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. 1994 Apr;60(4):1191–1197. doi: 10.1128/aem.60.4.1191-1197.1994

Inhibition of Campylobacter jejuni colonization in chicks by defined competitive exclusion bacteria.

J L Schoeni 1, A C Wong 1
PMCID: PMC201458  PMID: 8017915

Abstract

Campylobacter enteritis in humans has been linked to consumption of chicken. Reducing Campylobacter jejuni colonization in chickens can potentially reduce Campylobacter infections in humans. In this study, the reduction of C. jejuni colonization in chicks by oral administration of defined competitive exclusion (CE) cultures, 2.5% dietary carbohydrates, or CE cultures and carbohydrates was examined. Prevention, elimination, or direct challenge of Campylobacter infection was simulated by administering treatments before, after, or at the same time as that of the Campylobacter inoculation. Additionally, the effect of maintaining high levels of protective bacteria was evaluated by administering CE cultures on days 1 and 4 (booster treatment). All treatments reduced C. jejuni colonization. Protection by aerobically grown CE cultures was not statistically different from that by anaerobically grown CE cultures. A combination of Citrobacter diversus 22, Klebsiella pneumoniae 23, and Escherichia coli 25 (CE 3) was the most effective CE treatment. Maintaining high numbers of CE isolates by administering CE boosters did not increase protection. The greatest reduction of Campylobacter colonization was observed in schemes to prevent or eliminate C. jejuni infection. C. jejuni was not detected in the ceca of birds receiving the prevention treatment, CE 3 with mannose, representing a 62% reduction in the colonization rate. The protection factor (PF), a value combining the colonization rate and the level of infection, for CE 3 with mannose was high (> 13.2). Fructo-oligosaccharides alone strongly prevented Campylobacter colonization. Only 8% of the chicks in this group were colonized, with a PF of > 14.3.(ABSTRACT TRUNCATED AT 250 WORDS)

