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. 1994 Mar;60(3):966–972. doi: 10.1128/aem.60.3.966-972.1994

Comparative biochemical and genetic analysis of naphthalene degradation among Pseudomonas stutzeri strains.

R A Rosselló-Mora 1, J Lalucat 1, E García-Valdés 1
PMCID: PMC201418  PMID: 8161187

Abstract

Of a 49-strain collection of Pseudomonas stutzeri species, 11 isolates were able to degrade naphthalene and 1 isolate was able to use m- and p-toluate as sole carbon and energy sources. Of these 12 strains, 10 shared a highly homologous set of naphthalene catabolic genes, even though they belong to four different genomovars. These genes differed from those present in plasmid NAH7. In only one of these degraders could a plasmid-encoded pathway be demonstrated, and a chromosome-encoded pathway is proposed for the remaining strains. meta cleavage of catechol was only observed in those strains able to metabolize alkyl derivatives of catechol.

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Selected References

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  1. Baggi G., Barbieri P., Galli E., Tollari S. Isolation of a Pseudomonas stutzeri strain that degrades o-xylene. Appl Environ Microbiol. 1987 Sep;53(9):2129–2132. doi: 10.1128/aem.53.9.2129-2132.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barnsley E. A. The induction of the enzymes of naphthalene metabolism in pseudomonads by salicylate and 2-aminobenzoate. J Gen Microbiol. 1975 May;88(1):193–196. doi: 10.1099/00221287-88-1-193. [DOI] [PubMed] [Google Scholar]
  3. Benjamin R. C., Voss J. A., Kunz D. A. Nucleotide sequence of xylE from the TOL pDK1 plasmid and structural comparison with isofunctional catechol-2,3-dioxygenase genes from TOL, pWW0 and NAH7. J Bacteriol. 1991 Apr;173(8):2724–2728. doi: 10.1128/jb.173.8.2724-2728.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chatfield L. K., Williams P. A. Naturally occurring TOL plasmids in Pseudomonas strains carry either two homologous or two nonhomologous catechol 2,3-oxygenase genes. J Bacteriol. 1986 Nov;168(2):878–885. doi: 10.1128/jb.168.2.878-885.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chatterjee D. K., Chakrabarty A. M. Genetic homology between independently isolated chlorobenzoate-degradative plasmids. J Bacteriol. 1983 Jan;153(1):532–534. doi: 10.1128/jb.153.1.532-534.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Connors M. A., Barnsley E. A. Naphthalene plasmids in pseudomonads. J Bacteriol. 1982 Mar;149(3):1096–1101. doi: 10.1128/jb.149.3.1096-1101.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cubo M. T., Buendia-Claveria A. M., Beringer J. E., Ruiz-Sainz J. E. Melanin production by Rhizobium strains. Appl Environ Microbiol. 1988 Jul;54(7):1812–1817. doi: 10.1128/aem.54.7.1812-1817.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. DAGLEY S., CHAPMAN P. J., GIBSON D. T., WOOD J. M. DEGRADATION OF THE BENZENE NUCLEUS BY BACTERIA. Nature. 1964 May 23;202:775–778. doi: 10.1038/202775a0. [DOI] [PubMed] [Google Scholar]
