Abstract
In a fermentor, a Mycobacterium sp. was grown on pyrene at defined oxygen concentrations in a range from 11.4 to 227 microM. The maximal growth rate (mumax = 0.057 h-1) and the dissolved oxygen half-saturation constant (KDO = 5.9 microM) were calculated. At 3.4 microM, the growth rate (mu = 0.011 h-1) was only half of what was expected from the kinetic data. Apparently, this was due to limitation of an oxygenase of pyrene degradation.
Full text
PDF![1687](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96bb/201540/b1dc1c55f956/aem00022-0305.png)
![1688](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96bb/201540/23c60f843046/aem00022-0306.png)
![1689](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96bb/201540/21ba97617e2d/aem00022-0307.png)
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Boldrin B., Tiehm A., Fritzsche C. Degradation of phenanthrene, fluorene, fluoranthene, and pyrene by a Mycobacterium sp. Appl Environ Microbiol. 1993 Jun;59(6):1927–1930. doi: 10.1128/aem.59.6.1927-1930.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heitkamp M. A., Freeman J. P., Miller D. W., Cerniglia C. E. Pyrene degradation by a Mycobacterium sp.: identification of ring oxidation and ring fission products. Appl Environ Microbiol. 1988 Oct;54(10):2556–2565. doi: 10.1128/aem.54.10.2556-2565.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Johnson M. J. Aerobic microbial growth at low oxygen concentrations. J Bacteriol. 1967 Jul;94(1):101–108. doi: 10.1128/jb.94.1.101-108.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LONGMUIR I. S. Respiration rate of bacteria as a function of oxygen concentration. Biochem J. 1954 May;57(1):81–87. doi: 10.1042/bj0570081. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Michaelsen M., Hulsch R., Höpner T., Berthe-Corti L. Hexadecane mineralization in oxygen-controlled sediment-seawater cultivations with autochthonous microorganisms. Appl Environ Microbiol. 1992 Sep;58(9):3072–3077. doi: 10.1128/aem.58.9.3072-3077.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mihelcic J. R., Luthy R. G. Microbial degradation of acenaphthene and naphthalene under denitrification conditions in soil-water systems. Appl Environ Microbiol. 1988 May;54(5):1188–1198. doi: 10.1128/aem.54.5.1188-1198.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shaler T. A., Klecka G. M. Effects of dissolved oxygen concentration on biodegradation of 2,4-dichlorophenoxyacetic acid. Appl Environ Microbiol. 1986 May;51(5):950–955. doi: 10.1128/aem.51.5.950-955.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]