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British Journal of Experimental Pathology logoLink to British Journal of Experimental Pathology
. 1987 Jun;68(3):319–330.

Metabolic and physical changes during calcium paradox induced in the rat heart.

H Kanaide, H Meno, M Nakamura
PMCID: PMC2013259  PMID: 3620328

Abstract

Early changes in metabolic and physical properties were determined in rat hearts during calcium paradox. Calcium paradox was induced under constant perfusion pressure (60 mmHg) or constant coronary flow rate (9.8 ml/min). Within 30 s after calcium repletion, in either case, NADH increased, despite a decrease in ATP and increases in ADP and AMP. Surface spectrophotometry showed a deoxygenation of the myoglobin, thereby indicating myocardial oxygen depletion. These changes were predominant under conditions of constant pressure perfusion. In association with a rapid development of contracture, there were also a reduction in coronary flow (18%) in constant pressure perfusion, and an increase in perfusion pressure (208%) under constant flow perfusion. Thus, tissue deoxygenation has to be given due attention in the early development of calcium paradox, particularly in case of a constant pressure perfusion. Under constant flow perfusion, the physical stress due to high pressure perfusion against contracture may play an important role in the development of calcium paradox. This may be the first reported evidence for tissue anoxia in calcium paradox.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ashraf M. Correlative studies on sarcolemmal ultrastructure, permeability, and loss of intracellular enzymes in the isolated heart perfused with calcium-free medium. Am J Pathol. 1979 Nov;97(2):411–432. [PMC free article] [PubMed] [Google Scholar]
  2. Bionk A. B., Ruigrok T. J., Maas A. H., Zimmerman A. N. Changes in high-energy phosphate compounds of isolated rat hearts during Ca2+-free perfusion and reperfusion with Ca2+. J Mol Cell Cardiol. 1976 Dec;8(12):973–979. doi: 10.1016/0022-2828(76)90078-x. [DOI] [PubMed] [Google Scholar]
  3. Frank J. S. Ca depletion of the sarcolemma--ultrastructural changes. Eur Heart J. 1983 Dec;4 (Suppl H):23–27. doi: 10.1093/eurheartj/4.suppl_h.23. [DOI] [PubMed] [Google Scholar]
  4. Ganote C. E., Nayler W. G. Contracture and the calcium paradox. J Mol Cell Cardiol. 1985 Aug;17(8):733–745. doi: 10.1016/s0022-2828(85)80035-3. [DOI] [PubMed] [Google Scholar]
  5. Ganote C. E., Sims M. A. Physical stress-mediated enzyme release from calcium-deficient hearts. J Mol Cell Cardiol. 1983 Jul;15(7):421–429. doi: 10.1016/0022-2828(83)90262-6. [DOI] [PubMed] [Google Scholar]
  6. Grinwald P. M., Nayler W. G. Calcium entry in the calcium paradox. J Mol Cell Cardiol. 1981 Oct;13(10):867–880. doi: 10.1016/0022-2828(81)90286-8. [DOI] [PubMed] [Google Scholar]
  7. Koomen J. M., Jager L. P., van Noordwijk J. Effects of perfusion pressure on coronary flow, myocardial Ca2+-washout, and the occurrence of calcium paradox in isolated perfused rat heart ventricles. Basic Res Cardiol. 1980 Mar-Apr;75(2):318–327. doi: 10.1007/BF01907580. [DOI] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Makino N., Kanaide H., Yoshimura R., Nakamura M. Myoglobin oxygenation remains constant during the cardiac cycle. Am J Physiol. 1983 Aug;245(2):H237–H243. doi: 10.1152/ajpheart.1983.245.2.H237. [DOI] [PubMed] [Google Scholar]
  10. Nayler W. G., Elz J. S., Perry S. E., Daly M. J. The biochemistry of uncontrolled calcium entry. Eur Heart J. 1983 Dec;4 (Suppl H):29–41. doi: 10.1093/eurheartj/4.suppl_h.29. [DOI] [PubMed] [Google Scholar]
  11. Owen C. S., Wilson D. F. Control of respiration by the mitochondrial phosphorylation state. Arch Biochem Biophys. 1974 Apr 2;161(2):581–591. doi: 10.1016/0003-9861(74)90341-5. [DOI] [PubMed] [Google Scholar]
  12. Randall W. C., Rinkema L. E., Jones S. B., Moran J. F., Brynjolfsson G. Functional characterization of atrial pacemaker activity. Am J Physiol. 1982 Jan;242(1):H98–106. doi: 10.1152/ajpheart.1982.242.1.H98. [DOI] [PubMed] [Google Scholar]
  13. Rosalki S. B. An improved procedure for serum creatine phosphokinase determination. J Lab Clin Med. 1967 Apr;69(4):696–705. [PubMed] [Google Scholar]
  14. Ruigrok T. J., Boink A. B., Spies F., Blok F. J., Maas A. H., Zimmerman A. N. Energy dependence of the calcium paradox. J Mol Cell Cardiol. 1978 Nov;10(11):991–1002. doi: 10.1016/0022-2828(78)90395-4. [DOI] [PubMed] [Google Scholar]
  15. Zimmerman A. N., Hülsmann W. C. Paradoxical influence of calcium ions on the permeability of the cell membranes of the isolated rat heart. Nature. 1966 Aug 6;211(5049):646–647. doi: 10.1038/211646a0. [DOI] [PubMed] [Google Scholar]

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