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British Journal of Experimental Pathology logoLink to British Journal of Experimental Pathology
. 1988 Apr;69(2):245–254.

Host defence mechanisms in the bladder. I. Role of mechanical factors.

G Harrison 1, J Cornish 1, M A Vanderwee 1, T E Miller 1
PMCID: PMC2013219  PMID: 3288266

Abstract

This study evaluated the contribution of host defence mechanisms to bacterial clearance from the urinary bladder using an animal model in which rats were infected with Escherichia coli. Factors studied included the effect of hydration status, induced ultrastructural changes to the surface of the bladder mucosa, and the relevance of bacterial replication. Clearance was divided into two phases, primary (0-4 h), and secondary (4-24 h). Ninety-nine per cent of Escherichia coli 075 was cleared during the primary phase from normal, dehydrated and polyuric animals and 93% from anuric animals. Clearance was shown to be dependent on the presence of viable tissue. Bacterial numbers continued to decrease during the secondary phase in normal and dehydrated animals but increased in polyuric and anuric groups. No such rise occurred when rats were inoculated with Escherichia coli E/2/64, a non-replicating mutant. Evidence of ultrastructural changes to the bladder associated with impaired antibacterial properties was found in polyuric and anuric animals. Clearance of particulate matter (killed Candida albicans) however was unaffected by mucus disruption. The study has shown that the clearance of microorganisms from the bladder was unrelated to the voided volume, but is closely associated with the antibacterial activity of the mucosal surface.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Asscher A. W., Sussman M., Waters W. E., Davis R. H., Chick S. Urine as a medium for bacterial growth. Lancet. 1966 Nov 12;2(7472):1037–1041. doi: 10.1016/s0140-6736(66)92023-x. [DOI] [PubMed] [Google Scholar]
  2. COX C. E., HINMAN F., Jr Experiments with induced bacteriuria, vesical emptying and bacterial growth on the mechanism of bladder defense to infection. J Urol. 1961 Dec;86:739–748. doi: 10.1016/S0022-5347(17)65257-1. [DOI] [PubMed] [Google Scholar]
  3. Cobbs C. G., Kaye D. Antibacterial mechanisms in the urinary bladder. Yale J Biol Med. 1967 Oct;40(2):93–108. [PMC free article] [PubMed] [Google Scholar]
  4. Cornish J., Vanderwee M., Miller T. Mucus stabilization in the urinary bladder. Br J Exp Pathol. 1987 Jun;68(3):369–375. [PMC free article] [PubMed] [Google Scholar]
  5. Davis C. P., Avots-Avotins A. E. Evaluation of bacterial attachment to acid treated bladder epithelium. Scan Electron Microsc. 1982;(Pt 2):825–830. [PubMed] [Google Scholar]
  6. Hand W. L., Smith J. W., Sanford J. P. The antibacterial effect of normal and infected urinary bladder. J Lab Clin Med. 1971 Apr;77(4):605–615. [PubMed] [Google Scholar]
  7. Hooke A. M., Oeschger M. P., Zeligs B. J., Bellanti J. A. Ideal target organism for quantitative bactericidal assays. Infect Immun. 1978 May;20(2):406–411. doi: 10.1128/iai.20.2.406-411.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kaye D. Antibacterial activity of human urine. J Clin Invest. 1968 Oct;47(10):2374–2390. doi: 10.1172/JCI105921. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. MEHROTRA R. M. An experimental study of the vesical circulation during distension and in cystitis. J Pathol Bacteriol. 1953 Jul;66(1):79–89. doi: 10.1002/path.1700660111. [DOI] [PubMed] [Google Scholar]
  10. Miller T. E., Findon G., Cawley S. Cellular basis of host defence in pyelonephritis. I. Chronic infection. Br J Exp Pathol. 1986 Feb;67(1):13–23. [PMC free article] [PubMed] [Google Scholar]
  11. Mulholland S. G., Foster E. A., Paquin A. J., Jr, Gillenwater J. Y. The effect of rabbit vesical mucosa on bacterial growth. Invest Urol. 1969 May;6(6):593–604. [PubMed] [Google Scholar]
  12. Norden C. W., Green G. M., Kass E. H. Antibacterial mechanisms of the urinary bladder. J Clin Invest. 1968 Dec;47(12):2689–2700. doi: 10.1172/JCI105952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. O'Grady F., Cattell W. R. Kinetics of urinary tract infection. II. The bladder. Br J Urol. 1966 Apr;38(2):156–162. doi: 10.1111/j.1464-410x.1966.tb09694.x. [DOI] [PubMed] [Google Scholar]
  14. O'Grady F. Urinary tract infection in women. J R Coll Physicians Lond. 1979 Apr;13(2):70–73. [PMC free article] [PubMed] [Google Scholar]
  15. Orikasa S., Hinman F., Jr Reaction of the vesical wall to bacterial penetration: resistance to attachment, desquamation, and leukocytic activity. Invest Urol. 1977 Nov;15(3):185–193. [PubMed] [Google Scholar]
  16. Parsons C. L., Shrom S. H., Hanno P. M., Mulholland S. G. Bladder surface mucin. Examination of possible mechanisms for its antibacterial effect. Invest Urol. 1978 Nov;16(3):196–200. [PubMed] [Google Scholar]

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