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. 1994 Mar;60(3):908–912. doi: 10.1128/aem.60.3.908-912.1994

Distribution of tetracycline resistance determinants among gram-negative bacteria isolated from polluted and unpolluted marine sediments.

S R Andersen 1, R A Sandaa 1
PMCID: PMC201409  PMID: 8161183

Abstract

Tetracycline-resistant gram-negative bacteria were isolated from four different marine sediments in Scandinavia and analyzed with DNA probes for the determinant classes A to E. Colony hybridizations of 429 isolates revealed that class E is the dominating resistance determinant in these marine sediments. Comparison of fecally polluted and unpolluted sediments showed few determinant classes in unpolluted sediment and a complex composition of several determinant classes in polluted sediment. Total DNA extraction and analysis with DNA probes for determinant classes A to E resulted in no hybridization signal, because of the low number of gram-negative tetracycline-resistant bacteria. Identification of class E isolates revealed that this determinant is present not only in Aeromonas hydrophila, Escherichia coli, and Vibrio salmonicida but also in additional strains.

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Selected References

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  1. Aoki T. Drug-resistant plasmids from fish pathogens. Microbiol Sci. 1988 Jul;5(7):219–223. [PubMed] [Google Scholar]
  2. Aoki T., Satoh T., Kitao T. New tetracycline resistance determinant on R plasmids from Vibrio anguillarum. Antimicrob Agents Chemother. 1987 Sep;31(9):1446–1449. doi: 10.1128/aac.31.9.1446. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Aoki T., Takahashi A. Class D tetracycline resistance determinants of R plasmids from the fish pathogens Aeromonas hydrophila, Edwardsiella tarda, and Pasteurella piscicida. Antimicrob Agents Chemother. 1987 Aug;31(8):1278–1280. doi: 10.1128/aac.31.8.1278. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Baya A. M., Brayton P. R., Brown V. L., Grimes D. J., Russek-Cohen E., Colwell R. R. Coincident plasmids and antimicrobial resistance in marine bacteria isolated from polluted and unpolluted Atlantic Ocean samples. Appl Environ Microbiol. 1986 Jun;51(6):1285–1292. doi: 10.1128/aem.51.6.1285-1292.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Buck J. D. Nonstaining (KOH) method for determination of gram reactions of marine bacteria. Appl Environ Microbiol. 1982 Oct;44(4):992–993. doi: 10.1128/aem.44.4.992-993.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Curiale M. S., Levy S. B. Two complementation groups mediate tetracycline resistance determined by Tn10. J Bacteriol. 1982 Jul;151(1):209–215. doi: 10.1128/jb.151.1.209-215.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. DePaola A., Flynn P. A., McPhearson R. M., Levy S. B. Phenotypic and genotypic characterization of tetracycline- and oxytetracycline-resistant Aeromonas hydrophila from cultured channel catfish (Ictalurus punctatus) and their environments. Appl Environ Microbiol. 1988 Jul;54(7):1861–1863. doi: 10.1128/aem.54.7.1861-1863.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gerba C. P., McLeod J. S. Effect of sediments on the survival of Escherichia coli in marine waters. Appl Environ Microbiol. 1976 Jul;32(1):114–120. doi: 10.1128/aem.32.1.114-120.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Grunstein M., Hogness D. S. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961–3965. doi: 10.1073/pnas.72.10.3961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lee C., Langlois B. E., Dawson K. A. Detection of tetracycline resistance determinants in pig isolates from three herds with different histories of antimicrobial agent exposure. Appl Environ Microbiol. 1993 May;59(5):1467–1472. doi: 10.1128/aem.59.5.1467-1472.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Marshall B., Morrissey S., Flynn P., Levy S. B. A new tetracycline-resistance determinant, class E, isolated from Enterobacteriaceae. Gene. 1986;50(1-3):111–117. doi: 10.1016/0378-1119(86)90315-x. [DOI] [PubMed] [Google Scholar]
  12. Marshall B., Tachibana C., Levy S. B. Frequency of tetracycline resistance determinant classes among lactose-fermenting coliforms. Antimicrob Agents Chemother. 1983 Dec;24(6):835–840. doi: 10.1128/aac.24.6.835. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mendez B., Tachibana C., Levy S. B. Heterogeneity of tetracycline resistance determinants. Plasmid. 1980 Mar;3(2):99–108. doi: 10.1016/0147-619x(80)90101-8. [DOI] [PubMed] [Google Scholar]
  14. Park B. H., Hendricks M., Malamy M. H., Tally F. P., Levy S. B. Cryptic tetracycline resistance determinant (class F) from Bacteroides fragilis mediates resistance in Escherichia coli by actively reducing tetracycline accumulation. Antimicrob Agents Chemother. 1987 Nov;31(11):1739–1743. doi: 10.1128/aac.31.11.1739. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Paul J. H., Frischer M. E., Thurmond J. M. Gene transfer in marine water column and sediment microcosms by natural plasmid transformation. Appl Environ Microbiol. 1991 May;57(5):1509–1515. doi: 10.1128/aem.57.5.1509-1515.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Roszak D. B., Colwell R. R. Metabolic activity of bacterial cells enumerated by direct viable count. Appl Environ Microbiol. 1987 Dec;53(12):2889–2893. doi: 10.1128/aem.53.12.2889-2893.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sizemore R. K., Colwell R. R. Plasmids carried by antibiotic-resistant marine bacteria. Antimicrob Agents Chemother. 1977 Sep;12(3):373–382. doi: 10.1128/aac.12.3.373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Stewart G. J., Sinigalliano C. D. Detection of horizontal gene transfer by natural transformation in native and introduced species of bacteria in marine and synthetic sediments. Appl Environ Microbiol. 1990 Jun;56(6):1818–1824. doi: 10.1128/aem.56.6.1818-1824.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sørum H., Roberts M. C., Crosa J. H. Identification and cloning of a tetracycline resistance gene from the fish pathogen Vibrio salmonicida. Antimicrob Agents Chemother. 1992 Mar;36(3):611–615. doi: 10.1128/aac.36.3.611. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Torsvik V., Goksøyr J., Daae F. L. High diversity in DNA of soil bacteria. Appl Environ Microbiol. 1990 Mar;56(3):782–787. doi: 10.1128/aem.56.3.782-787.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]

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