  9. Eaton R. W., Chapman P. J. Bacterial metabolism of naphthalene: construction and use of recombinant bacteria to study ring cleavage of 1,2-dihydroxynaphthalene and subsequent reactions. J Bacteriol. 1992 Dec;174(23):7542–7554. doi: 10.1128/jb.174.23.7542-7554.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Feist C. F., Hegeman G. D. Phenol and benzoate metabolism by Pseudomonas putida: regulation of tangential pathways. J Bacteriol. 1969 Nov;100(2):869–877. doi: 10.1128/jb.100.2.869-877.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. García-Valdés E., Cozar E., Rotger R., Lalucat J., Ursing J. New naphthalene-degrading marine Pseudomonas strains. Appl Environ Microbiol. 1988 Oct;54(10):2478–2485. doi: 10.1128/aem.54.10.2478-2485.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hegeman G. D. Synthesis of the enzymes of the mandelate pathway by Pseudomonas putida. I. Synthesis of enzymes by the wild type. J Bacteriol. 1966 Mar;91(3):1140–1154. doi: 10.1128/jb.91.3.1140-1154.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ish-Horowicz D., Burke J. F. Rapid and efficient cosmid cloning. Nucleic Acids Res. 1981 Jul 10;9(13):2989–2998. doi: 10.1093/nar/9.13.2989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kado C. I., Liu S. T. Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol. 1981 Mar;145(3):1365–1373. doi: 10.1128/jb.145.3.1365-1373.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kochetkov V. V., Boronin A. M. Sravnitel'noe izuchenie plazmid, kontroliruiushchikh biodegradatsiiu naftalina kul'turoi Pseudomonas. Mikrobiologiia. 1984 Jul-Aug;53(4):639–644. [PubMed] [Google Scholar]
  16. Kurkela S., Lehväslaiho H., Palva E. T., Teeri T. H. Cloning, nucleotide sequence and characterization of genes encoding naphthalene dioxygenase of Pseudomonas putida strain NCIB9816. Gene. 1988 Dec 20;73(2):355–362. doi: 10.1016/0378-1119(88)90500-8. [DOI] [PubMed] [Google Scholar]
  17. Lind E., Ursing J. Clinical strains of Enterobacter agglomerans (synonyms: Erwinia herbicola, Erwinia milletiae) identified by DNA-DNA-hybridization. Acta Pathol Microbiol Immunol Scand B. 1986 Aug;94(4):205–213. doi: 10.1111/j.1699-0463.1986.tb03043.x. [DOI] [PubMed] [Google Scholar]
  18. Lorenz M. G., Wackernagel W. Natural genetic transformation of Pseudomonas stutzeri by sand-adsorbed DNA. Arch Microbiol. 1990;154(4):380–385. doi: 10.1007/BF00276535. [DOI] [PubMed] [Google Scholar]
  19. Nakazawa T., Yokota T. Benzoate metabolism in Pseudomonas putida(arvilla) mt-2: demonstration of two benzoate pathways. J Bacteriol. 1973 Jul;115(1):262–267. doi: 10.1128/jb.115.1.262-267.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. ORNSTON L. N., STANIER R. Y. MECHANISM OF BETA-KETOADIPATE FORMATION BY BACTERIA. Nature. 1964 Dec 26;204:1279–1283. doi: 10.1038/2041279a0. [DOI] [PubMed] [Google Scholar]
  21. Obradors N., Aguilar J. Efficient biodegradation of high-molecular-weight polyethylene glycols by pure cultures of Pseudomonas stutzeri. Appl Environ Microbiol. 1991 Aug;57(8):2383–2388. doi: 10.1128/aem.57.8.2383-2388.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Ogunseitan O. A., Delgado I. L., Tsai Y. L., Olson B. H. Effect of 2-hydroxybenzoate on the maintenance of naphthalene-degrading pseudomonads in seeded and unseeded soil. Appl Environ Microbiol. 1991 Oct;57(10):2873–2879. doi: 10.1128/aem.57.10.2873-2879.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Rosenberg C., Casse-Delbart F., Dusha I., David M., Boucher C. Megaplasmids in the plant-associated bacteria Rhizobium meliloti and Pseudomonas solanacearum. J Bacteriol. 1982 Apr;150(1):402–406. doi: 10.1128/jb.150.1.402-406.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Schell M. A. Transcriptional control of the nah and sal hydrocarbon-degradation operons by the nahR gene product. Gene. 1985;36(3):301–309. doi: 10.1016/0378-1119(85)90185-4. [DOI] [PubMed] [Google Scholar]
  25. Shamsuzzaman K. M., Barnsley E. A. The regulation of naphthalene metabolism in pseudomonads. Biochem Biophys Res Commun. 1974 Sep 23;60(2):582–589. doi: 10.1016/0006-291x(74)90280-0. [DOI] [PubMed] [Google Scholar]