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Selected References

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  1. Aho M., Nuotio L., Nurmi E., Kiiskinen T. Competitive exclusion of campylobacters from poultry with K-bacteria and Broilact. Int J Food Microbiol. 1992 Mar-Apr;15(3-4):265–275. doi: 10.1016/0168-1605(92)90057-a. [DOI] [PubMed] [Google Scholar]
  2. Bailey J. S., Blankenship L. C., Cox N. A. Effect of fructooligosaccharide on Salmonella colonization of the chicken intestine. Poult Sci. 1991 Dec;70(12):2433–2438. doi: 10.3382/ps.0702433. [DOI] [PubMed] [Google Scholar]
  3. Blaser M. J., Taylor D. N., Feldman R. A. Epidemiology of Campylobacter jejuni infections. Epidemiol Rev. 1983;5:157–176. doi: 10.1093/oxfordjournals.epirev.a036256. [DOI] [PubMed] [Google Scholar]
  4. Brouwer R., Mertens M. J., Siem T. H., Katchaki J. An explosive outbreak of Campylobacter enteritis in soldiers. Antonie Van Leeuwenhoek. 1979;45(3):517–519. doi: 10.1007/BF00443293. [DOI] [PubMed] [Google Scholar]
  5. Butzler J. P., Skirrow M. B. Campylobacter enteritis. Clin Gastroenterol. 1979 Sep;8(3):737–765. [PubMed] [Google Scholar]
  6. Deming M. S., Tauxe R. V., Blake P. A., Dixon S. E., Fowler B. S., Jones T. S., Lockamy E. A., Patton C. M., Sikes R. O. Campylobacter enteritis at a university: transmission from eating chicken and from cats. Am J Epidemiol. 1987 Sep;126(3):526–534. doi: 10.1093/oxfordjournals.aje.a114685. [DOI] [PubMed] [Google Scholar]
  7. Hopkins R. S., Olmsted R., Istre G. R. Endemic Campylobacter jejuni infection in Colorado: identified risk factors. Am J Public Health. 1984 Mar;74(3):249–250. doi: 10.2105/ajph.74.3.249. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hopkins R. S., Scott A. S. Handling raw chicken as a source for sporadic Campylobacter jejuni infections. J Infect Dis. 1983 Oct;148(4):770–770. doi: 10.1093/infdis/148.4.770. [DOI] [PubMed] [Google Scholar]
  9. Istre G. R., Blaser M. J., Shillam P., Hopkins R. S. Campylobacter enteritis associated with undercooked barbecued chicken. Am J Public Health. 1984 Nov;74(11):1265–1267. doi: 10.2105/ajph.74.11.1265. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McSweegan E., Walker R. I. Identification and characterization of two Campylobacter jejuni adhesins for cellular and mucous substrates. Infect Immun. 1986 Jul;53(1):141–148. doi: 10.1128/iai.53.1.141-148.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mitsuoka T., Hidaka H., Eida T. Effect of fructo-oligosaccharides on intestinal microflora. Nahrung. 1987;31(5-6):427–436. doi: 10.1002/food.19870310528. [DOI] [PubMed] [Google Scholar]
  12. Norkrans G., Svedhem A. Epidemiological aspects of Campylobacter jejuni enteritis. J Hyg (Lond) 1982 Aug;89(1):163–170. doi: 10.1017/s0022172400070662. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Nurmi E., Rantala M. New aspects of Salmonella infection in broiler production. Nature. 1973 Jan 19;241(5386):210–211. doi: 10.1038/241210a0. [DOI] [PubMed] [Google Scholar]
  14. Rettig P. J. Campylobacter infections in human beings. J Pediatr. 1979 Jun;94(6):855–864. doi: 10.1016/s0022-3476(79)80202-4. [DOI] [PubMed] [Google Scholar]
  15. Rosenfield J. A., Arnold G. J., Davey G. R., Archer R. S., Woods W. H. Serotyping of Campylobacter jejuni from an outbreak of enteritis implicating chicken. J Infect. 1985 Sep;11(2):159–165. doi: 10.1016/s0163-4453(85)92123-1. [DOI] [PubMed] [Google Scholar]
  16. Schoeni J. L., Doyle M. P. Reduction of Campylobacter jejuni colonization of chicks by cecum-colonizing bacteria producing anti-C. jejuni metabolites. Appl Environ Microbiol. 1992 Feb;58(2):664–670. doi: 10.1128/aem.58.2.664-670.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Shanker S., Lee A., Sorrell T. C. Experimental colonization of broiler chicks with Campylobacter jejuni. Epidemiol Infect. 1988 Feb;100(1):27–34. doi: 10.1017/s0950268800065523. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Shanker S., Lee A., Sorrell T. C. Horizontal transmission of Campylobacter jejuni amongst broiler chicks: experimental studies. Epidemiol Infect. 1990 Feb;104(1):101–110. doi: 10.1017/s0950268800054571. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Soerjadi-Liem A. S., Snoeyenbos G. H., Weinack O. M. Comparative studies on competitive exclusion of three isolates of Campylobacter fetus subsp. jejuni in chickens by native gut microflora. Avian Dis. 1984 Jan-Mar;28(1):139–146. [PubMed] [Google Scholar]
  20. Soerjadi A. S., Snoeyenbos G. H., Weinack O. M. Intestinal colonization and competitive exclusion of Campylobacter fetus subsp. jejuni in young chicks. Avian Dis. 1982 Jul-Sep;26(3):520–524. [PubMed] [Google Scholar]
  21. Stavric S. Defined cultures and prospects. Int J Food Microbiol. 1992 Mar-Apr;15(3-4):245–263. doi: 10.1016/0168-1605(92)90056-9. [DOI] [PubMed] [Google Scholar]
  22. Stern N. J., Bailey J. S., Blankenship L. C., Cox N. A., McHan F. Colonization characteristics of Campylobacter jejuni in chick ceca. Avian Dis. 1988 Apr-Jun;32(2):330–334. [PubMed] [Google Scholar]
  23. Tauxe R. V., Hargrett-Bean N., Patton C. M., Wachsmuth I. K. Campylobacter isolates in the United States, 1982-1986. MMWR CDC Surveill Summ. 1988 Jun;37(2):1–13. [PubMed] [Google Scholar]

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