodies to osteoclastomas (giant cell bone tumors): definition of osteoclast-specific cellular antigens. Cancer Res. 1985 Nov;45(11 Pt 2):5663–5669. [PubMed] [Google Scholar]
  10. Horton M. A., Rimmer E. F., Moore A., Chambers T. J. On the origin of the osteoclast: the cell surface phenotype of rodent osteoclasts. Calcif Tissue Int. 1985 Jan;37(1):46–50. doi: 10.1007/BF02557678. [DOI] [PubMed] [Google Scholar]
  11. Lasser A. The mononuclear phagocytic system: a review. Hum Pathol. 1983 Feb;14(2):108–126. doi: 10.1016/s0046-8177(83)80239-1. [DOI] [PubMed] [Google Scholar]
  12. Maino V. C., Janszen M. E. Monoclonal antibodies, L-35 and L-36, define novel T cell activation antigens. J Immunol. 1986 Nov 15;137(10):3093–3099. [PubMed] [Google Scholar]
  13. Marks S. C., Jr The origin of osteoclasts: evidence, clinical implications and investigative challenges of an extra-skeletal source. J Oral Pathol. 1983 Aug;12(4):226–256. doi: 10.1111/j.1600-0714.1983.tb00337.x. [DOI] [PubMed] [Google Scholar]
  14. Owen M. Histogenesis of bone cells. Calcif Tissue Res. 1978 Aug 18;25(3):205–207. doi: 10.1007/BF02010770. [DOI] [PubMed] [Google Scholar]
  15. Sminia T., Dijkstra C. D. The origin of osteoclasts: an immunohistochemical study on macrophages and osteoclasts in embryonic rat bone. Calcif Tissue Int. 1986 Oct;39(4):263–266. doi: 10.1007/BF02555216. [DOI] [PubMed] [Google Scholar]
  16. Sutton J. S., Weiss L. Transformation of monocytes in tissue culture into macrophages, epithelioid cells, and multinucleated giant cells. An electron microscope study. J Cell Biol. 1966 Feb;28(2):303–332. doi: 10.1083/jcb.28.2.303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Thorbecke G. J., Silberberg-Sinakin I., Flotte T. J. Langerhans cells as macrophages in skin and lymphoid organs. J Invest Dermatol. 1980 Jul;75(1):32–43. doi: 10.1111/1523-1747.ep12521083. [DOI] [PubMed] [Google Scholar]

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