Abstract
Rats were subjected to 60% partial hepatectomy. In the immediate postoperative period, there was a small decline in plasma fibronectin concentrations, which was normalized already 12 h post-operatively. Metabolic labelling of fibronectin with 35S-methionine showed that the rate of incorporation of label was unchanged after partial hepatectomy. However, when the abdominal viscera were excluded from the circulation, no incorporation of label was found. These results show that the rate of fibronectin biosynthesis has the rapid regulation characteristics of an acute phase reactant. Peripheral tissues do not contribute significantly to the plasma fibronectin concentration. Intra- or retro-peritoneal viscera, most likely the liver, are responsible for the synthesis of rat plasma fibronectin.
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Selected References
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- Ahlgren T., Berghem L., Jarstrand C., Lindquist L. Plasma fibronectin is initially decreased during septicemia. Scand J Infect Dis. 1985;17(1):107–112. doi: 10.3109/00365548509070429. [DOI] [PubMed] [Google Scholar]
- Aronsen K. F., Ekelund G., Kindmark C. O., Laurell C. B. Sequential changes of plasma proteins after surgical trauma. Scand J Clin Lab Invest Suppl. 1972;124:127–136. doi: 10.3109/00365517209102760. [DOI] [PubMed] [Google Scholar]
- Deno D. C., McCafferty M. H., Saba T. M., Blumenstock F. A. Mechanism of acute depletion of plasma fibronectin following thermal injury in rats. Appearance of a gelatinlike ligand in plasma. J Clin Invest. 1984 Jan;73(1):20–34. doi: 10.1172/JCI111191. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Deutsch D. G., Mertz E. T. Plasminogen: purification from human plasma by affinity chromatography. Science. 1970 Dec 4;170(3962):1095–1096. doi: 10.1126/science.170.3962.1095. [DOI] [PubMed] [Google Scholar]
- Gluud C., Dejgaard A., Clemmensen I. Plasma fibronectin concentrations in patients with liver diseases. Scand J Clin Lab Invest. 1983 Oct;43(6):533–537. doi: 10.1080/00365518309168441. [DOI] [PubMed] [Google Scholar]
- Kaplan J. E., Molnar J., Saba T. M., Allen C. Comparative disappearance and localization of isotopically labeled opsonic protein and soluble albumin following surgical trauma. J Reticuloendothel Soc. 1976 Nov;20(5):375–384. [PubMed] [Google Scholar]
- Mosesson M. W., Amrani D. L. The structure and biologic activities of plasma fibronectin. Blood. 1980 Aug;56(2):145–158. [PubMed] [Google Scholar]
- Owens M. R., Cimino C. D. Synthesis of fibronectin by the isolated perfused rat liver. Blood. 1982 Jun;59(6):1305–1309. [PubMed] [Google Scholar]
- Parent J. B., Bauer H. C., Olden K. Three secretory rates in human hepatoma cells. Biochim Biophys Acta. 1985 Jul 30;846(1):44–50. doi: 10.1016/0167-4889(85)90108-9. [DOI] [PubMed] [Google Scholar]
- Pussell B. A., Peake P. W., Brown M. A., Charlesworth J. A. Human fibronectin metabolism. J Clin Invest. 1985 Jul;76(1):143–148. doi: 10.1172/JCI111937. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Snyder E. L., Barash P. G., Mosher D. F., Walter S. D. Plasma fibronectin level and clinical status in cardiac surgery patients. J Lab Clin Med. 1983 Dec;102(6):881–889. [PubMed] [Google Scholar]