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. 1994 Apr;60(4):1383–1386. doi: 10.1128/aem.60.4.1383-1386.1994

A Simple Method That Uses Differential Staining and Light Microscopy To Assess the Selectivity of Wood Delignification by White Rot Fungi

Ewald Srebotnik 1,*, Kurt Messner 1
PMCID: PMC201488  PMID: 16349245

Abstract

Cryostat microtome sections of birch wood degraded by white rot fungi were examined by light microscopy after treatment with two stains: astra-blue, which stains cellulose blue only in the absence of lignin, and safranin, which stains lignin regardless of whether cellulose is present. The method provided a simple and reliable screening procedure that distinguishes between fungi that cause decay by selectively removing lignin and those that degrade both cellulose and lignin simultaneously. Moreover, morphological characteristics specific to selective delignification were revealed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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