Abstract
The small subunit (SSU) and the internal transcribed spacer (ITS) of nuclear ribosomal DNA genes from 27 specimens of the fungal genera Gremmeniella and Ascocalyx were amplified by PCR. Length polymorphisms were observed in the SSU and allowed the differentiation of four groups among the isolates tested: (i) Ascocalyx abietis; (ii) Gremmeniella isolates from Picea spp.; (iii) Gremmeniella isolates from Abies balsamea; and (iv) Gremmeniella isolates from Abies sacchalinensis, Larix spp., and Pinus spp. The amplified ITS was the same length for all Gremmeniella specimens and was 60 bp longer in A. abietis. Phylogenetic analysis of length polymorphisms and of 24 restriction sites in the SSU and ITS showed that Gremmeniella isolates were more related to each other than to the Ascocalyx isolate. Furthermore, seven groups were evident within the genus Gremmeniella. Our results confirm that Gremmeniella and Ascocalyx should be kept as different taxa and suggest that the taxonomy of the former could be revised to consider isolates from Abies balsamea and from Picea spp. to be two different varieties while incorporating Gremmeniella laricina into G. abietina, as a new variety.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- De Wachter R., Neefs J. M., Goris A., Van de Peer Y. The gene coding for small ribosomal subunit RNA in the basidiomycete Ustilago maydis contains a group I intron. Nucleic Acids Res. 1992 Mar 25;20(6):1251–1257. doi: 10.1093/nar/20.6.1251. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DePriest P. T., Been M. D. Numerous group I introns with variable distributions in the ribosomal DNA of a lichen fungus. J Mol Biol. 1992 Nov 20;228(2):315–321. doi: 10.1016/0022-2836(92)90819-6. [DOI] [PubMed] [Google Scholar]
- Hamelin R. C., Ouellette G. B., Bernier L. Identification of Gremmeniella abietina Races with Random Amplified Polymorphic DNA Markers. Appl Environ Microbiol. 1993 Jun;59(6):1752–1755. doi: 10.1128/aem.59.6.1752-1755.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Simon L., Lalonde M., Bruns T. D. Specific amplification of 18S fungal ribosomal genes from vesicular-arbuscular endomycorrhizal fungi colonizing roots. Appl Environ Microbiol. 1992 Jan;58(1):291–295. doi: 10.1128/aem.58.1.291-295.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sogin M. L., Edman J. C. A self-splicing intron in the small subunit rRNA gene of Pneumocystis carinii. Nucleic Acids Res. 1989 Jul 11;17(13):5349–5359. doi: 10.1093/nar/17.13.5349. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zolan M. E., Pukkila P. J. Inheritance of DNA methylation in Coprinus cinereus. Mol Cell Biol. 1986 Jan;6(1):195–200. doi: 10.1128/mcb.6.1.195. [DOI] [PMC free article] [PubMed] [Google Scholar]