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. 1994 May;60(5):1614–1621. doi: 10.1128/aem.60.5.1614-1621.1994

Cultural and phylogenetic analysis of mixed microbial populations found in natural and commercial bioleaching environments.

B M Goebel 1, E Stackebrandt 1
PMCID: PMC201525  PMID: 7517131

Abstract

A range of autotrophic and heterotrophic enrichment cultures were established to determine the cultural bacterial diversity present in samples obtained from the acidic runoff of a chalcocite overburden heap and from laboratory-scale (1- to 4-liter) batch and continuous bioreactors which were being used for the commercial assessment of the bioleachability of zinc sulfide ore concentrates. Strains identified as Thiobacillus ferrooxidans, Thiobacillus thiooxidans, "Leptospirillum ferrooxidans," and Acidiphilium cryptum were isolated from both the natural site and the batch bioreactor, but only "L. ferrooxidans," a moderately thermophilic strain of T. thiooxidans, and a moderately thermophilic iron-oxidizing bacterium could be recovered from the continuous bioreactor running under steady-state conditions. Sequence analysis of the 16S rRNA genes of 33 representative strains revealed that all of the strains were closely related to strains which have been sequenced previously and also confirmed the phylogenetic diversity of bacteria present in bioleaching environments.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amann R. I., Stromley J., Devereux R., Key R., Stahl D. A. Molecular and microscopic identification of sulfate-reducing bacteria in multispecies biofilms. Appl Environ Microbiol. 1992 Feb;58(2):614–623. doi: 10.1128/aem.58.2.614-623.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Blake R. C., 2nd, Shute E. A., Greenwood M. M., Spencer G. H., Ingledew W. J. Enzymes of aerobic respiration on iron. FEMS Microbiol Rev. 1993 Jul;11(1-3):9–18. doi: 10.1111/j.1574-6976.1993.tb00261.x. [DOI] [PubMed] [Google Scholar]
  3. Brierley C. L. Bacterial leaching. CRC Crit Rev Microbiol. 1978;6(3):207–26I. doi: 10.3109/10408417809090623. [DOI] [PubMed] [Google Scholar]
  4. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci U S A. 1978 Oct;75(10):4801–4805. doi: 10.1073/pnas.75.10.4801. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. COLMER A. R., TEMPLE K. L., HINKLE M. E. An iron-oxidizing bacterium from the acid drainage of some bituminous coal mines. J Bacteriol. 1950 Mar;59(3):317–328. doi: 10.1128/jb.59.3.317-328.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Guay R., Silver M. Thiobacillus acidophilus sp. nov.; isolation and some physiological characteristics. Can J Microbiol. 1975 Mar;21(3):281–288. doi: 10.1139/m75-040. [DOI] [PubMed] [Google Scholar]
  7. Harrison A. P., Jr, Jarvis B. W., Johnson J. L. Heterotrophic bacteria from cultures of autotrophic Thiobacillus ferrooxidans: relationships as studied by means of deoxyribonucleic acid homology. J Bacteriol. 1980 Jul;143(1):448–454. doi: 10.1128/jb.143.1.448-454.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Harrison A. P., Jr Microbial succession and mineral leaching in an artificial coal spoil. Appl Environ Microbiol. 1978 Dec;36(6):861–869. doi: 10.1128/aem.36.6.861-869.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Harrison A. P., Jr The acidophilic thiobacilli and other acidophilic bacteria that share their habitat. Annu Rev Microbiol. 1984;38:265–292. doi: 10.1146/annurev.mi.38.100184.001405. [DOI] [PubMed] [Google Scholar]
  10. Ingledew W. J. Thiobacillus ferrooxidans. The bioenergetics of an acidophilic chemolithotroph. Biochim Biophys Acta. 1982 Nov 30;683(2):89–117. doi: 10.1016/0304-4173(82)90007-6. [DOI] [PubMed] [Google Scholar]
  11. Kane M. D., Poulsen L. K., Stahl D. A. Monitoring the enrichment and isolation of sulfate-reducing bacteria by using oligonucleotide hybridization probes designed from environmentally derived 16S rRNA sequences. Appl Environ Microbiol. 1993 Mar;59(3):682–686. doi: 10.1128/aem.59.3.682-686.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lane D. J., Harrison A. P., Jr, Stahl D., Pace B., Giovannoni S. J., Olsen G. J., Pace N. R. Evolutionary relationships among sulfur- and iron-oxidizing eubacteria. J Bacteriol. 1992 Jan;174(1):269–278. doi: 10.1128/jb.174.1.269-278.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lane D. J., Stahl D. A., Olsen G. J., Heller D. J., Pace N. R. Phylogenetic analysis of the genera Thiobacillus and Thiomicrospira by 5S rRNA sequences. J Bacteriol. 1985 Jul;163(1):75–81. doi: 10.1128/jb.163.1.75-81.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ochman H., Wilson A. C. Evolution in bacteria: evidence for a universal substitution rate in cellular genomes. J Mol Evol. 1987;26(1-2):74–86. doi: 10.1007/BF02111283. [DOI] [PubMed] [Google Scholar]
  15. Olsen G. J., Lane D. J., Giovannoni S. J., Pace N. R., Stahl D. A. Microbial ecology and evolution: a ribosomal RNA approach. Annu Rev Microbiol. 1986;40:337–365. doi: 10.1146/annurev.mi.40.100186.002005. [DOI] [PubMed] [Google Scholar]
  16. Olsen G. J., Overbeek R., Larsen N., Marsh T. L., McCaughey M. J., Maciukenas M. A., Kuan W. M., Macke T. J., Xing Y., Woese C. R. The Ribosomal Database Project. Nucleic Acids Res. 1992 May 11;20 (Suppl):2199–2200. doi: 10.1093/nar/20.suppl.2199. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Poulsen L. K., Ballard G., Stahl D. A. Use of rRNA fluorescence in situ hybridization for measuring the activity of single cells in young and established biofilms. Appl Environ Microbiol. 1993 May;59(5):1354–1360. doi: 10.1128/aem.59.5.1354-1360.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Skerman V. B. A new type of micromanipulator and microforge. J Gen Microbiol. 1968 Dec;54(2):287–297. doi: 10.1099/00221287-54-2-287. [DOI] [PubMed] [Google Scholar]
  19. Southam G., Beveridge T. J. Enumeration of Thiobacilli within pH-Neutral and Acidic Mine Tailings and Their Role in the Development of Secondary Mineral Soil. Appl Environ Microbiol. 1992 Jun;58(6):1904–1912. doi: 10.1128/aem.58.6.1904-1912.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Southam G., Beveridge T. J. Examination of Lipopolysaccharide (O-Antigen) Populations of Thiobacillus ferrooxidans from Two Mine Tailings. Appl Environ Microbiol. 1993 May;59(5):1283–1288. doi: 10.1128/aem.59.5.1283-1288.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Woese C. R. Bacterial evolution. Microbiol Rev. 1987 Jun;51(2):221–271. doi: 10.1128/mr.51.2.221-271.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]

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