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. 1994 Feb;60(2):434–446. doi: 10.1128/aem.60.2.434-446.1994

Multicellular Organization in a Degradative Biofilm Community

G M Wolfaardt 1,2, J R Lawrence 1,*, R D Robarts 1, S J Caldwell 3, D E Caldwell 2
PMCID: PMC201331  PMID: 16349173

Abstract

Diclofop methyl, a commercial herbicide, was used as the sole carbon source to cultivate diclofop-degrading biofilms in continuous-flow slide culture. The biofilms were analyzed by using scanning confocal laser microscopy and image analysis. Spatial relationships among members of the community were distinctive to diclofop-grown biofilms. These relationships did not develop when the biofilms were grown on more labile substrates but were conserved when the biofilms were cultivated with other chlorinated ring compounds. The structures included conical bacterial consortia rising to 30 μm above the surrounding biofilm, grape-like clusters of cocci embedded in a matrix of perpendicularly oriented bacilli, and other highly specific patterns of intra- and intergeneric cellular coaggregation and growth. These unique consortial relationships indicated that syntrophic interactions may be necessary for optimal degradation of diclofop methyl and other chlorinated ring compounds.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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