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. 1994 Mar;60(3):1029–1032. doi: 10.1128/aem.60.3.1029-1032.1994

Characteristics of beta-lactamase-inhibiting proteins from Streptomyces exfoliatus SMF19.

M K Kim 1, K J Lee 1
PMCID: PMC201429  PMID: 8161170

Abstract

Streptomyces exfoliatus SMF19 produced two types of extracellular beta-lactamase-inhibiting proteins, 48 (beta LIP-I) and 33 (beta LIP-II) kDa. The inhibition mode of beta LIP-I on Bacto Penase and benzylpenicillin as substrates was uncompetitive (i.e., both Km and Vmax were changed), while that of beta LIP-II was noncompetitive (i.e., only Vmax was changed). The inhibition constants of beta LIP-I and beta LIP-II were 0.62 x 10(-4) and 2.74 x 10(-1) mumol, respectively. The N-terminal amino acid sequence of beta LIP-I was NH3-*-S-T-V-F-D-L-V-*-L-G, and that of beta LIP-II was NH3-D-F-*-V-F-D-L-E-A-T-D-E.

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Selected References

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  1. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  2. Brown A. G., Butterworth D., Cole M., Hanscomb G., Hood J. D., Reading C., Rolinson G. N. Naturally-occurring beta-lactamase inhibitors with antibacterial activity. J Antibiot (Tokyo) 1976 Jun;29(6):668–669. doi: 10.7164/antibiotics.29.668. [DOI] [PubMed] [Google Scholar]
  3. Butterworth D., Cole M., Hanscomb G., Rolinson G. N. Olivanic acids, a family of beta-lactam antibiotics with beta-lactamase inhibitory properties produced by Streptomyces species. I. Detection, properties and fermentation studies. J Antibiot (Tokyo) 1979 Apr;32(4):287–294. doi: 10.7164/antibiotics.32.287. [DOI] [PubMed] [Google Scholar]
  4. Doran J. L., Leskiw B. K., Aippersbach S., Jensen S. E. Isolation and characterization of a beta-lactamase-inhibitory protein from Streptomyces clavuligerus and cloning and analysis of the corresponding gene. J Bacteriol. 1990 Sep;172(9):4909–4918. doi: 10.1128/jb.172.9.4909-4918.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Duez C., Piron-Fraipont C., Joris B., Dusart J., Urdea M. S., Martial J. A., Frère J. M., Ghuysen J. M. Primary structure of the Streptomyces R61 extracellular DD-peptidase. 1. Cloning into Streptomyces lividans and nucleotide sequence of the gene. Eur J Biochem. 1987 Feb 2;162(3):509–518. doi: 10.1111/j.1432-1033.1987.tb10669.x. [DOI] [PubMed] [Google Scholar]
  6. Hata T., Omura S., Iwai Y., Ono H., Takeshima H. Studies on penicillinase inhibitors produced by microorganisms. J Antibiot (Tokyo) 1972 Aug;25(8):473–474. doi: 10.7164/antibiotics.25.473. [DOI] [PubMed] [Google Scholar]
  7. Joris B., Ghuysen J. M., Dive G., Renard A., Dideberg O., Charlier P., Frère J. M., Kelly J. A., Boyington J. C., Moews P. C. The active-site-serine penicillin-recognizing enzymes as members of the Streptomyces R61 DD-peptidase family. Biochem J. 1988 Mar 1;250(2):313–324. doi: 10.1042/bj2500313. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kahan J. S., Kahan F. M., Goegelman R., Currie S. A., Jackson M., Stapley E. O., Miller T. W., Miller A. K., Hendlin D., Mochales S. Thienamycin, a new beta-lactam antibiotic. I. Discovery, taxonomy, isolation and physical properties. J Antibiot (Tokyo) 1979 Jan;32(1):1–12. doi: 10.7164/antibiotics.32.1. [DOI] [PubMed] [Google Scholar]
  9. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  10. Matsudaira P. Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J Biol Chem. 1987 Jul 25;262(21):10035–10038. [PubMed] [Google Scholar]
  11. Ogawara H., Horikawa S., Shimada-Miyoshi S., Yasuzawa K. Production and property of beta-lactamases in Streptomyces: comparison of the strains isolated newly and thirty years ago. Antimicrob Agents Chemother. 1978 May;13(5):865–870. doi: 10.1128/aac.13.5.865. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Okamura K., Hirata S., Okumura Y., Fukagawa Y., Shimauchi Y., Kouno K., Ishikura T. PS-5, a new beta-lactam antibiotic from Streptomyces. J Antibiot (Tokyo) 1978 May;31(5):480–482. doi: 10.7164/antibiotics.31.480. [DOI] [PubMed] [Google Scholar]
  13. Reading C., Cole M. Clavulanic acid: a beta-lactamase-inhiting beta-lactam from Streptomyces clavuligerus. Antimicrob Agents Chemother. 1977 May;11(5):852–857. doi: 10.1128/aac.11.5.852. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sawai T., Takahashi I., Yamagishi S. Iodometric assay method for beta-lactamase with various beta-lactam antibiotics as substrates. Antimicrob Agents Chemother. 1978 Jun;13(6):910–913. doi: 10.1128/aac.13.6.910. [DOI] [PMC free article] [PubMed] [Google Scholar]

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