Abstract
The interactions of cultured bovine aortic and human umbilical or saphenous vein endothelium with cultured fibroblasts or smooth muscle cells were studied using light microscopy, scanning electron microscopy, and a radioisotope adhesion assay. (1) Resuspended fibroblasts or smooth muscle cells readily 'overgrew' confluent endothelial monolayers under static culture conditions, but not when cultures were exposed to a continuously stirred medium. (2) Exposure of cultured endothelial or smooth muscle cells to a moderately alkaline environment alters the disposition of pericellular concanavalin. A positive extracellular material. This does not affect the initial adhesion of endothelial or smooth muscle cells, but does affect cell spreading. (3) Endothelial adhesion to cultured smooth muscle cells involves both adhesion and spreading. Recently subcultured or rapidly proliferating smooth muscle cells support initial adhesion, but not spreading. Spreading appears to require the establishment of a suitable extracellular matrix, and this is inhibited both by a flowing medium and by an alkaline extracellular environment.
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- Clowes A. W., Gown A. M., Hanson S. R., Reidy M. A. Mechanisms of arterial graft failure. 1. Role of cellular proliferation in early healing of PTFE prostheses. Am J Pathol. 1985 Jan;118(1):43–54. [PMC free article] [PubMed] [Google Scholar]
- FLOREY H. W., GREER S. J., KISER J., POOLE J. C., TELANDER R., WERTHESSEN N. T. The development of the pseudointima lining fabric grafts of the aorta. Br J Exp Pathol. 1962 Dec;43:655–660. [PMC free article] [PubMed] [Google Scholar]
- Fry D. L. Acute vascular endothelial changes associated with increased blood velocity gradients. Circ Res. 1968 Feb;22(2):165–197. doi: 10.1161/01.res.22.2.165. [DOI] [PubMed] [Google Scholar]
- Gajdusek C. M., Schwartz S. M. Technique for cloning bovine aortic endothelial cells. In Vitro. 1983 May;19(5):394–402. doi: 10.1007/BF02619556. [DOI] [PubMed] [Google Scholar]
- Heydorn W. H., Geasling J. W., Moores W. Y., Lollini L. O., Gomez A. C. Changes in the manufacture of expanded microporous polytetrafluoroethylene: effects on patency and histological behavior when used to replace the superior vena cava. Ann Thorac Surg. 1979 Feb;27(2):173–177. doi: 10.1016/s0003-4975(10)63263-8. [DOI] [PubMed] [Google Scholar]
- Kaiser N., Vlodavsky I., Tur-Sinai A., Fuks Z., Cerasi E. Insulin binding and degradation in vascular endothelial cells: modulation by cell growth and culture organization. Endocrinology. 1983 Jul;113(1):228–234. doi: 10.1210/endo-113-1-228. [DOI] [PubMed] [Google Scholar]
- POOLE J. C., SANDERS A. G., FLOREY H. W. The regeneration of aortic endothelium. J Pathol Bacteriol. 1958 Jan;75(1):133–143. doi: 10.1002/path.1700750116. [DOI] [PubMed] [Google Scholar]
- Ramsay M. M., Walker L. N., Bowyer D. E. Narrow superficial injury to rabbit aortic endothelium. The healing process as observed by scanning electron microscopy. Atherosclerosis. 1982 Jun;43(2-3):233–243. doi: 10.1016/0021-9150(82)90025-9. [DOI] [PubMed] [Google Scholar]
- TERASIMA T., TOLMACH L. J. Growth and nucleic acid synthesis in synchronously dividing populations of HeLa cells. Exp Cell Res. 1963 Apr;30:344–362. doi: 10.1016/0014-4827(63)90306-9. [DOI] [PubMed] [Google Scholar]