Skip to main content
NCBI home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1994 Mar;60(3):1021–1024. doi: 10.1128/aem.60.3.1021-1024.1994

Evaluation of Procedures for Isolation of Nontuberculous Mycobacteria from Soil and Water

T Kamala 1, C N Paramasivan 1, Daniel Herbert 1, P Venkatesan 1, R Prabhakar 1,*
PMCID: PMC201427  PMID: 16349208

Abstract

Six methods of decontamination each for the isolation of mycobacteria from soil and water were compared. On the basis of the results obtained, three of the six methods for soil and two of the six methods for water were further evaluated. For both soil and water samples, the method using 3% sodium lauryl sulfate in combination with 1% NaOH yielded more positives than the other methods.

Full text

PDF
1021

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brooks R. W., Parker B. C., Gruft H., Falkinham J. O., 3rd Epidemiology of infection by nontuberculous mycobacteria. V. Numbers in eastern United States soils and correlation with soil characteristics. Am Rev Respir Dis. 1984 Oct;130(4):630–633. doi: 10.1164/arrd.1984.130.4.630. [DOI] [PubMed] [Google Scholar]
  2. Engel H. W., Berwald L. G., Havelaar A. H. The occurrence of Mycobacterium kansasii in tapwater. Tubercle. 1980 Mar;61(1):21–26. doi: 10.1016/0041-3879(80)90055-0. [DOI] [PubMed] [Google Scholar]
  3. Falkinham J. O., 3rd, Parker B. C., Gruft H. Epidemiology of infection by nontuberculous mycobacteria. I. Geographic distribution in the eastern United States. Am Rev Respir Dis. 1980 Jun;121(6):931–937. doi: 10.1164/arrd.1980.121.6.931. [DOI] [PubMed] [Google Scholar]
  4. George K. L., Falkinham J. O., 3rd Selective medium for the isolation and enumeration of Mycobacterium avium-intracellulare and M. scrofulaceum. Can J Microbiol. 1986 Jan;32(1):10–14. doi: 10.1139/m86-003. [DOI] [PubMed] [Google Scholar]
  5. Goslee S., Wolinsky E. Water as a source of potentially pathogenic mycobacteria. Am Rev Respir Dis. 1976 Mar;113(3):287–292. doi: 10.1164/arrd.1976.113.3.287. [DOI] [PubMed] [Google Scholar]
  6. Joseph S., Nair N. G., Gangadharam P. R. A sputum swab culture method for tubercle bacilli using cetrimide compared with two other swab culture methods and the concentration culture method. Tubercle. 1969 Sep;50(3):299–304. doi: 10.1016/0041-3879(69)90055-5. [DOI] [PubMed] [Google Scholar]
  7. Langerová M., Tacquet A. Comparaison de différentes méthodes d'homogénéisation et de purification des produits pathologiques en fonction de milieux de culture solides ou liquides. Bull World Health Organ. 1968;39(5):663–680. [PMC free article] [PubMed] [Google Scholar]
  8. Paramasivan C. N., Govindan D., Prabhakar R., Somasundaram P. R., Subbammal S., Tripathy S. P. Species level identification of non-tuberculous mycobacteria from South Indian BCG trial area during 1981. Tubercle. 1985 Mar;66(1):9–15. doi: 10.1016/0041-3879(85)90048-0. [DOI] [PubMed] [Google Scholar]
  9. Portaels F., De Muynck A., Sylla M. P. Selective isolation of mycobacteria from soil: a statistical analysis approach. J Gen Microbiol. 1988 Mar;134(3):849–855. doi: 10.1099/00221287-134-3-849. [DOI] [PubMed] [Google Scholar]
  10. Reznikov M., Leggo J. H. Examination of soil in the brisbane area for organisms of the Mycobacterium avium-intracellulare-scrofulaceum complex. Pathology. 1974 Jul;6(3):269–273. doi: 10.3109/00313027409068997. [DOI] [PubMed] [Google Scholar]
  11. Songer J. G. Methods for selective isolation of mycobacteria from the environment. Can J Microbiol. 1981 Jan;27(1):1–7. doi: 10.1139/m81-001. [DOI] [PubMed] [Google Scholar]
  12. Sula L. Comparative trials with different decontaminating agents for growing Mycobacterium tuberculosis from sputum specimens. Bull World Health Organ. 1968;39(5):647–655. [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES