Abstract
During an evaluation of the Accuprobe kit for the detection of Listeria monocytogenes, some of the enrichment media used were found to interfere with the test. Microscopic examination during the lysis step of the test revealed that media containing high salt greatly reduced or prevented cell lysis. This prevented the probe from binding to the cellular RNA, resulting in false-negative results.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Metcalf R. H., Deibel R. H. Differential lytic response of enterococci associated with addition order of lysozyme and anions. J Bacteriol. 1969 Sep;99(3):674–680. doi: 10.1128/jb.99.3.674-680.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ninet B., Bannerman E., Bille J. Assessment of the Accuprobe Listeria monocytogenes culture identification reagent kit for rapid colony confirmation and its application in various enrichment broths. Appl Environ Microbiol. 1992 Dec;58(12):4055–4059. doi: 10.1128/aem.58.12.4055-4059.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]