Abstract
A previous study reported the isolation of two mutants (LE1 and LE2) of the plant pathogenic fungus Nectria haematococca (anamorph, Fusarium solani f. sp. cucurbitae) with macroconidia with reduced ability to adhere (Att-) to zucchini fruits and polystyrene. The adhesion-reduced-phenotype in LE1 and LE2 macroconidia is temperature sensitive and dependent on the concentration of nutrients. Classical genetic analysis of progeny derived from LE1 identified a mutation in a genetic locus, named Att1. The 90-kDa glycoprotein and macroconidial tip mucilage which were previously associated with the development of adhesion competence in Att+ macroconidia are specifically associated with macroconidia; neither is produced on microconidia, which are relatively nonadherent. However, macroconidia of both Att+ and Att- strains produce the 90-kDa glycoprotein and the macroconidial tip mucilage.
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- Dubray G., Bezard G. A highly sensitive periodic acid-silver stain for 1,2-diol groups of glycoproteins and polysaccharides in polyacrylamide gels. Anal Biochem. 1982 Jan 15;119(2):325–329. doi: 10.1016/0003-2697(82)90593-0. [DOI] [PubMed] [Google Scholar]
- Hamer J. E., Howard R. J., Chumley F. G., Valent B. A mechanism for surface attachment in spores of a plant pathogenic fungus. Science. 1988 Jan 15;239(4837):288–290. doi: 10.1126/science.239.4837.288. [DOI] [PubMed] [Google Scholar]
- Jones M. J., Epstein L. Adhesion of Macroconidia to the Plant Surface and Virulence of Nectria haematococca. Appl Environ Microbiol. 1990 Dec;56(12):3772–3778. doi: 10.1128/aem.56.12.3772-3778.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Oakley B. R., Kirsch D. R., Morris N. R. A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels. Anal Biochem. 1980 Jul 1;105(2):361–363. doi: 10.1016/0003-2697(80)90470-4. [DOI] [PubMed] [Google Scholar]