  26. Shamsuzzaman K. M., Barnsley E. A. The regulation of naphthalene oxygenase in pseudomonads. J Gen Microbiol. 1974 Jul;83(0):165–170. doi: 10.1099/00221287-83-1-165. [DOI] [PubMed] [Google Scholar]
  27. Simon M. J., Osslund T. D., Saunders R., Ensley B. D., Suggs S., Harcourt A., Suen W. C., Cruden D. L., Gibson D. T., Zylstra G. J. Sequences of genes encoding naphthalene dioxygenase in Pseudomonas putida strains G7 and NCIB 9816-4. Gene. 1993 May 15;127(1):31–37. doi: 10.1016/0378-1119(93)90613-8. [DOI] [PubMed] [Google Scholar]
  28. Sinclair M. I., Holloway B. W. Chromosomal insertion of TOL transposons in Pseudomonas aeruginosa PAO. J Gen Microbiol. 1991 May;137(5):1111–1120. doi: 10.1099/00221287-137-5-1111. [DOI] [PubMed] [Google Scholar]
  29. Smith P. K., Krohn R. I., Hermanson G. T., Mallia A. K., Gartner F. H., Provenzano M. D., Fujimoto E. K., Goeke N. M., Olson B. J., Klenk D. C. Measurement of protein using bicinchoninic acid. Anal Biochem. 1985 Oct;150(1):76–85. doi: 10.1016/0003-2697(85)90442-7. [DOI] [PubMed] [Google Scholar]
  30. Starovoitov I. I. Reguliatsiia sinteza kliuchevykh fermentov katabolizma naftalina u Pseudomonas putida i Pseudomonas fluorescens, nesushchikh plazmidy biodegradatsii NAH, pBS3, pBS2 i NPL-1. Mikrobiologiia. 1985 Sep-Oct;54(5):755–762. [PubMed] [Google Scholar]
  31. Tsuda M., Iino T. Naphthalene degrading genes on plasmid NAH7 are on a defective transposon. Mol Gen Genet. 1990 Aug;223(1):33–39. doi: 10.1007/BF00315794. [DOI] [PubMed] [Google Scholar]
  32. Wheatcroft R., Williams P. A. Rapid methods for the study of both stable and unstable plasmids in Pseudomonas. J Gen Microbiol. 1981 Jun;124(2):433–437. doi: 10.1099/00221287-124-2-433. [DOI] [PubMed] [Google Scholar]
  33. Wong C. L., Dunn N. W. Transmissible plasmid coding for the degradation of benzoate and m-toluate in Pseudomonas arvilla mt-2. Genet Res. 1974 Apr;23(2):227–232. doi: 10.1017/s0016672300014853. [DOI] [PubMed] [Google Scholar]
  34. Worsey M. J., Williams P. A. Metabolism of toluene and xylenes by Pseudomonas (putida (arvilla) mt-2: evidence for a new function of the TOL plasmid. J Bacteriol. 1975 Oct;124(1):7–13. doi: 10.1128/jb.124.1.7-13.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Yen K. M., Gunsalus I. C. Plasmid gene organization: naphthalene/salicylate oxidation. Proc Natl Acad Sci U S A. 1982 Feb;79(3):874–878. doi: 10.1073/pnas.79.3.874. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Yen K. M., Gunsalus I. C. Regulation of naphthalene catabolic genes of plasmid NAH7. J Bacteriol. 1985 Jun;162(3):1008–1013. doi: 10.1128/jb.162.3.1008-1013.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Yen K. M., Serdar C. M. Genetics of naphthalene catabolism in pseudomonads. Crit Rev Microbiol. 1988;15(3):247–268. doi: 10.3109/10408418809104459. [DOI] [PubMed] [Google Scholar]
  38. You I. S., Gunsalus I. C. Regulation of the nah and sal operons of plasmid NAH7: evidence for a new function in nahR. Biochem Biophys Res Commun. 1986 Dec 30;141(3):986–992. doi: 10.1016/s0006-291x(86)80141-3. [DOI] [PubMed] [Google Scholar]
  39. Zuniga M. C., Durham D. R., Welch R. A. Plasmid- and chromosome-mediated dissimilation of naphthalene and salicylate in Pseudomonas putida PMD-1. J Bacteriol. 1981 Sep;147(3):836–843. doi: 10.1128/jb.147.3.836-843.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]

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